Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet
Conservation strategies centred around species habitat protection rely on species' dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie ( Tetrao urogallus ), which is an indicator species for forest biodiversity conserva...
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ftdatacite:10.5281/zenodo.5036296 2023-05-15T16:13:45+02:00 Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet Chua, Physilia Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger 2021 https://dx.doi.org/10.5281/zenodo.5036296 https://zenodo.org/record/5036296 unknown Zenodo https://zenodo.org/communities/dryad https://dx.doi.org/10.1101/2021.03.08.434346 https://dx.doi.org/10.5061/dryad.dfn2z3524 https://dx.doi.org/10.5281/zenodo.5036295 https://zenodo.org/communities/dryad Open Access MIT License https://opensource.org/licenses/MIT mit info:eu-repo/semantics/openAccess MIT Software SoftwareSourceCode article 2021 ftdatacite https://doi.org/10.5281/zenodo.5036296 https://doi.org/10.1101/2021.03.08.434346 https://doi.org/10.5061/dryad.dfn2z3524 https://doi.org/10.5281/zenodo.5036295 2021-11-05T12:55:41Z Conservation strategies centred around species habitat protection rely on species' dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie ( Tetrao urogallus ), which is an indicator species for forest biodiversity conservation. Non-molecular means used to study their diet are time-consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here we use non-invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faeces to present the first large-scale molecular dietary analysis of capercaillies. Faecal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n=172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus , respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F 5,86 = 11.01, r 2 = 0.17, p = 0.001) and seasons (adonis pseudo F 2,03 = 0.64, r 2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F 1,47 = 2.77, r 2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared to existing knowledge from non-molecular means. The non-invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies' diet, improving our understanding of adequate habitat required for their conservation. : Funding provided by: Horizon 2020 Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100007601 Award Number: 765000 Article in Journal/Newspaper Finnmark Finnmark Troms DataCite Metadata Store (German National Library of Science and Technology) Jura ENVELOPE(13.501,13.501,68.062,68.062) Norway |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
description |
Conservation strategies centred around species habitat protection rely on species' dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie ( Tetrao urogallus ), which is an indicator species for forest biodiversity conservation. Non-molecular means used to study their diet are time-consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here we use non-invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faeces to present the first large-scale molecular dietary analysis of capercaillies. Faecal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n=172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus , respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F 5,86 = 11.01, r 2 = 0.17, p = 0.001) and seasons (adonis pseudo F 2,03 = 0.64, r 2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F 1,47 = 2.77, r 2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared to existing knowledge from non-molecular means. The non-invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies' diet, improving our understanding of adequate habitat required for their conservation. : Funding provided by: Horizon 2020 Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100007601 Award Number: 765000 |
format |
Article in Journal/Newspaper |
author |
Chua, Physilia Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger |
spellingShingle |
Chua, Physilia Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
author_facet |
Chua, Physilia Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger |
author_sort |
Chua, Physilia |
title |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_short |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_fullStr |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full_unstemmed |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_sort |
molecular dietary analyses of western capercaillies (tetrao urogallus) reveal a diverse diet |
publisher |
Zenodo |
publishDate |
2021 |
url |
https://dx.doi.org/10.5281/zenodo.5036296 https://zenodo.org/record/5036296 |
long_lat |
ENVELOPE(13.501,13.501,68.062,68.062) |
geographic |
Jura Norway |
geographic_facet |
Jura Norway |
genre |
Finnmark Finnmark Troms |
genre_facet |
Finnmark Finnmark Troms |
op_relation |
https://zenodo.org/communities/dryad https://dx.doi.org/10.1101/2021.03.08.434346 https://dx.doi.org/10.5061/dryad.dfn2z3524 https://dx.doi.org/10.5281/zenodo.5036295 https://zenodo.org/communities/dryad |
op_rights |
Open Access MIT License https://opensource.org/licenses/MIT mit info:eu-repo/semantics/openAccess |
op_rightsnorm |
MIT |
op_doi |
https://doi.org/10.5281/zenodo.5036296 https://doi.org/10.1101/2021.03.08.434346 https://doi.org/10.5061/dryad.dfn2z3524 https://doi.org/10.5281/zenodo.5036295 |
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1765999603529809920 |