Use Of Antibiotics To Optimize Micropropagation Of Betula Pendula Var. Carelica Merckl.

• Main Author: Kontsevaya, I.
• Format: Text
• Language: Russian
• Published: Zenodo 2018
• Subjects: regenerative capacity; culture in vitro; antibiotics; birch; karelian
• Online Access: https://dx.doi.org/10.5281/zenodo.1246064; https://zenodo.org/record/1246064

Dependence of the regenerative capacity of single-bud segments of Betula pendula Roth. var. carelica Merckl. shoots on the presence of some antibiotics in a nutrient medium are discussed in the paper. Methods: cell culture in vitro; statistical analysis. Β–lactam antibiotics are optimal at the stage of multiplication of Karelian birch in vitro with use of single–bud shoot segments as explants. Addition of carbenicillin (500 mg/l) and/or cefotaxime (500 mg/l) to the growth medium allows supporting visually clean tissue culture with no significant negative impact on morphometric parameters and viability of micro–plants. : Работа выполнена при поддержке ГПНИ (№ темы М16-33). : {"references": ["Koncevaya, I. I. (2008). Effect of cytokinins on morphogenesis in the culture of birch leaf explants. Forest Institute of the National Academy of Sciences of Belarus, (68), 205-213.", "Zhivulkina, E. V. (2005). Karelian birch in Belarus: resources, structure and condition of plantings. Botany: research, (33), 135-146.", "Dunaeva, S. Ye., & Osledkin, Yu. S. (2015). Bacterial microorganisms associated with plant tissues in culture in vitro: identification and possible role. Agricultural Biology, 5(1). 3-15", "Lloyd, G., & McCown, B. (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture., 30, 421-427.", "Koncevaya, I. I., & Zhadko, S. V. (2016). Effektivnost primeneniya antibiotikov na ehtape mul'tiplikacii pri klonirovanii breezy. Izvestiya Gomelskogo gosudarstvennogo universiteta imeni F. Skoriny, (6). 24-30.", "Dodds J. H., & Roberts L. W. (1982). Experiments in plant tissue culture. Aseptic techniques. Cambridge: Cambridge Univ. Press. U.K. 24."]}