Permafrost-thaw lake development in Central Yakutia: sedimentary ancient DNA and element analyses from a Holocene sediment record ...

• Main Authors: Baisheva, Izabella, Levina, Sardana, Biskaborn, Boris K., Vyse, Stuart A., Heim, Birgit, Pestryakova, Luidmila, Glückler, Ramesh, Herzschuh, Ulrike, Stoof-Leichsenring, Kathleen R.
• Format: Software
• Language: unknown
• Published: Zenodo 2022
• Subjects: sedaDNA; metabarcoding; lake sediment core; Central Yakutia; Siberia; Permafrost; alaas; thaw-lake; Diatoms; Plants; macrophytes; Earth-Surface Processes; Aquatic Science; Alaas; permafrost; Thermokarst; Yakutia
• Online Access: https://dx.doi.org/10.5281/zenodo.6937268; https://zenodo.org/record/6937268

In Central Yakutia (Siberia) livelihoods of local communities depend on alaas (thermokarst depression) landscapes and the lakes within. Development and dynamics of these alaas lakes are closely connected to climate change, permafrost thawing, catchment conditions, and land use. To reconstruct lake development throughout the Holocene we analyze sedimentary ancient DNA (sedaDNA) and biogeochemistry from a sediment core from Lake Satagay, spanning the last c. 10,800 calibrated years before present (cal yrs BP). SedaDNA of diatoms and macrophytes and microfossil diatom analysis reveal lake formation earlier than 10,700 cal yrs BP. The sedaDNA approach detected 42 amplicon sequence variants (ASVs) of diatom taxa, one ASV of Eustigmatophyceae (Nannochloropsis), and 12 ASVs of macrophytes. We relate diatom and macrophyte community changes to climate-driven shifts in water level and mineral and organic input, which result in variable water conductivity, in-lake productivity, and sediment deposition. We detect a ... : The datasets* are prepared for the manuscripts: Baisheva et al. (2022): "Permafrost-thaw lake development in Central Yakutia – Sedimentary ancient DNA and element analyses from a Holocene sediment record" (submitted) and Glückler et al. (2022): "Holocene wildfire and vegetation dynamics in Central Yakutia, Siberia, reconstructed from lake-sediment proxies" (preprint). Also included is the processing of the raw sequencing data using bioinformatics tools. *The datasets were uploaded into two separate directories containing data and scripts. Each directory contains two main folders APMG_32 (Diatoms) and APMG_33 (Plants). Files of APMG_32 and APMG_33 after downloading have to be merged in the same folder, so the structure of datasets looks like as it is given below: 1) APMG_32 contains several folders and files of different format: 00. APMG-32_Metadata - Metadata information including lake geographic coordinates, sample depths and ages, laboratory codes and used primer tag combinations of forward and reverse ...