Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions
Nowadays, deaths from neoplasms are at the top of the World Health Organization list. The interest of scientists is focused on the search for natural substrates perspective for the targeted anticancer chemotherapy. Antarctic yeasts represent an unexplored object regarding their antineoplastic potent...
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ftdatacite:10.5281/zenodo.4675666 2023-05-15T13:57:43+02:00 Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions Rusinova-Videva Snezhana Hristova Dilyana Zaharieva Maya Nachkova Stefka Kambourova Margarita Najdenski Hristo Konstantinov Spiro 2021 https://dx.doi.org/10.5281/zenodo.4675666 https://zenodo.org/record/4675666 unknown Zenodo https://zenodo.org/communities/balkanbio2021 https://dx.doi.org/10.5281/zenodo.4675665 https://zenodo.org/communities/balkanbio2021 Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 info:eu-repo/semantics/openAccess CC-BY Sporobolomyces salmonicolor extracts, bioreactor, in vitro antiproliferative effect, apoptosis Text Poster article-journal ScholarlyArticle 2021 ftdatacite https://doi.org/10.5281/zenodo.4675666 https://doi.org/10.5281/zenodo.4675665 2021-11-05T12:55:41Z Nowadays, deaths from neoplasms are at the top of the World Health Organization list. The interest of scientists is focused on the search for natural substrates perspective for the targeted anticancer chemotherapy. Antarctic yeasts represent an unexplored object regarding their antineoplastic potential. The studies in the present work demonstrated the capacity of Sporobolomyces salmonicolor AL 36 for cell growth in different cultivation processes. During the transfer of small-scale flasks cultivation to the bioreactor system, optimal biomass quantities of approximately 6.0 g/L were recorded. A comparative examination of the metabolic profiles of the two extracts tested reveals differences in the synthesized molecules corresponding to different cytotoxicity (antineoplastic activity) in vitro on malignant cell lines. The median inhibitory concentration (IC 50 ) of each extract determined by the MTT test was used as a parameter for evaluating the antiproliferative effects. Most sensitive to the in vitro effect of the extract of Sp . salmonicolor AL 36 cultivated in flasks was the cell line SKW-3 (T cell leukemia, derivative of KE-37) - IC 50 = 35.3 µg/ml, while the bioreactor biomass extract was most cytotoxic for RPMI-8226 (multiple myeloma) cells - IC 50 = 28.27µg/ml. The proteome analysis of treated and untreated malignant cells showed that both yeast extracts have a strong potential to inhibit anti-apoptotic proteins which reveals a mode of action related to induction of apoptosis and proliferation inhibition. Still Image Antarc* Antarctic DataCite Metadata Store (German National Library of Science and Technology) Antarctic |
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DataCite Metadata Store (German National Library of Science and Technology) |
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language |
unknown |
topic |
Sporobolomyces salmonicolor extracts, bioreactor, in vitro antiproliferative effect, apoptosis |
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Sporobolomyces salmonicolor extracts, bioreactor, in vitro antiproliferative effect, apoptosis Rusinova-Videva Snezhana Hristova Dilyana Zaharieva Maya Nachkova Stefka Kambourova Margarita Najdenski Hristo Konstantinov Spiro Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions |
topic_facet |
Sporobolomyces salmonicolor extracts, bioreactor, in vitro antiproliferative effect, apoptosis |
description |
Nowadays, deaths from neoplasms are at the top of the World Health Organization list. The interest of scientists is focused on the search for natural substrates perspective for the targeted anticancer chemotherapy. Antarctic yeasts represent an unexplored object regarding their antineoplastic potential. The studies in the present work demonstrated the capacity of Sporobolomyces salmonicolor AL 36 for cell growth in different cultivation processes. During the transfer of small-scale flasks cultivation to the bioreactor system, optimal biomass quantities of approximately 6.0 g/L were recorded. A comparative examination of the metabolic profiles of the two extracts tested reveals differences in the synthesized molecules corresponding to different cytotoxicity (antineoplastic activity) in vitro on malignant cell lines. The median inhibitory concentration (IC 50 ) of each extract determined by the MTT test was used as a parameter for evaluating the antiproliferative effects. Most sensitive to the in vitro effect of the extract of Sp . salmonicolor AL 36 cultivated in flasks was the cell line SKW-3 (T cell leukemia, derivative of KE-37) - IC 50 = 35.3 µg/ml, while the bioreactor biomass extract was most cytotoxic for RPMI-8226 (multiple myeloma) cells - IC 50 = 28.27µg/ml. The proteome analysis of treated and untreated malignant cells showed that both yeast extracts have a strong potential to inhibit anti-apoptotic proteins which reveals a mode of action related to induction of apoptosis and proliferation inhibition. |
format |
Still Image |
author |
Rusinova-Videva Snezhana Hristova Dilyana Zaharieva Maya Nachkova Stefka Kambourova Margarita Najdenski Hristo Konstantinov Spiro |
author_facet |
Rusinova-Videva Snezhana Hristova Dilyana Zaharieva Maya Nachkova Stefka Kambourova Margarita Najdenski Hristo Konstantinov Spiro |
author_sort |
Rusinova-Videva Snezhana |
title |
Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions |
title_short |
Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions |
title_full |
Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions |
title_fullStr |
Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions |
title_full_unstemmed |
Evaluation of the antineoplastic activity of Antarctic yeast Sporobolomyces salmonicolor grown at different culture conditions |
title_sort |
evaluation of the antineoplastic activity of antarctic yeast sporobolomyces salmonicolor grown at different culture conditions |
publisher |
Zenodo |
publishDate |
2021 |
url |
https://dx.doi.org/10.5281/zenodo.4675666 https://zenodo.org/record/4675666 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://zenodo.org/communities/balkanbio2021 https://dx.doi.org/10.5281/zenodo.4675665 https://zenodo.org/communities/balkanbio2021 |
op_rights |
Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5281/zenodo.4675666 https://doi.org/10.5281/zenodo.4675665 |
_version_ |
1766265542518243328 |