Histochemistry Of Intestinal Enzymes Of Juvenile Dourado Salminus Brasiliensis Fed Bovine Colostrum
Enzyme activity was evaluated in the intestine of juvenile dourado (Salminus brasiliensis) fed with diets containing 0, 10 or 20% of lyophilized bovine colostrum (LBC) inclusion for either 30 or 60 days. The intestinal enzymes acid and alkaline phosphatase (ACP and ALP, respectively), non-specific e...
Main Authors: | , , , , |
---|---|
Format: | Text |
Language: | English |
Published: |
Zenodo
2014
|
Subjects: | |
Online Access: | https://dx.doi.org/10.5281/zenodo.1096252 https://zenodo.org/record/1096252 |
Summary: | Enzyme activity was evaluated in the intestine of juvenile dourado (Salminus brasiliensis) fed with diets containing 0, 10 or 20% of lyophilized bovine colostrum (LBC) inclusion for either 30 or 60 days. The intestinal enzymes acid and alkaline phosphatase (ACP and ALP, respectively), non-specific esterase (NSE), lipase (LIP), dipeptidyl aminopeptidase IV (DAP IV) and leucine aminopeptidase (LAP) were studied using histochemistry in four intestinal segments (S1, S2, S3 and posterior intestine). Weak proteolitic activity was observed in all intestinal segments for DAP IV and LAP. The activity of NSE and LIP was also weak in all intestines, except for the moderate activity of NSE in the S2 of 20% LBC group after 30 days and in the S1 of 0% LBC group after 60 days. The ACP was detected only in the S2 and S3 of the 10% LBC group after 30 days. Moderate and strong staining was observed in the first three intestinal segments for ALP and weak activity in the posterior intestine. The activity of DAP IV, LAP and ALP were also present in the cytoplasm of the enterocytes. In the present results, bovine colostrum feeding did not cause alterations in activity of intestinal enzymes. : {"references": ["A. Huguet, B. S\u00e8ve, J. Le Dividich, and I. Le Hu\u00ebrou-Luron, \"Effects of\na bovine colostrum-supplemented diet on some gut parameters in\nweaned piglets\", Reproduction Nutrition Development, vol. 46, pp. 167-\n178, 2006.", "A.L. Lima, P. Pauletti, I. Susin, and R. Machado-Neto, \"Fluctuation of\nserum variables in goats and comparative study of antibody absorption\nin newborn kids using cattle and goat colostrum\", Brazilian Journal of\nAnimal Science, vol. 38, pp. 2211-2217, 2009.", "D.B. Moretti, L. Kindlein, P. Pauletti, and R. Machado-Neto, \"IgG\nabsorption by Santa Ines lambs fed Holstein bovine colostrum or Santa\nInes ovine colostrum\", Animal, vol. 4, pp. 933-937, 2010.", "A.P.O Rodrigues, P. Pauletti, L. Kindlein, J.E.P. Cyrino, E.F. Delgado,\nand R. Machado-Neto, \"Intestinal morphology and histology of the\nstriped catfish Pseudoplatystoma fasciatum (Linnaeus, 1766) fed dry\ndiets\", Aquaculture Nutrition, vol. 15, pp. 55-563, 2009.", "P. Bodammer, C. Maletzki, G. Waitz, and J. Emmrich, \"Prophylatic\napplication of bovine colostrum ameliorates murine colitis via induction\nof immunoregulatory cells\", Journal of Nutrition, vol. 141, pp. 1056-\n1061, 2011.", "D.B. Moretti, W.M. Nordi, A.L. Lima, P. Pauletti, I. Susin, and R.\nMachado-Neto, \"Enzyme activity in the small intestine of goat kids\nduring the period of passive immunity acquisition\", Small Ruminant\nResearch, vol. 105, pp. 321-328, 2012.", "N.N. Pandey, A.A. Dar, D.B. Mondal, and L. Nagaraja, \"Bovine\ncolostrum: A veterinary nutraceutical\", Journal of Veterinary Medicine\nand Animal Health, vol. 3, pp. 31-35, 2011.", "L.J. Schep, I.G. Tucker, G. Young, R. Ledger, and A.G. Butt,\n\"Controlled release opportunities for oral peptide delivery in\naquaculture\", Journal of Controlled Release, vol. 59, pp. 1-14, 1999.", "R.L. Barbieri, and F.J. Hern\u00e1ndez-Blazquez, \"An\u00e1lise ultra-estrutural da\nabsor\u00e7\u00e3o intestinal de macromol\u00e9cula prot\u00e9ica com o uso de peixe como\nmodelo experimental\", ConScientiae Sa\u00fade, vol. 1, pp. 21-30, 2002.\n[10] K. Opuszynski, and J.V. Shireman, \"Digestive mechanisms (book\nstyle)\". In Herbivorous Fishes: Culture and Use for Weed Management,\nK. Opuszynski and J.V. Shireman, Boca Raton, FL: CRC Press, 1995,\npp.21-31.\n[11] R. Ledger, I.G. Tucker, and G.F. Walker, \"The metabolic barrier of the\nlower intestinal tract of salmon to the oral delivery of protein and\npeptide drugs\", Journal of Controlled Release, vol. 85, pp. 91-103, 2002.\n[12] D.M. Fracalossi, G. Meyer, F.M. Santamaria, M. Weingastner, and E.\nZaniboni Filho, \"Desempenho do jundi\u00e1, Rhamdia quelen, e do dourado,\nSalminus brasiliensis, em viveiros de terra na Regi\u00e3o Sul do Brasil\",\nActa Scientiarum, vol. 26, pp. 345-352, 2004.\n[13] Association of Official Analytical Chemists. Official methods of\nanalysis, 18th ed. vol. 2, Arlington, VA: AOAC, 2004.\n[14] S.S. Rodrigues, and E. Menin, \"Anatomia do tubo digestivo de Salminus\nbrasiliensis (Cuvier, 1818) (Pisces, Characidae, Salmininae)\", Biotemas,\nvol. 21, pp. 65-75, 2008.\n[15] Z. Lodja, \"Studies on Dipeptidyl(Amino)Peptidase IV (Glycyl-Proline\nNaphthylamidase)\", Histochemistry, vol. 59, pp. 153-166, 1979.\n[16] J.D. Bancroft, \"Enzyme histochemistry\", in Theory and Practice of\nHistological Techniques, vol. 4, J.D. Bancroft, and A. Stevens), New\nYork, NY: Churchill Livingstone, 1996, pp.391-410.\n[17] K.N. Hirji, and W.A.M. Courtney \"Leucine aminopeptidase activity in\nthe digestive tract of perch, Perca fluviatilis L\", Journal of Fish Biology,\nvol. 21, pp. 615\u2013622, 1982.\n[18] A. Gawlicka, S. Teh, S.S.O. Hung, E. Hinton, and J. De Ll No\u00fce,\n\"Histological and histochemical changes in the digestive tract of white\nsturgeon larvae during ontogeny\", Fish Physiology and Biochemistry,\nvol. 14, pp. 357-371, 1995.\n[19] B. Tengjaroenkul, B.J. Smith, T. Caceci, and S.A. Smith, \"Distribution\nof intestinal enzyme activities along the intestinal tract of cultured Nile\ntilapia, Oreochromis niloticus L\", Aquaculture, vol. 182, pp. 317\u2013327,\n2000.\n[20] V.V. Kuz'mina, \"Classical and modern concepts in fish digestion\", in\nFeeding and Digestive Functions of Fishes, J.E.P Cyrino, D.B Bureau,\nand B.G. Kapoor, Enfield, NH: Science Publishers, 2008, pp. 85-154.\n[21] V.V. Kuz'mina, and A.G. Gelman, \"Membrane-linked digestion in fish\",\nReviews in Fisheries Science, vol. 5, pp. 99-129, 1997.\n[22] E. McLean, B. R\u00f8nsholdt, C. Sten, and Najamuddin, \"Gastrointestinal\ndelivery of peptide and protein drugs to aquaculture teleosts\",\nAquaculture, vol. 177, pp. 231-247, 1999.\n[23] J. Walford, and T.J. Lam, \"Development of digestive tract and\nproteolytic enzyme activity in seabass (Lates calcarifer) larvae and\njuveniles\", Aquaculture, vol. 109, pp. 187-05, 1993.\n[24] J.L. Zambonino Infante, and C.J. Cahu, \"High dietary lipid levels\nenhance digestive tract maturation and improve Dicentrarchus labrax\nlarval development\", Journal of Nutrition, vol. 129, pp. 1195-1200,\n1999.\n[25] V. Buchet, J.L. Zambonino Infante, and C.L. Cahu, \"Effect of lipid level\nin a compound diet on the development of red drum (Sciaenops\nocellatus) larvae\", Aquaculture, vol. 184, pp. 339-347, 2000.\n[26] M.S. Izquierdo, J. Socorro, L. Arantzamendi, and C.M. Hernandez-Cruz,\n\"Recent advances in lipid nutrition in fish larvae\", Fish Physiology and\nBiochemistry, vol. 22, pp. 97-107, 2000.\n[27] S. Morais, M. Lacuisse, L.E.C. Concei\u00e7\u00e3o, M.T. Dinis, and I.\nR\u00f8nnestad, \"Ontogeny of the digestive capacity of Senegalese sole\n(Solea senegalensis), with respect to digestion, absorption and\nmetabolism of amino acids from Artemia\", Marine Biology, vol. 145,\npp. 243-50, 2004.\n[28] B. Savona, C. Tramati, and A. Mazzola, \"Digestive enzymes in larvae\nand juveniles of farmed sharpsnout seabream (Diplodus puntazzo)\n(Cetti, 1777)\", The Open Marine Biology Journal, vol. 5, pp. 47-57,\n2011.\n[29] Z. Kozari\u0107, Z. Petrinec, S. Ku\u017eir, E. Gjur\u010devi\u0107, and B. Ba\u017edari\u0107,\n\"Histochemical analyses of digestive enzymes in the intestine of adult\nlarge-scale gurnard (Lepidotrigla cavillone, Lacep\u00e8de, 1801)\",\nAnatomia, Histologia, Embryologia, vol. 40, pp. 314-320, 2011.\n[30] K. Baintner, 1994, \"Demonstration of acidity intestinal vacuoles of the\nsuckling rat and pig\", Journal of Histochemistry and Cytochemistry, vol.\n42, pp. 231-238, 1994.\n[31] I. Hussain, and A. Channa, \"Histochemical distribution of lipase and\nacid phosphatase in the intestinal tract of the snow trout, Schizothorax\ncurvifrons Heckel\", Journal of Biological Sciences, vol. 10, pp. 643-647,\n2010.\n[32] Y. Dupuis, S. Tardivel, Z. Porembska, and P. Fournier, \"Effect of some\nphosphatase inhibitors on intestinal calcium transfer\", International\nJournal of Biochemistry, vol. 23, pp. 175\u2013180, 1991.\n[33] A. Mahmood, F. Yamagishi, R. Eliakim, K. DeSchryver-Kecskemeti,\nT.L. Gramlich, and D.H. Alpers, \"A possible role for rat intestinal\nsurfactant-like particles in transepithelial triacylglycerol transport\",\nJournal of Clinical Investigation, vol. 93, pp. 70\u201380, 1994.\n[34] Z. Kozari\u0107, S. Ku\u017eir, Z. Petrinec, E. Gjur\u010devi\u0107, and A. Opa\u010dak,\n\"Histochemical distribution of digestive enzymes in intestine of\ngoldline, Sarpa salpa L. 1758\", Journal of Applied Ichthyology, vol. 22,\npp. 43-48., 2006."]} |
---|