Screening & Isolation Of Extracellular Thermo-Stable Enzymes Produced By Bacterial Isolates From Salbardi Hot Spring Region

• Main Authors: Dixit, Madhuri A., Ingle, Arun B., Charde, Vijay N., Swapnil P. Magar
• Format: Text
• Language: unknown
• Published: Zenodo 2018
• Subjects: Indian; Josephine; Psi; Randall; Verma; Kamchatka
• Online Access: https://dx.doi.org/10.5281/zenodo.1194912; https://zenodo.org/record/1194912

A wide range of thermo-stable enzymes is extracted from thermophilic bacteria from hot springs which are utilized in industries and in research. Proteases are the important range of thermo-stable enzymes which constitutes 59% of the global market share of industrial enzymes. The present study is based on Salbardi hot spring in central India which is known for its medically important hot spring water and its different chemical composition. This study is the first microbiological phylogenetic characterization of soil and water samples from salbardi hot spring region. The study reveals the presence of wide range of thermo-tolerant organisms specifically firmicutes, bacilli which are able to give optimum growth at a higher temperature range of 55-80°C., these bacteria are the major source of economically important thermo-stable enzymes. Protease production at 60°C was shown in this study also its stability from 50-90°C was checked at different incubation time representing maximum enzyme activity 178.95-216.84 U/ml. Salbardi hot spring is proved to be a wide source for thermo-stable bacteria as well as thermo-stable enzymes. : {"references": ["1.\tBrock T D, Freeze H. (1969). Thermus aquaticus gen. n., a non-sporulating extreme thermophile. Journal of Bacteriology. , 289-287. 2.\tKublanov I.V., Perevalova A., Slobodkina, G.B., Lebedinsky, A.V., Bidzhieva, S.K., Kolganova, T.V., et al. (2009). Biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of Uzon Caldera, Kamchatka (Russia). Appl. Environ. Microbiol 75. , 286-291. 3.\tSeatovic S., Glijik L., Radulovic Z and Jankov M.R.. (2004). Purification and partial characterization of superoxide dismutase from the thermophilic bacteria thermothrix sp. J. serb. Chem soc., 69(1) , 9-16. 4.\tDeng A, W.U J, Zhang Y., Zhang G. and Wen T. (2010). 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