Impact of short- and long-term exposure to elevated seawater pCO2 on metabolic rate and hypoxia tolerance in Octopus rubescens

• Main Authors: Onthank, Kirt, Trueblood, Lloyd, Schrock-Duff, Taylir, Kore, Lydia
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Zenodo 2021
• Subjects: Ocean acidification
• Online Access: https://dx.doi.org/10.5281/zenodo.4641238; https://zenodo.org/record/4641238

Much of the CO 2 released by human activity into the atmosphere is dissolving into the oceans making them more acidic. In this study we provide the first data on the short- and long-term impacts of ocean acidification on octopuses. We measured routine metabolic rate (RMR) and critical oxygen pressure (P crit ) of Octopus rubescens at elevated CO 2 pressure (pCO 2 ) with no prior acclimation, one, and five weeks acclimation. Octopuses showed significantly higher RMRs in 1500 μatm pCO 2 environments with no prior acclimation than octopuses in 700 μatm or 360 μatm environments. However, both one and five weeks of acclimation showed no significant difference in RMRs between octopuses at differing pCO 2 , indicating that octopuses acclimated rapidly to elevated pCO 2 . In octopuses acclimated for five weeks at 1500 μatm pCO 2 we observed impaired hypoxia tolerance as demonstrated by a significantly higher critical oxygen pressure than those acclimated to 700 μatm pCO 2 . Our findings suggest that O. rubescens experiences short-term stress in elevated pCO 2 , but is able to acclimate over time. However, while this species may be able to acclimate to near-term ocean acidification, compounding environmental effects of acidification and hypoxia may present a physiological challenge for this species. : This data provides the analysis and datasets to replicate the statistical analyses and figures that appear in the paper "Impact of short- and long-term exposure to elevated seawater pCO2 on metabolic rate and hypoxia tolerance in Octopus rubescens", as well as some additional figures that more fully explain analyses such as semivariograms and test statistic distributions. To run critical oxygen pressure measurements analyses you will need the raw data contained in "Pcrit_data.zip" and the code contained in "Pcrit_Analysis.pdf". This will write a .csv file of Pcrit values that will be used the further analysis. For the remainder of the analyses, you will need the code contained in "Octopus_hydpercapnia.pdf" or alternatively in "Octopus_hypercapnia.Rmd", and you will need the data contained in "GPS_waypoints.zip", "Seawater_ph_2015.csv", "treatment_carbonate2014.csv", "Short_Term_RMR_2014.csv", "Long_Term_RMR_2014.csv", and "Pcrit_2014.csv". Funding provided by: M.J. Murdock Charitable Trust Award Number: 2013260:MNL:2/27/2014