Quantitative Characteristics Of Rainbow Trout, Oncorhynchus Mykiss, Neo-Males (Xx Genotype) And Super-Males (Yy Genotype) Sperm

• Main Authors: Radosław K. Kowalski, Sarosiek, Beata, Wiesław Demianowicz, Jędrek Judek, Goryczko, Krzysztof, Dobosz, Stefan, Kuźmiński, Henryk, Demska-Zakęś, Krystyna, Babiak, Igor, Glogowski, Jan
• Format: Text
• Language: English
• Published: Zenodo 2011
• Subjects: fish; genotype; rainbow trout; sperm.; Donaldson; Gjerde; McAllister; Searle; Sockeye; Atlantic salmon; Turbot
• Online Access: https://dx.doi.org/10.5281/zenodo.1071583; https://zenodo.org/record/1071583

Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in samples of stripped and testicular sperm obtained from XY and YY males, and testicular sperm of XX males. The motile spermatozoa, as measured by both subjective method and CASA, showed no differences between testicular sperm of XX males and stripped sperm of XY and YY males whereas testicular sperm of XY and YY males had significantly lower sperm motility. Result of protein densitometry showed similarities in protein profile between seminal plasma of XY and YY males and testicular fluids of XX males. Testis of XX males showed specific histological structures of cysts consists hypertrophied Sertoli cells. : {"references": ["G. H. Aas, T. Refstic and B. Gjerde, \"Evaluation of milt quality od Atlantic salmon\". Aquaculture 95: 125-132, 1991.", "I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, P. Brzuzan and S.\nCiesielski, \"Androgenesis in rainbow trout using cryopreserved\nspermatozoa: the effect of processing and biological factors\". Theriogenology 57(4): 1229-1249, 2002b.", "I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, K. Goryczko, S.\nCiesielski, P. Brzuzan, B. Urbanyi, A. Horvarth, F. Lahnsteiner and J.\nPiironen, \"Failure of interspecies androgenesis in salmonids\". J. Fish\nBiol. 61: 432-447, 2002a.", "K. Bieniarz, K. Goryczko, S. Dobosz and T. Grudniewski, \"The effects\nof 17a-methylotestosterone on rainbow trout (Oncorhynchus mykiss)\".\nPol. Arch. Hydrobiol. 38: 295-301, 1991.", "R. Billard, \"Utilisation d-un systeme tri-glycocolle pour tamponer le\ndilueur d-insemination pour truite\" Bulletin Fran\u00e7ais de P\u00eache et\nPisciculture 264: 102-112, 1977.", "R. Billard, J. Cosson, G. Perchec and O. Linhart, \"Biology of sperm and\nartificial reproduction in carp\". Aquaculture 129: 95-112, 1995.", "V. J. Bye and R. F. Lincoln, \"Commercial methods for the control of\nsexual maturation in rainbow trout (Salmo gairdneri) \". Aquaculture 57:\n299-310, 1986.", "D. Chourrout \"Pressure-induced retention of second polar body and\nsuppression of first cleavage in rainbow trout: production of all-triploids,\nall-tetraploids, and heterozygous and homozygous diploid\ngynogenetics\". Aquaculture 36: 111-26, 1984.", "A. Ciereszko and K. Dabrowski, \"Estimation of sperm concentration of\nrainbow trout, whitefish and yellow perch using spectrophotometric\ntechnique\". Aquaculture 109: 367-373, 1993.\n[10] A. Ciereszko and K. Dabrowski, \"Relationship between biochemical\nconstituents of fish sperm and fertility: the effect of short-term storage\".\nFish Physiol Biochem. 12: 357-367, 1994\n[11] A. Ciereszko, B. Piros, K. Dabrowski, D. Kucharczyk, M. J. \u0141uczy\u0144ski,\nS. Dobosz, and J. Glogowski, \"Serine proteinase inhibitors of seminal\nplasma of teleost fish: distribution of activity, electrophoretic profiles\nand relation to proteinase inhibitors of blood\". J.Fish Bio. 53: 1292-\n1305, 1998.\n[12] A. Ciereszko, T. W\u253c\u00e9asow, S. Dobosz, K. Goryczko and J. Glogowski,\n\"Blood cells in rainbow trout Oncorhynchus mykiss ilt: relation to milt\ncollection method and sampling period\". Theriogenology 62: 1353-1364,2004.\n[13] G. J. Dietrich, R. Kowalski, M. Wojtczak, S. Dobosz and K. Goryczko,\n\"Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post\nmortem storage and anesthesia\". Fish Physiol. Biochem. 31: 1-9, 2005b.\n[14] G. J. Dietrich, A. Szpyrka, M. Wojtczak, S. Dobosz, K. Goryczko, \u0141. \n\u017bakowski and A. Ciereszko, \"Effects of UV irradiation and hydrogen \nperoxide on DNA fragmentation, motility and fertilizing ability of \nrainbow trout (Oncorhynchus mykiss) spermatozoa\". Theriogenology 64: \n1809-1822, 2005a\n[15] E.M. Donaldson, \"Manipulation of reproduction in farmed fish.\" Anim \nReprod Sci 42: 381\u2013392, 1996. \n[16] C. Dreanno, M. Suquet, E. Desbruyeres, J. Cosson, H. Le Delliou, and \nR. Billard, \"Effect of urine on sperm quality in turbot (Psetta maxima)\". \nAquaculture 169(3\u20134): 247\u2013262, 1998.\n[17] K. Dziewulska and J. Domaga\u0142a, \"Histology of salmonid testis during \nmaturation\". Reprod. Biol. 3(1): 47-61, 2003. \n[18] J. L. Fitzpatrick, J. C. Henry, N. R. Liley and R. H. Devlin, \"Sperm \ncharacteristicks and fertilization succes of masculinized coho salmon \n(Oncorhynchus kisutch)\". Aquaculture 249: 459-468, 2005. \n[19] M. J. G. Gage, C. P. Macfarlane, S. Yeates, R. G. Ward, J. B. Searle and \nG. A. Parker, \"Spermatozoal traits and sperm competition in Atlantic \nsalmon: relative sperm velocity is the primary determinant of \nfertilization success\". Curr. Biol. 14: 44\u201347, 2004.\n[20] J. F. Galas, A. Hejmej, J. Glogowski and B. Bili\u0144ska, \"Morphological \nand functional alterations in testis and efferent ducts of homogametic \nrainbow trout Oncorhynchus mykiss Walbaum\". Ann N Y Acad Sci 1163: \n398\u2013401, 2009. \n[21] A. J. Geffen and J. P. Evans, \"Sperm traits and fertilization success of \nmale and sex-reversed female rainbow trout (Oncorhynchus mykiss)\". \nAquaculture 182: 61-72, 2000. \n[22] S.E. Hartley, \"The chromosomes of Salmonid fishes\". Biol. Rev. 62: \n197-214, 1987. \n[23] D. J. Hoysak and N. R. Liley. \"Fertilization dynamics in sockeye salmon \nand a comparison of sperm from alternative male phenotypes\". J. Fish \nBiol. 58: 1286-1300, 2000.\n[24] D. E. Kime, K. J. W. Van Look, B. G. McAllister, G. Huyskens, E. \nRurangwa and F. Ollevier , \"Computer-assisted sperm analysis (CASA) \nas a tool for monitoring sperm quality in fish\". Comp Biochem Physiol C \nPharmacol Toxicol Endocrinol 130: 425\u2013433, 2001. \n[25] K. Kinnberg, B. Korsgaard, P. Bjerregaard and A. Jespersen, \"Effects of \nnonylphenol and 17\u03b2-estradiol on vitellogenin synthesis and testis \nmorphology in male platyfish Xiphophorus maculates\". J. Exp. Biol.\n203: 171-181, 2000. \n[26] M. Koldras, M. Loir, G. Maisse and F. Le Gae, \"Study of the \ncomposition of the seminal fluid and of sperm motility along genital \ntract, during a spawning season, in the rainbow trout (Oncorhynchus \nmykiss) \". Aquat. Living Resour. 9(4): 337-345, 1996. \n[27] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner, \n\"Physiological and biochemical determination of rainbow trout, \nOncorhynchus mykiss, sperm quality for cryopreservation\". J. Appl. \nIchthyol. 6(4): 47-73, 1996. \n[28] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner, \n\"Determination of sperm quality of the rainbow trout, Oncorhynchus \nmykiss, by sperm motility, seminal plasma parameters, and spermatozoal \nmetabolism\". Aquaculture 163: 163-181, 1998. \n[29] F. Lahnsteiner, N. Mansour and B. Berger. \"Seminal plasma proteins \nprolong the viability of rainbow trout (Oncorynchus mykiss) \nspermatozoa\". Theriogenology 62: 801-808, 2004. \n[30] F. Lahnsteiner, R. A. Patzner and T. Weismann, \"Energy resources of \nspermatozoa of the rainbow trout Oncorhynchus mykiss (Pisces, \nTeleostei)\". Reprod. Nutr. Dev. 33: 349-360, 1993b. \n[31] F. Lahnsteiner, R. Patzner and T. Weismann, \"The spermatic ducts of \nSalmonid fishes (Salmonidae, Teleostei). Morphology, histochemistry \nand composition of the secretion\". J. Fish Biol. 42: 79-93, 1993a. \n[32] F. Lahnsteiner, R. Patzner and T. Weismann, \"The testicular main ducts \nand the spermatic ducts in some cyprinid fishes-II. Composition of the \nseminal fluid\". J. Fish Biol. 44: 459-467, 1994. \n[33] N. Mansour, A. Ramoun and F. Lahnsteiner, \"Quality of testicular sperm \nof the African catfish Clarias gariepinus (Burchell, 1822) and its \nrelationship with fertilization and hatching success\". Aquacult. Res. 36: \n1422-1428, 2005. \n[34] S. Morisawa and M. Morisawa, \"Acquisition of potential for sperm \nmotility in rainbow trout and chum salmon\". J. Exp. Biol. 126: 89-96, \n1986. \n[35] H. Ohta, T. Unuma, M. Yoshioka and M. Kashiwagi, \"Effects of \nbicarbonate ions and pH on acquisition and maintenance of potential for\nmotility in ayu, Plecoglossus altivelis Temminck et Schlegel \n(Osmeridae), spermatozoa\". Aquacult. Res. 32: 385-392, 2001. \n[36] A. Pecio, F. Lahnsteiner and J. Rafi\u0144ski, \"Ultrastucture of the epithelial \ncells in the aspermatogenic part of the testis in Mimagoniates barberi \n(Teleostei: Characidae: Glandulocaudinae) and the role of their \nsecretions in spermatozeugmata formation\". Ann. Anat. 183: 427-435, \n2001.\n[37] G. Perchec J. , Cosson, F. Andre and R. Billard, \"Degradation of the \nquality of carp sperm by urine contamination during stripping\". \nAquaculture 129: 135-136, 1995. \n[38] K. Ravinder, K. Nasaruddin, K. C. Majumdar and S. Shivaji, \n\"Computerized analysis of motility, motility patterns and motility \nparameters of spermatozoa of carp following short-term storage of \nsperm\". J. Fish Biol. 50: 1309-1328, 1997. \n[39] H. Schagger and G. von Jagow, \"Tricine-sodium dodecyl sulfate-\npolyacrylamide gel electrophoresis for the separation of proteins in the\nrange from 1 to 100 kDa\". Anal. Biochem. 166: 368-379, 1987.\n[40] A. P. Scott and S. M. Baynes, \"A review of the biology, handling and \nstorage of salmonid spermatozoa\". J. Fish Biol. 17: 707-739, 1980. \n[41] S. Shivaji, J. Peedicayil andD. L. Girija, \"Analysis of the motility \nparameters of in vitro hyperactivated hamster spermatozoa\". Mol.\nReprod. Dev. 42: 233-247, 1995"]}