Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2...
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ftdatacite:10.5281/zenodo.4549139 2023-05-15T14:02:09+02:00 Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus Gerdol, M. Lucente, D. Buonocore, F. Poerio, E. Scapigliati, G. Mattiucci, S. Pallavicini, A. Cimmaruta R 2019 https://dx.doi.org/10.5281/zenodo.4549139 https://zenodo.org/record/4549139 unknown Zenodo https://zenodo.org/communities/unitus https://dx.doi.org/10.5281/zenodo.4549138 https://zenodo.org/communities/unitus Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 info:eu-repo/semantics/openAccess CC-BY Text Journal article article-journal ScholarlyArticle 2019 ftdatacite https://doi.org/10.5281/zenodo.4549139 https://doi.org/10.5281/zenodo.4549138 2021-11-05T12:55:41Z This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene. Text Antarc* Antarctic Icefish DataCite Metadata Store (German National Library of Science and Technology) Antarctic The Antarctic |
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DataCite Metadata Store (German National Library of Science and Technology) |
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description |
This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene. |
format |
Text |
author |
Gerdol, M. Lucente, D. Buonocore, F. Poerio, E. Scapigliati, G. Mattiucci, S. Pallavicini, A. Cimmaruta R |
spellingShingle |
Gerdol, M. Lucente, D. Buonocore, F. Poerio, E. Scapigliati, G. Mattiucci, S. Pallavicini, A. Cimmaruta R Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
author_facet |
Gerdol, M. Lucente, D. Buonocore, F. Poerio, E. Scapigliati, G. Mattiucci, S. Pallavicini, A. Cimmaruta R |
author_sort |
Gerdol, M. |
title |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_short |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_full |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_fullStr |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_full_unstemmed |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_sort |
molecular and structural characterization of mhc class ii β genes reveals high diversity in the cold-adapted icefish chionodraco hamatus |
publisher |
Zenodo |
publishDate |
2019 |
url |
https://dx.doi.org/10.5281/zenodo.4549139 https://zenodo.org/record/4549139 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_relation |
https://zenodo.org/communities/unitus https://dx.doi.org/10.5281/zenodo.4549138 https://zenodo.org/communities/unitus |
op_rights |
Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5281/zenodo.4549139 https://doi.org/10.5281/zenodo.4549138 |
_version_ |
1766272241498062848 |