A Piscan Ulcerative Aeromonas Infection

• Main Authors: Shnawa, Ibrahim M. S., Alsadi, Bashar A. H. E., Kalida K. Alniaem
• Format: Text
• Language: English
• Published: Zenodo 2015
• Subjects: Piscan; inflammatory respnonse; pure culture; pathogen; chronic; infection.; Beck; Indian; Morse; Stirling; Alaska; morse
• Online Access: https://dx.doi.org/10.5281/zenodo.1107212; https://zenodo.org/record/1107212

In the immunologic sense, clinical infection is a state of failure of the immune system to combat the pathogenic weapon of the bacteria invading the host. A motile gram negative vibroid organism associated with marked mono and poly nuclear cell responses was traced during the examination of a clinical material from an infected common carp Cyprinus carpio. On primary plate culture, growth was shown to be pure, dense population of an Aeromonas-like colony morphotype. The pure isolate was found to be; Aerobic, facultatively anaerobic, non-halophilic, grew at 0C, and 37C, oxidase positive utilizes glucose through fermentative pathway, resist 0/129 and novobiocin, produces alanine and lysine decarboxylases but non-producing ornithine dehydrolases. Tests for the in vitro determinants of pathogenicity has shown to be; Betahaemolytic onto blood agar, gelatinase, casienase and amylase producer. Three in vivo determinants of pathogenicity were tested as, the lethal dose fifty, the pathogenesis and pathogenicity. It was evident that 0.1 milliliter of the causal bacterial cell suspension of a density 1 x 107 CFU/ml injected intramuscularly into an average of 100gms fish toke five days incubation period, then at the day six morbidity and mortality were initiated. LD50 was recorded at the day 12 post-infection. Use of an LD50 doses to study the pathogenicity, reveals mononuclear and polynuclear cell responses, on examining the stained direct films of the clinical materials from the experimentally infected fish. Re-isolation tests confirm that the reisolant is same. The course of the infection in natural case was shown manifestation of; skin ulceration, haemorrhage and descaling. On evisceration, the internal organs were shown; congestion in the intestines, spleen and, air sacs. The induced infection showed a milder form of these manifestations. The grading of the virulence of this organism was virulent causing chronic course of infections as indicated from the pathogenesis and pathogenicity studies. Thus the infectious bacteria were consistent with Aeromonas hydrophila, and the infection was chronic. : {"references": ["Brooks GF, Caroll KC, Butel JS, Morse SA Mietzner TA, Jawetz,\nMelnik, and A Delbergs, Medical Microbiology, 26thed, McGraw-Hill,\nLanges 2013, W.-K.", "Levinson W, Review of Medical Microbiology and Immunology, 11th\ned. McGraw-Hill, Lange, 2010, New York.", "Ferichs GN, The isolation and Identification of fish bacterial Pathogens.\nInstitute of Aquaculture, University of Stirling, Scotland, 1984 UK.", "Roberts RJ, Fish Pathology, 4thed, Wiley Scientific, 2012, UK.", "Miyazaki T, Kageyama T, Miura M, Yoshida T, Histopathology of\nViremia-Associated ana-aki-byo in combination with Aeromonas\nhydrophila in color carp Cyprinus carpio in Japan, Dis. Aquatic Org.\n2001, 44:109-120.", "Abowei JFN, Briyai JF, A review of some bacterial diseases in African\nculture fisheries, Asia.J.Med.Sci.2011, 3(5):206-217.", "Carraschi SP, Cruz C, Neto JGM, Mores FR, Junior ODR, Neto AN,\nBotoluzzi NL. Evaluation of experimental infection with Aeromonas\nhydrophila in pacu (Piaractumes opotamicus Holemberg 1887), Int J\nFisheries and Aqua. 2012, 4(5):81-84.", "Cipriano RC, Aeromonas hydrophila and motile Aeromonas septicemia\nof fish, Fish and Wildlife Science Division of Fishery Research, 2001,\nWashington DC2040.", "Li C, Beck B, Su B, Terhune J, Peatman E, Early mucosal responses in\nblue catfish skin to Aeromonas hydropila infection, Fish and Shellfish\nImmunol, 2013,54;920-928\n[10] Meyers T R, Fish Pathology Section Laboratory Manual,Alaska\ndepartment of fish and commercial fisheries division,Special publication\nNo.12 2ndedP1 95,2000..\n[11] Das A, Vinayasree V, Santhoch CR, Hari SS, Aeromonas sobria,\nAeromonas hydrophila from commercial food stuffs and environmental\nsources. J Exp.Sci. 2012,3(9):36-42.\n[12] Olsen R, Ringo E, Svihus B, Criteria for safe use of plant ingredients in\ndiet for aquaculture and fish. Opinion of panl on Animal feed of\nNerwigian Scientific committee for food safety,2009,VKM.\n[13] Reed L J, Munch H, A simple method of estimating fifty percent\nendpoint, Am.J.Hygein,1938, 21(3):493-497.\n[14] Decostane A,H, Haesebrouk F, Charlier G, Ducatella R, The association\nof Flavobacterium colamar strains of high virulence and low virulence\nof gill tissue of black mollies P. sphenops, Vet.Microbiol.,1999.,4:287-\n298.\n[15] Zheng W, Cato K, Yang X, Grass carp (Ctenopharyn godonidenus),\ninfected with multiple strains of A hydrophila, Afr.Microbiol.Res,\n2012.6(31):4512-4520.\n[16] Sarkara MJA, Rashid MM, Pathogenicity of bacterial isolate of catfish,\ncarp and perch, J.Banladsh.Agr.Uni,2012,2,.10(1):157-161.\n[17] Tarasu T, Dhas KA, Velmurugan S,Viji VT, Kumaran T, Babu M,\nSelvaraj T, Isolation of Aeromonas hydrophila from infected ornemantal\nfish hatchery during massive disease outbreak, Int.J.Cur.Res.,\n2011,2(1):37-410.\n[18] Hussian MF, Rashid MM, Sayed MA, Experimental infection of\nindigenus climhy perch A. testudineus with Aeromonas hydrophila\nbacteria, Prog.Agri,2011,22(1&2):105-114.\n[19] Andaman, The fish disease due Aeromonas hydrophila, Central Indian\nInstitute, India, www.iisceractin/CurrSci./May10,2015articleNo.13,htm.\n[20] Ibrahim M, Mostfa MM, Arab RMH, Rezk MA, Prevalence of\nAeromonas hydrophila infection in wild and cultured fish Tilapia niotica\n(O. nilticus), Eygp.8th, 2008, Inter.Sympo. on Tilapia in aquaculture.\n[21] Rey A, Verjan N, Ferquson HW, Iregui C, Pathogenic Aeromonas\nhydrophila strain KJ99 infection and its extracellular products in two\nspecies of fish,Vet.Rec.,2009,164:493-499.\n[22] Morohoshi T, Inaba T, Kato N, Kanai K, Ikeda T, Identification of\nquorum-sensing signal molecules and Lux R1 homologs in fish pathogen\nEdwadsiella trada J.Biosci.Bioengin.,2004,98(4):284-291."]}