Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ...

• Main Authors: Lelli, D., Moreno, A., Lavazza, A., Bresaola, M., Canelli, E., Boniotti, M. B., Cordioli, P.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Zenodo 2013
• Subjects: Biodiversity; Mammalia; Chiroptera; Chordata; Animalia; bats; bat; Vespertilio murinus
• Online Access: https://dx.doi.org/10.5281/zenodo.13445496; https://zenodo.org/doi/10.5281/zenodo.13445496

(Uploaded by Plazi for the Bat Literature Project) This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested. In total, 112 carcasses of bats found dead, and 44 faecal samples were analysed. Nineteen viral strains were isolated by in vitro cell culture from faecal and tissue samples of different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros and Vespertilio murinus), and they were morphologically identified as reoviruses by negative staining electron microscopy observation. The definitive assignment of all isolates to MRV was confirmed by RT-PCR assays targeting the L1 gene. Through a multiplex RTPCR assay targeting the S1 gene, we typed 15 of 19 isolates as MRV type 3. Partial L1 (416 bp) and complete S1 (1416 bp) sequences of the isolates were analysed and compared with those of reference ...