A rapid PCR-based test for identification of fifteen British bat species ...
(Uploaded by Plazi for the Bat Literature Project) The development of a rapid method for identification of the fifteen common species of British bat is described: Barbastella barbastellus, Eptesicus serotinus, Myotis bechsteinii, Myotis daubentonii, Myotis mystacinus, Myotis nattereri, Nyctalus leis...
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Online Access: | https://dx.doi.org/10.5281/zenodo.13467174 https://zenodo.org/doi/10.5281/zenodo.13467174 |
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ftdatacite:10.5281/zenodo.13467174 2024-09-15T17:57:36+00:00 A rapid PCR-based test for identification of fifteen British bat species ... Hamilton, P. B. Uren Webster, T. M. Basiewicz, M. Kennedy, E. V. De-Bastos, E. S. R. Mathews, F. 2015 https://dx.doi.org/10.5281/zenodo.13467174 https://zenodo.org/doi/10.5281/zenodo.13467174 unknown Zenodo hash://md5/938e44d642e1fd794c6c4fcdd7af2451 hash://sha256/ce5421f4b2f306aa7a726bbf7c64ebfc1295e4002c5ae3527f387f44bfa622f5 zotero://select/groups/5435545/items/B5BL8UVM https://zotero.org/groups/5435545/items/B5BL8UVM https://linker.bio/cut:hash://md5/3c24d4cbaf3a45dea1f0debaf29daaf6!/b173052-175521 hash://md5/26f7ce5dd404e33c6570edd4ba250d20 hash://md5/938e44d642e1fd794c6c4fcdd7af2451 hash://sha256/ce5421f4b2f306aa7a726bbf7c64ebfc1295e4002c5ae3527f387f44bfa622f5 zotero://select/groups/5435545/items/B5BL8UVM https://zotero.org/groups/5435545/items/B5BL8UVM https://linker.bio/cut:hash://md5/3c24d4cbaf3a45dea1f0debaf29daaf6!/b173052-175521 hash://md5/26f7ce5dd404e33c6570edd4ba250d20 https://dx.doi.org/10.5281/zenodo.1410543 https://dx.doi.org/10.5281/zenodo.13467175 Biodiversity Mammalia Chiroptera Chordata Animalia bats bat JournalArticle ScholarlyArticle article-journal 2015 ftdatacite https://doi.org/10.5281/zenodo.1346717410.5281/zenodo.141054310.5281/zenodo.13467175 2024-09-02T10:15:45Z (Uploaded by Plazi for the Bat Literature Project) The development of a rapid method for identification of the fifteen common species of British bat is described: Barbastella barbastellus, Eptesicus serotinus, Myotis bechsteinii, Myotis daubentonii, Myotis mystacinus, Myotis nattereri, Nyctalus leisleri, Nyctalus noctula, Plecotus auritus, Plecotus austriacus, Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Rhinolophus ferrumequinum and Rhinolophus hipposideros. A single multiplex PCR containing five fluorescently-tagged primers and fifteen species-specific primers targeting the mitochondrial control region and genes for cytb and ND1 produces clear peaks that are unique to each species when visualised on a DNA sequencer. The multiplex also includes primers to test for PCR-amplifiable bat DNA. This method will facilitate high throughput species identification of British bats, particularly in cases where phenotypic identification is difficult. ... Article in Journal/Newspaper Barbastella barbastellus Myotis nattereri Nyctalus noctula Pipistrellus nathusii Pipistrellus pipistrellus DataCite |
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unknown |
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Biodiversity Mammalia Chiroptera Chordata Animalia bats bat |
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Biodiversity Mammalia Chiroptera Chordata Animalia bats bat Hamilton, P. B. Uren Webster, T. M. Basiewicz, M. Kennedy, E. V. De-Bastos, E. S. R. Mathews, F. A rapid PCR-based test for identification of fifteen British bat species ... |
topic_facet |
Biodiversity Mammalia Chiroptera Chordata Animalia bats bat |
description |
(Uploaded by Plazi for the Bat Literature Project) The development of a rapid method for identification of the fifteen common species of British bat is described: Barbastella barbastellus, Eptesicus serotinus, Myotis bechsteinii, Myotis daubentonii, Myotis mystacinus, Myotis nattereri, Nyctalus leisleri, Nyctalus noctula, Plecotus auritus, Plecotus austriacus, Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Rhinolophus ferrumequinum and Rhinolophus hipposideros. A single multiplex PCR containing five fluorescently-tagged primers and fifteen species-specific primers targeting the mitochondrial control region and genes for cytb and ND1 produces clear peaks that are unique to each species when visualised on a DNA sequencer. The multiplex also includes primers to test for PCR-amplifiable bat DNA. This method will facilitate high throughput species identification of British bats, particularly in cases where phenotypic identification is difficult. ... |
format |
Article in Journal/Newspaper |
author |
Hamilton, P. B. Uren Webster, T. M. Basiewicz, M. Kennedy, E. V. De-Bastos, E. S. R. Mathews, F. |
author_facet |
Hamilton, P. B. Uren Webster, T. M. Basiewicz, M. Kennedy, E. V. De-Bastos, E. S. R. Mathews, F. |
author_sort |
Hamilton, P. B. |
title |
A rapid PCR-based test for identification of fifteen British bat species ... |
title_short |
A rapid PCR-based test for identification of fifteen British bat species ... |
title_full |
A rapid PCR-based test for identification of fifteen British bat species ... |
title_fullStr |
A rapid PCR-based test for identification of fifteen British bat species ... |
title_full_unstemmed |
A rapid PCR-based test for identification of fifteen British bat species ... |
title_sort |
rapid pcr-based test for identification of fifteen british bat species ... |
publisher |
Zenodo |
publishDate |
2015 |
url |
https://dx.doi.org/10.5281/zenodo.13467174 https://zenodo.org/doi/10.5281/zenodo.13467174 |
genre |
Barbastella barbastellus Myotis nattereri Nyctalus noctula Pipistrellus nathusii Pipistrellus pipistrellus |
genre_facet |
Barbastella barbastellus Myotis nattereri Nyctalus noctula Pipistrellus nathusii Pipistrellus pipistrellus |
op_relation |
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op_doi |
https://doi.org/10.5281/zenodo.1346717410.5281/zenodo.141054310.5281/zenodo.13467175 |
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1810433743426420736 |