Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam
Samuel J. Gurr 1 *, Shelly A. Trigg 3 , Brent Vadopalas 2 , Steven B. Roberts 3 , Hollie M. Putnam 1 1 University of Rhode Island, College of the Environment and Life Sciences, 120 Flagg Rd, Kingston, RI 02881 USA 2 University of Washington, Washington Sea Grant, 3716 Brooklyn Ave NE, Seattle, WA 98...
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ftdatacite:10.5281/zenodo.3903019 2023-05-15T17:52:13+02:00 Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam Gurr, Samuel Trigg, Shelly A. Vadopalas, Brent Roberts, Steven B. Putnam, Hollie M. 2020 https://dx.doi.org/10.5281/zenodo.3903019 https://zenodo.org/record/3903019 unknown Zenodo https://github.com/SamGurr/Intragenerational_thresholds_OA/releases/tag/20191219_version_release https://github.com/SamGurr/Intragenerational_thresholds_OA/releases/tag/20191219_version_release https://dx.doi.org/10.5281/zenodo.3903018 Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode CC-BY-4.0 info:eu-repo/semantics/openAccess CC-BY stress acclimation, ocean acidification, oxidative stress, hormesis, mitochondrial response Text Journal article article-journal ScholarlyArticle 2020 ftdatacite https://doi.org/10.5281/zenodo.3903019 https://doi.org/10.5281/zenodo.3903018 2021-11-05T12:55:41Z Samuel J. Gurr 1 *, Shelly A. Trigg 3 , Brent Vadopalas 2 , Steven B. Roberts 3 , Hollie M. Putnam 1 1 University of Rhode Island, College of the Environment and Life Sciences, 120 Flagg Rd, Kingston, RI 02881 USA 2 University of Washington, Washington Sea Grant, 3716 Brooklyn Ave NE, Seattle, WA 98105 USA 3 University of Washington, School of Aquatic and Fishery Sciences, 1122 NE Boat St, Seattle, WA 98105 USA Moderate oxidative stress is a hypothesized driver of enhanced adaptive capacity and lifespan. Whereas thermal stress, irradiance, and dietary restriction show evidence of dose-dependent benefits for terrestrial vertebrates, the importance of stress acclimation remains understudied for establishment of enhanced stress response in marine ectotherms. Marine invertebrates of commercial and ecological importance are threatened by elevated p CO 2 and aragonite undersaturation. Stress acclimation may drive phenotypic variation advantageous for sustainable aquaculture of long-lived mollusc species such as the Pacific geoduck Panopea generosa . To test for lifestage and stress-intensity dependence in eliciting enhanced tolerance under subsequent stress encounters, we acclimated ~30 day old pediveliger geoduck larvae for 110 days to 1) an initial exposure of ambient and moderately elevated p CO 2 (920 µatm >1 Ω and 2800 µatm 0.4 Ω, respectively). We then exposed them to 2) an acute 7-day secondary exposure to ambient, moderate, and severely elevated p CO 2 conditions (750 µatm >1 Ω, 2800 µatm 0.4 Ω, and 4900 µatm 0.2 Ω, respectively). Clams were returned to ambient p CO 2 for 7 days and then subject to 3) a 7-day tertiary exposure to ambient and moderately elevated p CO 2 conditions (970 µatm >1 Ω and 3000 µatm 0.4 Ω, respectively) in a modified-reciprocal design. Throughout each exposure, we measured metrics of physiological performance (respiration rate, shell growth, total protein and organic biomass) and cellular stress response (total antioxidant capacity). Initial exposure to moderate pCO2 stress followed by secondary and tertiary exposure to moderate and severe pCO2 stress increased respiration rate, organic biomass, and shell size suggesting a stress-intensity-dependent effect of stress acclimation on energetics. Additionally, clams acclimated to moderate p CO 2 stress during initial exposure had lower antioxidant capacity at the termination of the experiment compared to clams under ambient conditions, supporting the hypothesis that stress over postlarval-juvenile development affects energy metabolism and oxidative status later in life. Time series and stress intensity-specific approaches can reveal life-stages and magnitudes of exposure, respectively, that may lead to beneficial phenotypes for commercial production of this long-lived clam. Text Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) Brooklyn ENVELOPE(-62.083,-62.083,-64.650,-64.650) Pacific |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
stress acclimation, ocean acidification, oxidative stress, hormesis, mitochondrial response |
spellingShingle |
stress acclimation, ocean acidification, oxidative stress, hormesis, mitochondrial response Gurr, Samuel Trigg, Shelly A. Vadopalas, Brent Roberts, Steven B. Putnam, Hollie M. Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam |
topic_facet |
stress acclimation, ocean acidification, oxidative stress, hormesis, mitochondrial response |
description |
Samuel J. Gurr 1 *, Shelly A. Trigg 3 , Brent Vadopalas 2 , Steven B. Roberts 3 , Hollie M. Putnam 1 1 University of Rhode Island, College of the Environment and Life Sciences, 120 Flagg Rd, Kingston, RI 02881 USA 2 University of Washington, Washington Sea Grant, 3716 Brooklyn Ave NE, Seattle, WA 98105 USA 3 University of Washington, School of Aquatic and Fishery Sciences, 1122 NE Boat St, Seattle, WA 98105 USA Moderate oxidative stress is a hypothesized driver of enhanced adaptive capacity and lifespan. Whereas thermal stress, irradiance, and dietary restriction show evidence of dose-dependent benefits for terrestrial vertebrates, the importance of stress acclimation remains understudied for establishment of enhanced stress response in marine ectotherms. Marine invertebrates of commercial and ecological importance are threatened by elevated p CO 2 and aragonite undersaturation. Stress acclimation may drive phenotypic variation advantageous for sustainable aquaculture of long-lived mollusc species such as the Pacific geoduck Panopea generosa . To test for lifestage and stress-intensity dependence in eliciting enhanced tolerance under subsequent stress encounters, we acclimated ~30 day old pediveliger geoduck larvae for 110 days to 1) an initial exposure of ambient and moderately elevated p CO 2 (920 µatm >1 Ω and 2800 µatm 0.4 Ω, respectively). We then exposed them to 2) an acute 7-day secondary exposure to ambient, moderate, and severely elevated p CO 2 conditions (750 µatm >1 Ω, 2800 µatm 0.4 Ω, and 4900 µatm 0.2 Ω, respectively). Clams were returned to ambient p CO 2 for 7 days and then subject to 3) a 7-day tertiary exposure to ambient and moderately elevated p CO 2 conditions (970 µatm >1 Ω and 3000 µatm 0.4 Ω, respectively) in a modified-reciprocal design. Throughout each exposure, we measured metrics of physiological performance (respiration rate, shell growth, total protein and organic biomass) and cellular stress response (total antioxidant capacity). Initial exposure to moderate pCO2 stress followed by secondary and tertiary exposure to moderate and severe pCO2 stress increased respiration rate, organic biomass, and shell size suggesting a stress-intensity-dependent effect of stress acclimation on energetics. Additionally, clams acclimated to moderate p CO 2 stress during initial exposure had lower antioxidant capacity at the termination of the experiment compared to clams under ambient conditions, supporting the hypothesis that stress over postlarval-juvenile development affects energy metabolism and oxidative status later in life. Time series and stress intensity-specific approaches can reveal life-stages and magnitudes of exposure, respectively, that may lead to beneficial phenotypes for commercial production of this long-lived clam. |
format |
Text |
author |
Gurr, Samuel Trigg, Shelly A. Vadopalas, Brent Roberts, Steven B. Putnam, Hollie M. |
author_facet |
Gurr, Samuel Trigg, Shelly A. Vadopalas, Brent Roberts, Steven B. Putnam, Hollie M. |
author_sort |
Gurr, Samuel |
title |
Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam |
title_short |
Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam |
title_full |
Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam |
title_fullStr |
Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam |
title_full_unstemmed |
Repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam |
title_sort |
repeat exposure to hypercapnic seawater modifies performance and oxidative status in a tolerant burrowing clam |
publisher |
Zenodo |
publishDate |
2020 |
url |
https://dx.doi.org/10.5281/zenodo.3903019 https://zenodo.org/record/3903019 |
long_lat |
ENVELOPE(-62.083,-62.083,-64.650,-64.650) |
geographic |
Brooklyn Pacific |
geographic_facet |
Brooklyn Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://github.com/SamGurr/Intragenerational_thresholds_OA/releases/tag/20191219_version_release https://github.com/SamGurr/Intragenerational_thresholds_OA/releases/tag/20191219_version_release https://dx.doi.org/10.5281/zenodo.3903018 |
op_rights |
Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode CC-BY-4.0 info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5281/zenodo.3903019 https://doi.org/10.5281/zenodo.3903018 |
_version_ |
1766159602558173184 |