Data for New cold-adapted bacteria for efficient hydrolysis of feather waste at low temperature paper ...

• Main Authors: Smirnova, Margarita, Losada, Cristian Bolaño, Akulava, Volha, Zimmermann, Boris, Kohler, Achim, Miamin, Uladzislau, Oostindjer, Marije, Shapaval, Volha
• Format: Dataset
• Language: unknown
• Published: Zenodo 2023
• Subjects: Antarctic bacteria, feather degradation, enzymes, keratinase, spectroscopy; Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.5281/zenodo.7769804; https://zenodo.org/record/7769804

A novel cold-adapted bacteria Arthrobacter oryzae BIM B-1663 isolated from Antarctic green snow showed keratinase activity and efficient poultry feather degradation. A. oryzae strain degraded more than 80% of chicken feathers within 7 days of cultivation at 25°C. The optimal keratinase activity for A. oryzae BIM B-1663 was observed at 50°C, both for α-keratin (44.86 U/mL) and for β-keratin (94 mU/mL). The obtained results from sulfite and thiol groups tests and Fourier transform infrared spectroscopy (FTIR) showed that A. oryzae strain has a different keratin degradation mechanism than the reference strain Bacillus licheniformis CCM 2145 T . FTIR fingerprinting can be used for monitoring of feather hydrolysis as it showed distinct chemical differences in feather meal hydrolysates, retentate and permeate from A. oryzae and B. licheniformis strains. ...