Perlodes floridus Kovacs & Vincon 2012, sp. n.

Perlodes floridus Kovács & Vinçon sp. n. (Figs. 1-3 ab, 4a, 5a, 6-7, 8-11, 16e) Material examined. Holotype: MONTENEGRO, Podgorica municipality, Gornji Mileš, Rogamsko brdo, Cijevna River, N42°23’59.7”, E19°18’42.6”, 65 m a.s.l., 25.05.2009, 1♀ +O (Figs. 8-9, 11, 16e), KT-MG-UL, MM: 2...

Full description

Bibliographic Details
Main Authors: Kovács, Tibor, Vinçon, Gilles, Murányi, Dávid, Sivec, Ignac
Format: Text
Language:unknown
Published: Zenodo 2012
Subjects:
Online Access:https://dx.doi.org/10.5281/zenodo.4758140
https://zenodo.org/record/4758140
id ftdatacite:10.5281/zenodo.4758140
record_format openpolar
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language unknown
topic Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Plecoptera
Perlodidae
Perlodes
Perlodes floridus
spellingShingle Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Plecoptera
Perlodidae
Perlodes
Perlodes floridus
Kovács, Tibor
Vinçon, Gilles
Murányi, Dávid
Sivec, Ignac
Perlodes floridus Kovacs & Vincon 2012, sp. n.
topic_facet Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Plecoptera
Perlodidae
Perlodes
Perlodes floridus
description Perlodes floridus Kovács & Vinçon sp. n. (Figs. 1-3 ab, 4a, 5a, 6-7, 8-11, 16e) Material examined. Holotype: MONTENEGRO, Podgorica municipality, Gornji Mileš, Rogamsko brdo, Cijevna River, N42°23’59.7”, E19°18’42.6”, 65 m a.s.l., 25.05.2009, 1♀ +O (Figs. 8-9, 11, 16e), KT-MG-UL, MM: 2009-49. Paratypes: same locality and date: 2 E, 1♂ (Figs. 3 ab), KT-MG-UL, MM: 2009-49; Dinoša, Cijevna River, N42°24’24.6”, E19°20’56.1”, 85 m, 29.05.2009, 1♂, 1♀ +O, (Figs. 1-2), KT-MG-UL, MM: 2009-66; 13.04.2010, 6L (Figs. 6-7), KT-MG-UL, MM: 2010-31. Zlatica, Morača River, N42°28’40.2”, E19°18’19.8”, 50 m, 2010.04.17., 3L, KT- MG-UL, MM: 2010-40. ALBANIA, Librazhd district, Qukës Shkumbin, Shkumbin River, N41°05’45.7”, E20°26’34.0”, 380 m, (2006/4), 09.04.2006, 1 E, EZ-FZ-HA-MD, HNHM: PLP 2184; Pukë district, Mertur, Mertur Stream at the influence to Koman Lake, N42°13’36.8”, E19°54’18.0”, 180 m, (2006/56), 15.04.2006, 1♀ +O, EZ-FZ-HA-MD, HNHM: PLP 2246; Sarandë district, Çikë Mts, Borsh, Borsh River N of the village, N40°03’57.1”, E19°50’53.5”, 35 m, (2008/18), 12.03.2008, 1E, 1♂, CSz-MD, HNHM: PLP 2525. GREECE, West Macedonia, Grevena regional unit, Aetia, Venetikos River, N40°04’27.9”, E21°12’06.0”, 973 m, (2006/114), 14.05.2006, 1♀ +O, DL-KJ-MD, HNHM: PLP 2135; Eleftherohori, Venetikos River, 24.04.1989, 1♀ +O, HB-SI, PMSL: 03220; N40°03’06.6”, E21°28’50.2”, 475 m, (2006/111), 13.05.2006, 1E, 1♀ +O, DL-KJ-MD, HNHM: PLP 2132; Kipourio, Venetikos River, 24.04.1989, 9E, 1♂, HB-SI, PMSL; Zakas, Venetikos River, N40°02’19.3”, E21°17’19.2”, 699 m, (2006/112), 14.05.2006, 1L, 1♂ (Figs. 4a, 5a), DL-KJ-MD, HNHM: PLP 2144; Thessaly, Trikala regional unit, Ambelia, Kalambaka, 20.04.1989, 9E, 6♂, 6♀, HB-SI (8E, 4♂, 4♀, O, PMSL: 03148; 1E, 2♂, 2♀, O (Fig. 10), CGV); Kastania, Kalambaka, 20.04.1989, 7E, 1♂, HB-SI, PMSL: 03141; Kato Palagokaria, 19.04.1989, 2♀ +O, HB-SI, PMSL: 03119; Longiai, 19.04.1989, 3♀ +O, (in very poor condition), HB-SI, PMSL: 03126; Karditsa regional unit, Sarantaporo, small river S of the village, N39°09’13.9”, E21°49’50.7”, 700 m, (2011/39), 08.05.2011, 1♂, KJ-MD-SzT-UZs, HNHM: PLP 3680; Central Greece, Phthiotis regional unit, Agios Georgios, Sperchios River W of the village, N38°57’00.5”, E21°56’42.7”, 365 m, (2011/35), 08.05.2011, 1♂, 1♀ +O, KJ-MD-SzT-UZs, HNHM: PLP 3669; Timfristos, Sperchios River, (207), 29.04.1989, 1♀ +O, HB-SI, PMSL; Phocis regional unit, Vardousia Mts, Stromi, 26.04.1990, 1E, 1♀ +O, HB-SI, PMSL: 03212; Giona Mts, Lefkaditi, NW of Amfissa, 15.04.1987, 4♂, 2♀, O, Drosopoulos, PMSL: 03300. Diagnosis . Head of the imago lacks yellow pattern by the M-line. Male macropterous, paraproct sclerite with long and straight apical part, apex angled. Female subgenital plate narrow. Larval hind femur long and narrow, last sternites with distinct setation. Egg with flower-like anchor, shape egg-like with cutoff base, FCIs distinct only on opercular part. Description (Figs. 1-2). Dark brown with distinct yellow markings. A small sized Perlodes . Body length: males 12.5-18 mm, females 14.5-20.5 mm; forewing length: males 11.5-14.5, females 15-19.5 mm. Dorsal part of head brown with two large pale patches (Fig. 1). One is a crown or tulip-shaped pattern on the occiput that laterally reaches the midline of the dark compound eyes, then deeply incised while central part pointed up to the postfrontal suture. Anterior pale patch is oval, delimited by the three, yellow ocelli. Occipital rugosities, tentorial callosities and M-line indistinct. Ventral part of head yellow, antennae and palpi brown. Pronotum brown with a median longitudinal yellow stripe (Fig. 1), prothorax ventrally yellow besides dark brown rhomboid or arrow-shaped dark patch anterior to furcasternum. Dorsal part of meso- and metathorax dark brown, ventrally mostly dark brownish. Legs all brown, wings normal sized, veins dark brown. Abdominal segments 1-4 divided by pleural membrane (as well as in larvae). Abdomen dorsally brown; males bear transverse pale patches on tergites IX–X, paired medial spots on tergite VIII, these may be present also on tergites VI–VII. Abdominal sternites brown, gradually darkened towards apex in males (transverse pale patches may be present medially); in females generally yellow while sternite I brown, sternites II-VII with a large anteromedial and variable lateral brown patches. Cerci brown in both sexes. Male genitalia (Figs. 3a, b, 4a, 5a). Tergite X undivided, not raised. Paraproctal sclerite in ventral view with long and straight apical part that gradually narrows towards its apex; sides parallel and keeled beneath the apex that is strongly angled (120°) and sharply pointed (Figs. 3a, 4a). Apical part of paraproctal sclerite straight also in ¾ ventrolateral view, while apex above angle forms a nearly half curve (Figs. 3b, 5a). Female genitalia (Fig. 2). Subgenital plate pale, as wide as 3/5 of the abdomen at its base; nearly three time wider than long. Rectangular or trapezoid with rounded corners, posterior margin straight or slightly convex, occasionally medially notched. Sternite VIII bears two large brown patches near the subgenital plate base, sternite IX bears paired dark brown spots laterally. Larva (Figs. 6-7). Pale with brown pattern. Pattern of head and pronotum consist of brown marks on yellow ground as illustrated (Fig. 6). Lacinia generotypic. Mediodorsal row of long, apically blunt hairs run from postfrontal suture to end of tergite 10; length of hairs one third of corresponding tergal length. Hind femur long and narrow, ratio of length and width is 4.5:1. Row of marginal swimming hairs complete on both femur and tibia, nearly as long as femur’s width and one and half longer than tibia’s width. Apical row of setae on tergites as long as one tenth of corresponding tergite; setae mixed with blunt hairs similar to those of the mediodorsal row, their length 2-4 times longer than setae. Sternite 8 with medially incomplete apical row of setae, row complete on sternites 9-10. Additional setae present on medial part of sternites 8-10 up to a quarter or half their length; these setae may be absent on sternites 8-9. Sternite also armed with long hairs curved outwards. Paraprocts lack distinct setation, but rarely with 1-2 strong setae (Fig. 7). Egg (Figs. 8-11, 16e). Typical of the Perlodini tribe, trilateral (Figs. 8-9, 16e). Length 400 µm, width 300 µm. Shape egg- like with cut-off base, wider than the 2/3 of its length (Fig. 8); triangular in apical view (Fig. 9). Collar lacking. Anchor with several rows of rounded, petal-like plates on the disc edge; globular bodies arranged in 3-4 peripheral rows just inside the petal-like plates, anchor surface wrinkled (Figs. 10-11). Chorion covered with tiny warts; ornamentation of polygonal FCIs distinct on operculum but hardly visible on basal part (Fig. 8-9). Micropyles placed in a transverse row around midlength, not raised (Fig. 8). Affinities . Size and head pattern of the new species is similar to Perlodes dispar . Both species lack the distinct, yellow C or V-shaped pattern by the M-line, characteristic for P. intricatus , P. jurassicus and P. mortoni . Females separate themselves from P. dispar and all the European species by their narrower subgenital plate. P. dispar male is micropterous while P. floridus is fully winged. Male paraprocts reminiscent of P. microcephalus in ventral view (Zwick 1997:494., Fig. 6a), but sclerite has a distinct apical angle in P. floridus but is gently curved in P. microcephalus . Paraproctal sclerites are also distinctly curved in ¾ ventrolateral view in comparison with the other European species: P. dispar , P. microcephalus (Marten 1991:400., Fig. 10), P. intricatus , P. jurassicus (Knispel et al. 2002:186., Figs. 1, 4). Identification of Perlodes larvae are not yet clear. Nevertheless, the larva of P. floridus seems to be distinctive by the setation of the three last sternites and the long, narrow hind femur. Egg of P. floridus is rather distinctive and can be easily separated from the known Perlodes species by its size, shape and anchor: Figs. 16 a-f; P. dispar, P. microcephalus (Kovács & Murányi 2008:117., Fig. 2a- b), P. frisonanus (Isobe 1988: 34., Fig. 9), P. intricatus , P. jurassicus (Knispel et al. 2002:187., Figs. 7, 10), P. mortoni (Zwick 2011:294., Fig. 5 mo-1-7). Etymology . The name floridus (from the Latin word flora , meaning flower) refers to the flower-like anchor of the egg that distinguishes the new species from the hitherto known Perlodes . Ecological notes . The specimens were found in different types of rivers between 35 and 975 m a.s.l. (Figs. 17-24). All of them have fast flow, even in the low altitudes, and stony substrate, but width varies between 5 to 20 meters. Larvae were found under big stones in the strongest current, life cycle seems to be one-year like in other Perlodes species. Adults occurred between 12th of March and 29th of May, and were captured on stones and vegetation close to the bank. Due to the wide range of its habitats, it was found together with many other stoneflies. The most species rich locality is the Sperchios River at Agios Georgios, where earlier (10.04.1967) the following species were found (Berthélemy 1971): Brachyptera beali , B. helenica , B. graeca , B. phthiotica (?), Brachyptera sp., Protonemura mattheyi , Amphinemura sperchiana , Leuctra sp., Perlodes microcephalus , Isoperla tripartita , Perla marginata , P. pallida , Chloroperla sp. ( tripunctata ?); in 2010 we found there the species Leuctra moreae , Protonemura intricata, Perla pallida . In the other rivers P. floridus shares the habitat with the further species: Brachyptera risi , Leuctra olympia , Capnia nigra , Amphinemura quadrangularis , Nemoura cinerea , Besdolus imhoffi , Isoperla grammatica , Perla abdominalis , Dinocras sp., Eoperla ochracea , Chloroperla tripunctata , Siphonoperla sp. Remarks : Berthélemy (1971) cited P. microcephalus from the Sperchios River where we found P. floridus only. Unfortunatelly, we had no access to study the specimens held in the Toulouse Museum, France. : Published as part of Kovács, Tibor, Vinçon, Gilles, Murányi, Dávid & Sivec, Ignac, 2012, A New Perlodes Species And Its Subspecies From The Balkan Peninsula (Plecoptera: Perlodidae), pp. 182-192 in Illiesia 8 (20) on pages 183-188, DOI: 10.5281/zenodo.4753269 : {"references": ["Zwick, P. 1997. Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. Pp. 489 - 496. In: Landolt, P. & M. Sartori (eds). Ephemeroptera & Plecoptera: Biology-Ecology- Systematics. MTL - Mauron + Tinguely & Lachat SA, Switzerland / Fribourg, 569 pp.", "Marten, M. 1991. The effect of temperature on the egg incubation period of Perlodes microcephalus, Perlodes dispar (Plecoptera: Perlodidae) and Perla burmeisteriana (Plecoptera: Perlidae). Pp. 387 - 401. In: Tercedor, J. A. & A. S. Ortega (eds). Overview and strategies of Ephemeroptera and Plecoptera. Sandhill Crane Press, Gainesville, Florida, 588 pp.", "Knispel, S., E. Rosciszewska, G. Vincon, & V. Lubini. 2002. The status of Perlodes jurassicus Aubert, 1946 (Insecta: Plecoptera: Perlodidae). Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 75: 183 - 189.", "Kovacs, T. & D. Muranyi. 2008. Data to the distribution of genus Perlodes in Hungary (Plecoptera: Perlodidae). Folia Historico-Naturalia Musei Matraensis, 32: 115 - 118.", "Isobe, Y. 1988. Eggs of Plecoptera from Japan. Biology of Inland Waters, 4: 27 - 39.", "Zwick, P. 2011. Polymorphism and taxonomic problems in the Perlodes microcephalus group (Plecoptera: Perlodidae); Perlodes mortoni removed from synonymy. Illiesia, 7 (26): 291 - 296.", "Berthelemy, C. 1971. Plecopteres de Grece Centrale et d'Eubee. Biologia Gallo- Hellenica, 3 (1) (1970): 23 - 56."]}
format Text
author Kovács, Tibor
Vinçon, Gilles
Murányi, Dávid
Sivec, Ignac
author_facet Kovács, Tibor
Vinçon, Gilles
Murányi, Dávid
Sivec, Ignac
author_sort Kovács, Tibor
title Perlodes floridus Kovacs & Vincon 2012, sp. n.
title_short Perlodes floridus Kovacs & Vincon 2012, sp. n.
title_full Perlodes floridus Kovacs & Vincon 2012, sp. n.
title_fullStr Perlodes floridus Kovacs & Vincon 2012, sp. n.
title_full_unstemmed Perlodes floridus Kovacs & Vincon 2012, sp. n.
title_sort perlodes floridus kovacs & vincon 2012, sp. n.
publisher Zenodo
publishDate 2012
url https://dx.doi.org/10.5281/zenodo.4758140
https://zenodo.org/record/4758140
long_lat ENVELOPE(-57.950,-57.950,-63.950,-63.950)
ENVELOPE(9.914,9.914,63.019,63.019)
ENVELOPE(-84.500,-84.500,-78.767,-78.767)
geographic Ortega
Stripe
Landolt
geographic_facet Ortega
Stripe
Landolt
genre Protonemura intricata
genre_facet Protonemura intricata
op_relation http://publication.plazi.org/id/2F53FF8DD20E1F136015FFA1FA7CFFD7
http://zoobank.org/71EF3B40-E042-4D86-987E-6E7B609AE5B1
https://zenodo.org/communities/biosyslit
https://dx.doi.org/10.5281/zenodo.4753269
http://publication.plazi.org/id/2F53FF8DD20E1F136015FFA1FA7CFFD7
https://dx.doi.org/10.5281/zenodo.4753273
https://dx.doi.org/10.5281/zenodo.4753279
https://dx.doi.org/10.5281/zenodo.4753283
https://dx.doi.org/10.5281/zenodo.4753275
https://dx.doi.org/10.5281/zenodo.4753285
http://zoobank.org/71EF3B40-E042-4D86-987E-6E7B609AE5B1
https://dx.doi.org/10.5281/zenodo.4758141
https://zenodo.org/communities/biosyslit
op_rights Open Access
Creative Commons Zero v1.0 Universal
https://creativecommons.org/publicdomain/zero/1.0/legalcode
cc0-1.0
info:eu-repo/semantics/openAccess
op_rightsnorm CC0
op_doi https://doi.org/10.5281/zenodo.4758140
https://doi.org/10.5281/zenodo.4753269
https://doi.org/10.5281/zenodo.4753273
https://doi.org/10.5281/zenodo.4753279
https://doi.org/10.5281/zenodo.4753283
https://doi.org/10.5281/zenodo.4753275
https://d
_version_ 1766174474341711872
spelling ftdatacite:10.5281/zenodo.4758140 2023-05-15T18:03:34+02:00 Perlodes floridus Kovacs & Vincon 2012, sp. n. Kovács, Tibor Vinçon, Gilles Murányi, Dávid Sivec, Ignac 2012 https://dx.doi.org/10.5281/zenodo.4758140 https://zenodo.org/record/4758140 unknown Zenodo http://publication.plazi.org/id/2F53FF8DD20E1F136015FFA1FA7CFFD7 http://zoobank.org/71EF3B40-E042-4D86-987E-6E7B609AE5B1 https://zenodo.org/communities/biosyslit https://dx.doi.org/10.5281/zenodo.4753269 http://publication.plazi.org/id/2F53FF8DD20E1F136015FFA1FA7CFFD7 https://dx.doi.org/10.5281/zenodo.4753273 https://dx.doi.org/10.5281/zenodo.4753279 https://dx.doi.org/10.5281/zenodo.4753283 https://dx.doi.org/10.5281/zenodo.4753275 https://dx.doi.org/10.5281/zenodo.4753285 http://zoobank.org/71EF3B40-E042-4D86-987E-6E7B609AE5B1 https://dx.doi.org/10.5281/zenodo.4758141 https://zenodo.org/communities/biosyslit Open Access Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 info:eu-repo/semantics/openAccess CC0 Biodiversity Taxonomy Animalia Arthropoda Insecta Plecoptera Perlodidae Perlodes Perlodes floridus article-journal ScholarlyArticle Text Taxonomic treatment 2012 ftdatacite https://doi.org/10.5281/zenodo.4758140 https://doi.org/10.5281/zenodo.4753269 https://doi.org/10.5281/zenodo.4753273 https://doi.org/10.5281/zenodo.4753279 https://doi.org/10.5281/zenodo.4753283 https://doi.org/10.5281/zenodo.4753275 https://d 2022-03-10T13:30:59Z Perlodes floridus Kovács & Vinçon sp. n. (Figs. 1-3 ab, 4a, 5a, 6-7, 8-11, 16e) Material examined. Holotype: MONTENEGRO, Podgorica municipality, Gornji Mileš, Rogamsko brdo, Cijevna River, N42°23’59.7”, E19°18’42.6”, 65 m a.s.l., 25.05.2009, 1♀ +O (Figs. 8-9, 11, 16e), KT-MG-UL, MM: 2009-49. Paratypes: same locality and date: 2 E, 1♂ (Figs. 3 ab), KT-MG-UL, MM: 2009-49; Dinoša, Cijevna River, N42°24’24.6”, E19°20’56.1”, 85 m, 29.05.2009, 1♂, 1♀ +O, (Figs. 1-2), KT-MG-UL, MM: 2009-66; 13.04.2010, 6L (Figs. 6-7), KT-MG-UL, MM: 2010-31. Zlatica, Morača River, N42°28’40.2”, E19°18’19.8”, 50 m, 2010.04.17., 3L, KT- MG-UL, MM: 2010-40. ALBANIA, Librazhd district, Qukës Shkumbin, Shkumbin River, N41°05’45.7”, E20°26’34.0”, 380 m, (2006/4), 09.04.2006, 1 E, EZ-FZ-HA-MD, HNHM: PLP 2184; Pukë district, Mertur, Mertur Stream at the influence to Koman Lake, N42°13’36.8”, E19°54’18.0”, 180 m, (2006/56), 15.04.2006, 1♀ +O, EZ-FZ-HA-MD, HNHM: PLP 2246; Sarandë district, Çikë Mts, Borsh, Borsh River N of the village, N40°03’57.1”, E19°50’53.5”, 35 m, (2008/18), 12.03.2008, 1E, 1♂, CSz-MD, HNHM: PLP 2525. GREECE, West Macedonia, Grevena regional unit, Aetia, Venetikos River, N40°04’27.9”, E21°12’06.0”, 973 m, (2006/114), 14.05.2006, 1♀ +O, DL-KJ-MD, HNHM: PLP 2135; Eleftherohori, Venetikos River, 24.04.1989, 1♀ +O, HB-SI, PMSL: 03220; N40°03’06.6”, E21°28’50.2”, 475 m, (2006/111), 13.05.2006, 1E, 1♀ +O, DL-KJ-MD, HNHM: PLP 2132; Kipourio, Venetikos River, 24.04.1989, 9E, 1♂, HB-SI, PMSL; Zakas, Venetikos River, N40°02’19.3”, E21°17’19.2”, 699 m, (2006/112), 14.05.2006, 1L, 1♂ (Figs. 4a, 5a), DL-KJ-MD, HNHM: PLP 2144; Thessaly, Trikala regional unit, Ambelia, Kalambaka, 20.04.1989, 9E, 6♂, 6♀, HB-SI (8E, 4♂, 4♀, O, PMSL: 03148; 1E, 2♂, 2♀, O (Fig. 10), CGV); Kastania, Kalambaka, 20.04.1989, 7E, 1♂, HB-SI, PMSL: 03141; Kato Palagokaria, 19.04.1989, 2♀ +O, HB-SI, PMSL: 03119; Longiai, 19.04.1989, 3♀ +O, (in very poor condition), HB-SI, PMSL: 03126; Karditsa regional unit, Sarantaporo, small river S of the village, N39°09’13.9”, E21°49’50.7”, 700 m, (2011/39), 08.05.2011, 1♂, KJ-MD-SzT-UZs, HNHM: PLP 3680; Central Greece, Phthiotis regional unit, Agios Georgios, Sperchios River W of the village, N38°57’00.5”, E21°56’42.7”, 365 m, (2011/35), 08.05.2011, 1♂, 1♀ +O, KJ-MD-SzT-UZs, HNHM: PLP 3669; Timfristos, Sperchios River, (207), 29.04.1989, 1♀ +O, HB-SI, PMSL; Phocis regional unit, Vardousia Mts, Stromi, 26.04.1990, 1E, 1♀ +O, HB-SI, PMSL: 03212; Giona Mts, Lefkaditi, NW of Amfissa, 15.04.1987, 4♂, 2♀, O, Drosopoulos, PMSL: 03300. Diagnosis . Head of the imago lacks yellow pattern by the M-line. Male macropterous, paraproct sclerite with long and straight apical part, apex angled. Female subgenital plate narrow. Larval hind femur long and narrow, last sternites with distinct setation. Egg with flower-like anchor, shape egg-like with cutoff base, FCIs distinct only on opercular part. Description (Figs. 1-2). Dark brown with distinct yellow markings. A small sized Perlodes . Body length: males 12.5-18 mm, females 14.5-20.5 mm; forewing length: males 11.5-14.5, females 15-19.5 mm. Dorsal part of head brown with two large pale patches (Fig. 1). One is a crown or tulip-shaped pattern on the occiput that laterally reaches the midline of the dark compound eyes, then deeply incised while central part pointed up to the postfrontal suture. Anterior pale patch is oval, delimited by the three, yellow ocelli. Occipital rugosities, tentorial callosities and M-line indistinct. Ventral part of head yellow, antennae and palpi brown. Pronotum brown with a median longitudinal yellow stripe (Fig. 1), prothorax ventrally yellow besides dark brown rhomboid or arrow-shaped dark patch anterior to furcasternum. Dorsal part of meso- and metathorax dark brown, ventrally mostly dark brownish. Legs all brown, wings normal sized, veins dark brown. Abdominal segments 1-4 divided by pleural membrane (as well as in larvae). Abdomen dorsally brown; males bear transverse pale patches on tergites IX–X, paired medial spots on tergite VIII, these may be present also on tergites VI–VII. Abdominal sternites brown, gradually darkened towards apex in males (transverse pale patches may be present medially); in females generally yellow while sternite I brown, sternites II-VII with a large anteromedial and variable lateral brown patches. Cerci brown in both sexes. Male genitalia (Figs. 3a, b, 4a, 5a). Tergite X undivided, not raised. Paraproctal sclerite in ventral view with long and straight apical part that gradually narrows towards its apex; sides parallel and keeled beneath the apex that is strongly angled (120°) and sharply pointed (Figs. 3a, 4a). Apical part of paraproctal sclerite straight also in ¾ ventrolateral view, while apex above angle forms a nearly half curve (Figs. 3b, 5a). Female genitalia (Fig. 2). Subgenital plate pale, as wide as 3/5 of the abdomen at its base; nearly three time wider than long. Rectangular or trapezoid with rounded corners, posterior margin straight or slightly convex, occasionally medially notched. Sternite VIII bears two large brown patches near the subgenital plate base, sternite IX bears paired dark brown spots laterally. Larva (Figs. 6-7). Pale with brown pattern. Pattern of head and pronotum consist of brown marks on yellow ground as illustrated (Fig. 6). Lacinia generotypic. Mediodorsal row of long, apically blunt hairs run from postfrontal suture to end of tergite 10; length of hairs one third of corresponding tergal length. Hind femur long and narrow, ratio of length and width is 4.5:1. Row of marginal swimming hairs complete on both femur and tibia, nearly as long as femur’s width and one and half longer than tibia’s width. Apical row of setae on tergites as long as one tenth of corresponding tergite; setae mixed with blunt hairs similar to those of the mediodorsal row, their length 2-4 times longer than setae. Sternite 8 with medially incomplete apical row of setae, row complete on sternites 9-10. Additional setae present on medial part of sternites 8-10 up to a quarter or half their length; these setae may be absent on sternites 8-9. Sternite also armed with long hairs curved outwards. Paraprocts lack distinct setation, but rarely with 1-2 strong setae (Fig. 7). Egg (Figs. 8-11, 16e). Typical of the Perlodini tribe, trilateral (Figs. 8-9, 16e). Length 400 µm, width 300 µm. Shape egg- like with cut-off base, wider than the 2/3 of its length (Fig. 8); triangular in apical view (Fig. 9). Collar lacking. Anchor with several rows of rounded, petal-like plates on the disc edge; globular bodies arranged in 3-4 peripheral rows just inside the petal-like plates, anchor surface wrinkled (Figs. 10-11). Chorion covered with tiny warts; ornamentation of polygonal FCIs distinct on operculum but hardly visible on basal part (Fig. 8-9). Micropyles placed in a transverse row around midlength, not raised (Fig. 8). Affinities . Size and head pattern of the new species is similar to Perlodes dispar . Both species lack the distinct, yellow C or V-shaped pattern by the M-line, characteristic for P. intricatus , P. jurassicus and P. mortoni . Females separate themselves from P. dispar and all the European species by their narrower subgenital plate. P. dispar male is micropterous while P. floridus is fully winged. Male paraprocts reminiscent of P. microcephalus in ventral view (Zwick 1997:494., Fig. 6a), but sclerite has a distinct apical angle in P. floridus but is gently curved in P. microcephalus . Paraproctal sclerites are also distinctly curved in ¾ ventrolateral view in comparison with the other European species: P. dispar , P. microcephalus (Marten 1991:400., Fig. 10), P. intricatus , P. jurassicus (Knispel et al. 2002:186., Figs. 1, 4). Identification of Perlodes larvae are not yet clear. Nevertheless, the larva of P. floridus seems to be distinctive by the setation of the three last sternites and the long, narrow hind femur. Egg of P. floridus is rather distinctive and can be easily separated from the known Perlodes species by its size, shape and anchor: Figs. 16 a-f; P. dispar, P. microcephalus (Kovács & Murányi 2008:117., Fig. 2a- b), P. frisonanus (Isobe 1988: 34., Fig. 9), P. intricatus , P. jurassicus (Knispel et al. 2002:187., Figs. 7, 10), P. mortoni (Zwick 2011:294., Fig. 5 mo-1-7). Etymology . The name floridus (from the Latin word flora , meaning flower) refers to the flower-like anchor of the egg that distinguishes the new species from the hitherto known Perlodes . Ecological notes . The specimens were found in different types of rivers between 35 and 975 m a.s.l. (Figs. 17-24). All of them have fast flow, even in the low altitudes, and stony substrate, but width varies between 5 to 20 meters. Larvae were found under big stones in the strongest current, life cycle seems to be one-year like in other Perlodes species. Adults occurred between 12th of March and 29th of May, and were captured on stones and vegetation close to the bank. Due to the wide range of its habitats, it was found together with many other stoneflies. The most species rich locality is the Sperchios River at Agios Georgios, where earlier (10.04.1967) the following species were found (Berthélemy 1971): Brachyptera beali , B. helenica , B. graeca , B. phthiotica (?), Brachyptera sp., Protonemura mattheyi , Amphinemura sperchiana , Leuctra sp., Perlodes microcephalus , Isoperla tripartita , Perla marginata , P. pallida , Chloroperla sp. ( tripunctata ?); in 2010 we found there the species Leuctra moreae , Protonemura intricata, Perla pallida . In the other rivers P. floridus shares the habitat with the further species: Brachyptera risi , Leuctra olympia , Capnia nigra , Amphinemura quadrangularis , Nemoura cinerea , Besdolus imhoffi , Isoperla grammatica , Perla abdominalis , Dinocras sp., Eoperla ochracea , Chloroperla tripunctata , Siphonoperla sp. Remarks : Berthélemy (1971) cited P. microcephalus from the Sperchios River where we found P. floridus only. Unfortunatelly, we had no access to study the specimens held in the Toulouse Museum, France. : Published as part of Kovács, Tibor, Vinçon, Gilles, Murányi, Dávid & Sivec, Ignac, 2012, A New Perlodes Species And Its Subspecies From The Balkan Peninsula (Plecoptera: Perlodidae), pp. 182-192 in Illiesia 8 (20) on pages 183-188, DOI: 10.5281/zenodo.4753269 : {"references": ["Zwick, P. 1997. Rauserella, a new genus of Plecoptera (Perlodidae), with notes on related genera. Pp. 489 - 496. In: Landolt, P. & M. Sartori (eds). Ephemeroptera & Plecoptera: Biology-Ecology- Systematics. MTL - Mauron + Tinguely & Lachat SA, Switzerland / Fribourg, 569 pp.", "Marten, M. 1991. The effect of temperature on the egg incubation period of Perlodes microcephalus, Perlodes dispar (Plecoptera: Perlodidae) and Perla burmeisteriana (Plecoptera: Perlidae). Pp. 387 - 401. In: Tercedor, J. A. & A. S. Ortega (eds). Overview and strategies of Ephemeroptera and Plecoptera. Sandhill Crane Press, Gainesville, Florida, 588 pp.", "Knispel, S., E. Rosciszewska, G. Vincon, & V. Lubini. 2002. The status of Perlodes jurassicus Aubert, 1946 (Insecta: Plecoptera: Perlodidae). Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 75: 183 - 189.", "Kovacs, T. & D. Muranyi. 2008. Data to the distribution of genus Perlodes in Hungary (Plecoptera: Perlodidae). Folia Historico-Naturalia Musei Matraensis, 32: 115 - 118.", "Isobe, Y. 1988. Eggs of Plecoptera from Japan. Biology of Inland Waters, 4: 27 - 39.", "Zwick, P. 2011. Polymorphism and taxonomic problems in the Perlodes microcephalus group (Plecoptera: Perlodidae); Perlodes mortoni removed from synonymy. Illiesia, 7 (26): 291 - 296.", "Berthelemy, C. 1971. Plecopteres de Grece Centrale et d'Eubee. Biologia Gallo- Hellenica, 3 (1) (1970): 23 - 56."]} Text Protonemura intricata DataCite Metadata Store (German National Library of Science and Technology) Ortega ENVELOPE(-57.950,-57.950,-63.950,-63.950) Stripe ENVELOPE(9.914,9.914,63.019,63.019) Landolt ENVELOPE(-84.500,-84.500,-78.767,-78.767)