Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis

Fishes, in general, follow ammonotelic mode of excretion. However, certain stress factors may provoke them to excrete urea. In the present study, the possible role of ureogenesis to avoid accumulation of toxic ammonia under water-restricted condition was tested in Heteropneustes fossilis . A total o...

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Main Authors: Shuvasish Roy Choudhury, Mahanta, Rita, Borkotoki, Aparajita
Format: Text
Language:English
Published: Zenodo 2014
Subjects:
Online Access:https://dx.doi.org/10.5281/zenodo.1091086
https://zenodo.org/record/1091086
id ftdatacite:10.5281/zenodo.1091086
record_format openpolar
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language English
topic Ammonia
aquarium
glutamate dehydrogenase
urea
ureogenesis.
spellingShingle Ammonia
aquarium
glutamate dehydrogenase
urea
ureogenesis.
Shuvasish Roy Choudhury
Mahanta, Rita
Borkotoki, Aparajita
Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis
topic_facet Ammonia
aquarium
glutamate dehydrogenase
urea
ureogenesis.
description Fishes, in general, follow ammonotelic mode of excretion. However, certain stress factors may provoke them to excrete urea. In the present study, the possible role of ureogenesis to avoid accumulation of toxic ammonia under water-restricted condition was tested in Heteropneustes fossilis . A total of hundred fishes were collected and sacrificed. Excretory urea and ammonia were estimated in the water of the aquarium and glutamate dehydrogenase acitivity was measured in the hepatic tissue. During the experimental period, excretory ammonia in Heteropneustes fossilis was found between 931% to 16% above the baseline ammonia and excretory urea was found between 112% to 898% above the baseline urea. A high degree of correlation with r (coefficient of correlation) above 0.9 is observed between excretory ammonia and urea in Heteropneustes fossilis. However, only a moderate degree of correlation is observed between the activity of glutamate dehydrogenase and excretory ammonia and urea. : {"references": ["Anderson, P. M., Urea and Glutamine Synthesis: Environmental Influences on Nitrogen Excretion. Fish Physiology, 20: 239-277, 2001.", "Chadwick, T., and Wright,P., Nitrogen Excretion and Expression of Urea Cycle Enzymes in the Atlantic Cod (Gadus morhua): A Comparison of Early Life Stages with Adults. Journal of Experimental Biology, 202: 2653-2662, 1999.", "Chew, S. F., Ong,T. F., Ho, L., Tam, W. L., Loong, A. M., Hiong, K. C.,Wong,W.P., \tand Ip,Y.K., Urea Synthesis in the African Lungfish Protopterus dolloi \u2013 Hepatic Carbamoyl Phosphate Synthetase III and Glutamine Synthetase Can Be Upregulated by Six Days of Aerial Exposure. Journal of Experimental Biology, 206: 3615-3624, 2003.", "Cammaerts, D., and Jacobs, M., A Study of the Role of Glutamate Dehydrogenase in the Nitrogen Metabolism of Arabidopsis thaliana. Planta, 163 (4): 517-526, 1984.", "Choudhury. R. S. and Mahanta. R (2013): \"Status of Blood Ammonia and Urea with Reference to Hepatic Glutamate Dehydrogenase Activity in Freshwater Airbreathing Teleost, Heteropneustes fossilis Kept in a Water-Restricted Condition\" IJSER, 4 (5), pp. 1919-1323.", "Hirata, T., Kaneko, T., Ono, T., Nakazato, T., Furukawa, N., Sanae, H., Shigeo, W., Munekazu, S., Min-Hwang, C., Michael, R.F., and Shigehisa, H., Mechanism of Acid Adaptation of a Fish Living in a pH 3.5 lake. The American Physiological Society. APS Manuscript No. \u2013 R, 267-2, 2003.", "Ip, Y. K., Chew, S. F., Leong, I. W. A., Jin, Y., and Wu, R. S. S., The Sleeper Bostrichthyes sinensis (Teleost) Stores Glutamine and Reduces Ammonia Production during Aerial Exposure. Journal of Comparative Physiology, 171: 357-367, 2001.", "Ip, Y. K., Lim, C. K., Lee, S. L. M., Wong, W. P., and Chew, S. F., Postprandial Increases in Nitrogenous Excretion and Urea Synthesis in the Giant Mudskipper Periophthalmodon schlosseri. Journal of Experimental Biology, 207: 3015-3023, 2004.", "Janssens, P. A., The Metabolism of Aestivating African Lungfish. Comparative Biochemistry and Physiology, 11: 105-117, 1964.\n[10]\tLim, C. B., Anderson, P. M., Chew, S. F., and Ip, Y. K., Reduction in the Rates of Protein and Amino Acid Catabolism to Slow Down the Accumulation of Endogenous Ammonia: A Strategy Potentially Adopted by Mudskippers (Periophthalmodon schlosseri and Boleophthalmus boddaerti) during Aerial Exposure in Constant Darkness. Journal of Experimental Biology, 204: 1605-1614, 2001.\n[11]\tMommsen, T.P., and Walsh, P. J., Biochemical and Environmental Perspectives on Nitrogen Metabolism in Fishes. Cellular and Molecular Life Sciences, 48 (6): 583-593, 2005.\n[12]\tSaha, N. and Ratha, B. K., Active Ureogenesis in a Freshwater Air-Breathing teleost, Heteropneustes fossilis. Journal of Experimental Zoology, 241: 137-141, 1987.\n[13]\tSaha, N. and Ratha, B. K., Comparative Study of Ureogenesis in Freshwater Air-Breathing Teleosts. Journal of Experimental Zoology, 252: 1-8, 1989.\n[14]\tSaha, N. and Ratha, B. K., Alterations in the Excretion Pattern of Ammonia and Urea in a Freshwater Air-Breathing Teleost, Heteropneustes fossilis (Bloch) during Hyper-Ammonia Stress. Indian Jounal of Experimental Biology, 28: 597-599, 1990.\n[15]\tSaha, N. and Ratha, B. K., Induction of Ornithine-Urea Cycle in a Freshwater Teleost, Heteropneustes fossilis, Exposed to High Concentrations of Ammonium Chloride. Comparative Biochemistry and Physiology, 108: 315-325, 1994.\n[16]\tSaha, N., Datta, S., Biswas, K., and Kharbuli, Z. Y., Role of Ureogenesis in Tackling Problems of Ammonia Toxicity during Exposure to Higher Ambient Ammonia in the Air-Breathing Walking Catfish Clarias batrachus. Journal of Bioscience, 28(6): 733-742, 2003.\n[17]\tCampbell, J. W., and Anderson, P. M., Evolution of mitochondrial enzyme system in fish: the mitochondrial synthesis of glutamine and citrulline. In: Molecular biology of fishes (eds: P.W.Hochachka and T.P.Mommsen) (Elsevier: Amsterdam) 1: 43-76, 1991.\n[18]\tAnken H.C., Schiphorst M.E., Kinetic determination of ammonia in plasma. Clin. Chim. Acta. 1974;56:151\u2013157. doi: 10.1016/0009-8981(74)90223-X. 56(2):151\u2013157, 1974.\n[19]\tFawcett JK, Scott JE., A rapid and precise method for the determination of urea. J Clin Patho,; 13: 156-159, 1960.\n[20]\tDohertry, D., In Methods of Enzymol., 17, Part A. (eds. Tabor, H., and Tabor, C. W.) 850, 1970."]}
format Text
author Shuvasish Roy Choudhury
Mahanta, Rita
Borkotoki, Aparajita
author_facet Shuvasish Roy Choudhury
Mahanta, Rita
Borkotoki, Aparajita
author_sort Shuvasish Roy Choudhury
title Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis
title_short Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis
title_full Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis
title_fullStr Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis
title_full_unstemmed Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis
title_sort glutamate dehydrogenase and the changing pattern of excretory ammonia and urea in heteropneustes fossilis
publisher Zenodo
publishDate 2014
url https://dx.doi.org/10.5281/zenodo.1091086
https://zenodo.org/record/1091086
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geographic Chadwick
Indian
SANAE
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geographic_facet Chadwick
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SANAE
Tabor
genre atlantic cod
Gadus morhua
genre_facet atlantic cod
Gadus morhua
op_relation https://dx.doi.org/10.5281/zenodo.1091085
op_rights Open Access
Creative Commons Attribution 4.0
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
op_rightsnorm CC-BY
op_doi https://doi.org/10.5281/zenodo.1091086
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spelling ftdatacite:10.5281/zenodo.1091086 2023-05-15T15:27:53+02:00 Glutamate Dehydrogenase And The Changing Pattern Of Excretory Ammonia And Urea In Heteropneustes Fossilis Shuvasish Roy Choudhury Mahanta, Rita Borkotoki, Aparajita 2014 https://dx.doi.org/10.5281/zenodo.1091086 https://zenodo.org/record/1091086 en eng Zenodo https://dx.doi.org/10.5281/zenodo.1091085 Open Access Creative Commons Attribution 4.0 https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess CC-BY Ammonia aquarium glutamate dehydrogenase urea ureogenesis. Text Journal article article-journal ScholarlyArticle 2014 ftdatacite https://doi.org/10.5281/zenodo.1091086 https://doi.org/10.5281/zenodo.1091085 2021-11-05T12:55:41Z Fishes, in general, follow ammonotelic mode of excretion. However, certain stress factors may provoke them to excrete urea. In the present study, the possible role of ureogenesis to avoid accumulation of toxic ammonia under water-restricted condition was tested in Heteropneustes fossilis . A total of hundred fishes were collected and sacrificed. Excretory urea and ammonia were estimated in the water of the aquarium and glutamate dehydrogenase acitivity was measured in the hepatic tissue. During the experimental period, excretory ammonia in Heteropneustes fossilis was found between 931% to 16% above the baseline ammonia and excretory urea was found between 112% to 898% above the baseline urea. A high degree of correlation with r (coefficient of correlation) above 0.9 is observed between excretory ammonia and urea in Heteropneustes fossilis. However, only a moderate degree of correlation is observed between the activity of glutamate dehydrogenase and excretory ammonia and urea. : {"references": ["Anderson, P. M., Urea and Glutamine Synthesis: Environmental Influences on Nitrogen Excretion. Fish Physiology, 20: 239-277, 2001.", "Chadwick, T., and Wright,P., Nitrogen Excretion and Expression of Urea Cycle Enzymes in the Atlantic Cod (Gadus morhua): A Comparison of Early Life Stages with Adults. Journal of Experimental Biology, 202: 2653-2662, 1999.", "Chew, S. F., Ong,T. F., Ho, L., Tam, W. L., Loong, A. M., Hiong, K. C.,Wong,W.P., \tand Ip,Y.K., Urea Synthesis in the African Lungfish Protopterus dolloi \u2013 Hepatic Carbamoyl Phosphate Synthetase III and Glutamine Synthetase Can Be Upregulated by Six Days of Aerial Exposure. Journal of Experimental Biology, 206: 3615-3624, 2003.", "Cammaerts, D., and Jacobs, M., A Study of the Role of Glutamate Dehydrogenase in the Nitrogen Metabolism of Arabidopsis thaliana. Planta, 163 (4): 517-526, 1984.", "Choudhury. R. S. and Mahanta. R (2013): \"Status of Blood Ammonia and Urea with Reference to Hepatic Glutamate Dehydrogenase Activity in Freshwater Airbreathing Teleost, Heteropneustes fossilis Kept in a Water-Restricted Condition\" IJSER, 4 (5), pp. 1919-1323.", "Hirata, T., Kaneko, T., Ono, T., Nakazato, T., Furukawa, N., Sanae, H., Shigeo, W., Munekazu, S., Min-Hwang, C., Michael, R.F., and Shigehisa, H., Mechanism of Acid Adaptation of a Fish Living in a pH 3.5 lake. The American Physiological Society. APS Manuscript No. \u2013 R, 267-2, 2003.", "Ip, Y. K., Chew, S. F., Leong, I. W. A., Jin, Y., and Wu, R. S. S., The Sleeper Bostrichthyes sinensis (Teleost) Stores Glutamine and Reduces Ammonia Production during Aerial Exposure. Journal of Comparative Physiology, 171: 357-367, 2001.", "Ip, Y. K., Lim, C. K., Lee, S. L. M., Wong, W. P., and Chew, S. F., Postprandial Increases in Nitrogenous Excretion and Urea Synthesis in the Giant Mudskipper Periophthalmodon schlosseri. Journal of Experimental Biology, 207: 3015-3023, 2004.", "Janssens, P. A., The Metabolism of Aestivating African Lungfish. Comparative Biochemistry and Physiology, 11: 105-117, 1964.\n[10]\tLim, C. B., Anderson, P. M., Chew, S. F., and Ip, Y. K., Reduction in the Rates of Protein and Amino Acid Catabolism to Slow Down the Accumulation of Endogenous Ammonia: A Strategy Potentially Adopted by Mudskippers (Periophthalmodon schlosseri and Boleophthalmus boddaerti) during Aerial Exposure in Constant Darkness. Journal of Experimental Biology, 204: 1605-1614, 2001.\n[11]\tMommsen, T.P., and Walsh, P. J., Biochemical and Environmental Perspectives on Nitrogen Metabolism in Fishes. Cellular and Molecular Life Sciences, 48 (6): 583-593, 2005.\n[12]\tSaha, N. and Ratha, B. K., Active Ureogenesis in a Freshwater Air-Breathing teleost, Heteropneustes fossilis. Journal of Experimental Zoology, 241: 137-141, 1987.\n[13]\tSaha, N. and Ratha, B. K., Comparative Study of Ureogenesis in Freshwater Air-Breathing Teleosts. Journal of Experimental Zoology, 252: 1-8, 1989.\n[14]\tSaha, N. and Ratha, B. K., Alterations in the Excretion Pattern of Ammonia and Urea in a Freshwater Air-Breathing Teleost, Heteropneustes fossilis (Bloch) during Hyper-Ammonia Stress. Indian Jounal of Experimental Biology, 28: 597-599, 1990.\n[15]\tSaha, N. and Ratha, B. K., Induction of Ornithine-Urea Cycle in a Freshwater Teleost, Heteropneustes fossilis, Exposed to High Concentrations of Ammonium Chloride. Comparative Biochemistry and Physiology, 108: 315-325, 1994.\n[16]\tSaha, N., Datta, S., Biswas, K., and Kharbuli, Z. Y., Role of Ureogenesis in Tackling Problems of Ammonia Toxicity during Exposure to Higher Ambient Ammonia in the Air-Breathing Walking Catfish Clarias batrachus. Journal of Bioscience, 28(6): 733-742, 2003.\n[17]\tCampbell, J. W., and Anderson, P. M., Evolution of mitochondrial enzyme system in fish: the mitochondrial synthesis of glutamine and citrulline. In: Molecular biology of fishes (eds: P.W.Hochachka and T.P.Mommsen) (Elsevier: Amsterdam) 1: 43-76, 1991.\n[18]\tAnken H.C., Schiphorst M.E., Kinetic determination of ammonia in plasma. Clin. Chim. Acta. 1974;56:151\u2013157. doi: 10.1016/0009-8981(74)90223-X. 56(2):151\u2013157, 1974.\n[19]\tFawcett JK, Scott JE., A rapid and precise method for the determination of urea. J Clin Patho,; 13: 156-159, 1960.\n[20]\tDohertry, D., In Methods of Enzymol., 17, Part A. (eds. Tabor, H., and Tabor, C. W.) 850, 1970."]} Text atlantic cod Gadus morhua DataCite Metadata Store (German National Library of Science and Technology) Chadwick ENVELOPE(160.433,160.433,-72.500,-72.500) Indian SANAE ENVELOPE(-2.850,-2.850,-71.667,-71.667) Tabor ENVELOPE(150.284,150.284,71.277,71.277)