Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology
Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be ma...
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ftdatacite:10.5281/zenodo.2826019 2023-05-15T13:57:47+02:00 Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin 2014 https://dx.doi.org/10.5281/zenodo.2826019 https://zenodo.org/record/2826019 en eng Zenodo https://zenodo.org/communities/hsr https://dx.doi.org/10.5281/zenodo.2826020 https://zenodo.org/communities/hsr Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 info:eu-repo/semantics/openAccess CC-BY Text Journal article article-journal ScholarlyArticle 2014 ftdatacite https://doi.org/10.5281/zenodo.2826019 https://doi.org/10.5281/zenodo.2826020 2021-11-05T12:55:41Z Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be made by producing of more lipophilic derivatives. In this study, a preliminary investigation of lipase-catalyzed trans-esterification reaction of ethyl ferulate and olive oil was investigated. The reaction was catalyzed by immobilized lipase from Candida antarctica (Novozym 435), to produce ferulate ester, a sunscreen agent. A statistical approach of Response surface methodology (RSM) was used to evaluate the interactive effects of reaction temperature (40-80°C), reaction time (4-12 hours), and amount of enzyme (0.1-0.5 g). The optimum conditions derived via RSM were reaction temperature 60°C, reaction time 2.34 hours, and amount of enzyme 0.3 g. The actual experimental yield was 59.6% ferulate ester under optimum condition, which compared well to the maximum predicted value of 58.0%. Text Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be made by producing of more lipophilic derivatives. In this study, a preliminary investigation of lipase-catalyzed trans-esterification reaction of ethyl ferulate and olive oil was investigated. The reaction was catalyzed by immobilized lipase from Candida antarctica (Novozym 435), to produce ferulate ester, a sunscreen agent. A statistical approach of Response surface methodology (RSM) was used to evaluate the interactive effects of reaction temperature (40-80°C), reaction time (4-12 hours), and amount of enzyme (0.1-0.5 g). The optimum conditions derived via RSM were reaction temperature 60°C, reaction time 2.34 hours, and amount of enzyme 0.3 g. The actual experimental yield was 59.6% ferulate ester under optimum condition, which compared well to the maximum predicted value of 58.0%. |
format |
Text |
author |
S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin |
spellingShingle |
S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
author_facet |
S. Mat Radzi N. J. Abd Rahman H. Mohd Noor N. Ariffin |
author_sort |
S. Mat Radzi |
title |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_short |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_full |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_fullStr |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_full_unstemmed |
Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology |
title_sort |
enzymatic synthesis of olive-based ferulate esters: optimization by response surface methodology |
publisher |
Zenodo |
publishDate |
2014 |
url |
https://dx.doi.org/10.5281/zenodo.2826019 https://zenodo.org/record/2826019 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://zenodo.org/communities/hsr https://dx.doi.org/10.5281/zenodo.2826020 https://zenodo.org/communities/hsr |
op_rights |
Open Access Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5281/zenodo.2826019 https://doi.org/10.5281/zenodo.2826020 |
_version_ |
1766265670597607424 |