Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ...
The present study, conducted from August to November 2022 in district Okara, Pakistan, focused on assessing the bacterial characterization in oral saliva swabs of black rats (Rattus rattus). DNA extraction was performed using the QIAamp DNA Microbiome kit, and the 16S rRNA gene was amplified using u...
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ftdatacite:10.5281/zenodo.12706245 2024-09-15T18:31:53+00:00 Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ... Mudasar Hussain, Mohsin Masood, Laiba Nawaz, Naseem Akhtar, Hamad Alam, Mehwish Shaukat, Muhammad Shabaan, Mujeeb Ullah, Atif1, Waqas Ali 2024 https://dx.doi.org/10.5281/zenodo.12706245 https://zenodo.org/doi/10.5281/zenodo.12706245 unknown Zenodo https://dx.doi.org/10.5281/zenodo.12706246 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 JournalArticle ScholarlyArticle article-journal 2024 ftdatacite https://doi.org/10.5281/zenodo.1270624510.5281/zenodo.12706246 2024-08-01T10:09:00Z The present study, conducted from August to November 2022 in district Okara, Pakistan, focused on assessing the bacterial characterization in oral saliva swabs of black rats (Rattus rattus). DNA extraction was performed using the QIAamp DNA Microbiome kit, and the 16S rRNA gene was amplified using universal primers to amplify variable regions (V) V1 to V8 of 1380 bp. The identified bacterial phyla were as follows: Proteobacteria 98%, Firmicutes 1%, Actinobacteria 0.4%, and Bacteroidetes 0.05%. The bacterial classes included Gammaproteobacteria 95%, Alpha Enterobacterales 3%, and Bacilli 1%. The relative abundance of different bacterial orders was Pseudomonadales 40%, Enterobacterales 30%, Xanthomonadales 25%, Sphingomonadales 3%, Lactobacillales 1%, Micrococcales 0.4%, and Bacteroidales 0.05%. The identified families followed the order of Pseudomonadaceae 40%, Enterobacteriaceae 30%, Xanthomonadaceae 25%, and Sphingomonadaceae 3%. The percentage distribution of Pseudomonas was 40%, Stenotrophomonas 25%, ... Article in Journal/Newspaper Rattus rattus DataCite |
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The present study, conducted from August to November 2022 in district Okara, Pakistan, focused on assessing the bacterial characterization in oral saliva swabs of black rats (Rattus rattus). DNA extraction was performed using the QIAamp DNA Microbiome kit, and the 16S rRNA gene was amplified using universal primers to amplify variable regions (V) V1 to V8 of 1380 bp. The identified bacterial phyla were as follows: Proteobacteria 98%, Firmicutes 1%, Actinobacteria 0.4%, and Bacteroidetes 0.05%. The bacterial classes included Gammaproteobacteria 95%, Alpha Enterobacterales 3%, and Bacilli 1%. The relative abundance of different bacterial orders was Pseudomonadales 40%, Enterobacterales 30%, Xanthomonadales 25%, Sphingomonadales 3%, Lactobacillales 1%, Micrococcales 0.4%, and Bacteroidales 0.05%. The identified families followed the order of Pseudomonadaceae 40%, Enterobacteriaceae 30%, Xanthomonadaceae 25%, and Sphingomonadaceae 3%. The percentage distribution of Pseudomonas was 40%, Stenotrophomonas 25%, ... |
format |
Article in Journal/Newspaper |
author |
Mudasar Hussain, Mohsin Masood, Laiba Nawaz, Naseem Akhtar, Hamad Alam, Mehwish Shaukat, Muhammad Shabaan, Mujeeb Ullah, Atif1, Waqas Ali |
spellingShingle |
Mudasar Hussain, Mohsin Masood, Laiba Nawaz, Naseem Akhtar, Hamad Alam, Mehwish Shaukat, Muhammad Shabaan, Mujeeb Ullah, Atif1, Waqas Ali Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ... |
author_facet |
Mudasar Hussain, Mohsin Masood, Laiba Nawaz, Naseem Akhtar, Hamad Alam, Mehwish Shaukat, Muhammad Shabaan, Mujeeb Ullah, Atif1, Waqas Ali |
author_sort |
Mudasar Hussain, Mohsin Masood, Laiba Nawaz, Naseem Akhtar, Hamad Alam, Mehwish Shaukat, Muhammad Shabaan, Mujeeb Ullah, Atif1, Waqas Ali |
title |
Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ... |
title_short |
Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ... |
title_full |
Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ... |
title_fullStr |
Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ... |
title_full_unstemmed |
Characterization of oral microbiome from black rat (Rattus rattus) and assessment for pathogenicity ... |
title_sort |
characterization of oral microbiome from black rat (rattus rattus) and assessment for pathogenicity ... |
publisher |
Zenodo |
publishDate |
2024 |
url |
https://dx.doi.org/10.5281/zenodo.12706245 https://zenodo.org/doi/10.5281/zenodo.12706245 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.5281/zenodo.12706246 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.5281/zenodo.1270624510.5281/zenodo.12706246 |
_version_ |
1810473614047182848 |