Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ...

• Main Author: Mudasar Hussain, Mohsin Masood, Laiba Nawaz, Naseem Akhtar, Hamad Alam, Mehwish Shaukat, Muhammad Shabaan, Mujeeb Ullah, Atif1, Waqas Ali
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Zenodo 2024
• Subjects: Rattus rattus
• Online Access: https://dx.doi.org/10.5281/zenodo.12706246; https://zenodo.org/doi/10.5281/zenodo.12706246

The present study, conducted from August to November 2022 in district Okara, Pakistan, focused on assessing the bacterial characterization in oral saliva swabs of black rats (Rattus rattus). DNA extraction was performed using the QIAamp DNA Microbiome kit, and the 16S rRNA gene was amplified using universal primers to amplify variable regions (V) V1 to V8 of 1380 bp. The identified bacterial phyla were as follows: Proteobacteria 98%, Firmicutes 1%, Actinobacteria 0.4%, and Bacteroidetes 0.05%. The bacterial classes included Gammaproteobacteria 95%, Alpha Enterobacterales 3%, and Bacilli 1%. The relative abundance of different bacterial orders was Pseudomonadales 40%, Enterobacterales 30%, Xanthomonadales 25%, Sphingomonadales 3%, Lactobacillales 1%, Micrococcales 0.4%, and Bacteroidales 0.05%. The identified families followed the order of Pseudomonadaceae 40%, Enterobacteriaceae 30%, Xanthomonadaceae 25%, and Sphingomonadaceae 3%. The percentage distribution of Pseudomonas was 40%, Stenotrophomonas 25%, ...