Centennial clonal stability of asexual Daphnia in Greenland lakes despite climate variability

• Main Authors: Dane, Maison, Anderson, N. John, Osburn, Chistopher L., Colbourne, John K., Frisch, Dagmar
• Format: Dataset
• Language: unknown
• Published: Zenodo 2020
• Subjects: Greenland; Kangerlussuaq; Morton; Lynch; Dane; Appleby
• Online Access: https://dx.doi.org/10.5281/zenodo.4118370; https://zenodo.org/record/4118370

Daphnia_microsatellite_data_Dane_etal.2020.csv: Microsatellite genotypes from three study lakes (SS4, SS1381, and SS1590) in the Kangerlussuaq area, West Greenland. Microsatellite loci were amplified in single, 12.5 µl multiplex reactions (Type-it PCR kit, Qiagen Inc, Valencia, CA, USA), using an Eppendorf Nexus Thermal Cycler with thermal cycle conditions recommended in the Type-it PCR kit manual. Ten microsatellite primers representing genome-wide loci were used for genotyping; details in (Colbourne et al. 2004; Frisch et al., 2014). Two primers (Dp90, Dp377) failed to amplify in a consistent manner and were therefore excluded from further analysis. Amplified microsatellites were genotyped on an Applied Biosystems 3730 genetic analyser. We used the microsatellite plugin for Geneious 7.0.6 (https://www.geneious.com) for peak calling and binning. Called peaks were visually inspected and manually adjusted when necessary. SS4_sediment.core_data_Fig2_Dane_et_al2020.xlsx : Information on various parameters of sediment cores collected in Lake SS4, Kangerlussuq area, West Greenland. Data used in Dane et al. 2020, Figure 2 (panels B and C) are derived from two sediment cores: one for fluorescence (section at 0.5 cm intervals, Depth ) and one for Daphnia ephippia analyses (1-cm intervals). Percentage organic matter content (loss-on-ignition at 550 °C, OM% ) was used to correlate the two cores to each other and to a previously-dated sediment core (see Dane et al. 2020, Methods). The fluorescence derived parameter Parafac component C2 was used as an indicator of the abundance of purple sulphur bacteria. The organic carbon burial rate ( OC AR , g C m–2 yr–1) was also calculated for this core (see Anderson et al. 2019). The Daphnia core was used for the microsatellite analyses and the accumulation rate of ephippia ( ephippia AR ) at the core site was estimated. For further details please see associated publication in Ecology and Evolution. : {"references": ["Colbourne, J. K., Robison, B., Bogart, K., & Lynch, M. (2004). Five hundred and twenty-eight microsatellite markers for ecological genomic investigations using Daphnia. Molecular Ecology Notes, 4(3), 485-490.", "Frisch, D., Morton, P. K., Roy Chowdhury, P., Culver, B. W., Colbourne, J. K., Weider, L. J., & Jeyasingh, P. D. (2014). A millennial-scale chronicle of evolutionary responses to cultural eutrophication in Daphnia. Ecology Letters, 17(3), 360\u2013368.", "Anderson, N.J., Appleby, P.G., Bindler, R., Renberg, I., Conley, D.J., Fritz, S.C., . . . Yang, H. (2019). Landscape-Scale Variability of Organic Carbon Burial by SW Greenland Lakes. Ecosystems, 22, 1706\u20131720"]}