Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity ...

• Main Authors: Šimić, Ivana, Zorec, Tomaž Mark, Lojkić, Ivana, Krešić, Nina, Poljak, Mario, Cliquet, Florence, Picard-Meyer, Evelyne, Wasniewski, Marine, Zrnčić, Vida, Ćukušić, Anđela, Bedeković, Tomislav
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Zenodo 2020
• Subjects: Biodiversity; Mammalia; Chiroptera; Chordata; Animalia; bats; bat; Guano; Myotis nattereri
• Online Access: https://dx.doi.org/10.5281/zenodo.13491343; https://zenodo.org/doi/10.5281/zenodo.13491343

(Uploaded by Plazi for the Bat Literature Project) To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus ...