Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase

In this paper, naturally immobilized lipase, Carica papaya lipase, catalyzed biodiesel production from fish oil was studied. The refined fish oil, extracted from the discarded parts of fish, was used as a starting material for biodiesel production. The effects of molar ratio of oil: methanol, lipase...

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Main Authors: P. Pinyaphong, P. Sriburi, S. Phutrakul
Format: Text
Language:English
Published: Zenodo 2011
Subjects:
Online Access:https://dx.doi.org/10.5281/zenodo.1071467
https://zenodo.org/record/1071467
id ftdatacite:10.5281/zenodo.1071467
record_format openpolar
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language English
topic biodiesel fuel production
methanolysis
fish oil
Carica papaya lipase.
spellingShingle biodiesel fuel production
methanolysis
fish oil
Carica papaya lipase.
P. Pinyaphong
P. Sriburi
S. Phutrakul
Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase
topic_facet biodiesel fuel production
methanolysis
fish oil
Carica papaya lipase.
description In this paper, naturally immobilized lipase, Carica papaya lipase, catalyzed biodiesel production from fish oil was studied. The refined fish oil, extracted from the discarded parts of fish, was used as a starting material for biodiesel production. The effects of molar ratio of oil: methanol, lipase dosage, initial water activity of lipase, temperature and solvent were investigated. It was found that Carica papaya lipase was suitable for methanolysis of fish oil to produce methyl ester. The maximum yield of methyl ester could reach up to 83% with the optimal reaction conditions: oil: methanol molar ratio of 1: 4, 20% (based on oil) of lipase, initial water activity of lipase at 0.23 and 20% (based on oil) of tert-butanol at 40oC after 18 h of reaction time. There was negligible loss in lipase activity even after repeated use for 30 cycles. : {"references": ["K. Annapurna, M. Paramita, G. K. Vijay and B. Rintu, \"Enzymatic\ntransesterification of Jatropha oil,\" Biotech& Biofuels, vol. 2, no page,\n2009.", "N. Kaili, X. Feng, W. Fang and T. Tianwei, \"Lipase catalyzed\nmethanolysis to produce biodiesel: optimization of biodiesel\nproduction,\" J. Mol. Catal. B: Enzym. Vol 43, 142-147, 2006.", "W. Hong, Z. Min-hua, L. Qian and W. Hua-chang, \"Enzymatic\nconversion of waste oil to biodiesel in a solvent-free system,\" Prepr.\nPap. Am. Chem. Soc., Div. Fuel Chem. Vol. 48, 533-534, 2003.", "R. Alcantara, J. Amores, L. Canoira, E. Fidalgo, M. J. Franco and A.\nNavarro, \"Catalytic production of biodiesel from soy-bean oil, used\nfrying oil and tallow,\" Biomass & Bioenergy. Vol 18, 515-527, 2000.", "A. Robles-Medina, P. A. Gonzalez-Moreno, L. Esteban-R Cerdan and E.\nMolina-Grima, \"Biocatalysis: Towards ever greener biodiesel\nproduction,\" Biotechnology Advances., vol. 27, pp. 398-408, 2009.", "L. Lilin, D. Wei, L. Dehua, W. Li and L. Zebo, \"Lipase-catalyzed\ntransesterification of rapeseed oils for biodiesel production with a novel\norganic solvent as the reaction medium,\" J. Mol. Catal. B: Enzym., vol.\n43, 58-62, 2006.", "G. Steinke, R. Kirchhoff and K.D. Mukherjee, \"Lipase-catalyzed\nalcoholysis of crambe oil and camelina oil for the preparation of longchain\nesters,\" J. Am. Oil Chem. Soc., vol. 69, 166-169, 2000.", "Y. Watanabe, Y. Shimada, A. Sugihara, H. Noda, H. Fukuda and Y.\nTominaga, \"Continuous production of biodiesel fuel from vegetable oil\nusing immobilized Candida antarctica lipase,\" J. Am. Oil Chem. Soc.,\nvol. 77, 355-360, 2000.", "W. Du, W. Li, T. Sun, X. Chen and D. Liu, \"Perspectives for\nbiotechnological production of biodiesel and impacts,\" Appl Microbiol\nBiotechnol, vol. 79, 331-337, 2008.\n[10] Y. Shimada, Y. Watanabe, A. Sugihara and Y. Tominaga, \"Enzymatic\nalcoholysis for biodiesel fuel production and application of the reaction\nto oil processing,\" J. Mol. Catal. B: Enzym., vol. 17, 133-142, 2002.\n[11] O. Kose, M. Tuter and H. A. Aksoy, \"Immobilized Candida Antarctica\nlipase-catalyzed alcoholysis of cotton seed oil in a solvent-free\nmedium,\" Bioresour. Technol., vol. 83, 125-129, 2002.\n[12] P. Villeneuve, A. Skarbek, M. Pina, J. Graille and T. A. Foglia,\n\"Catalytic behavior of Carica papaya latex in transesterification\nreactions,\" Biotechnol. Tech., vol. 11, 637-639, 1997.\n[13] M. Kaieda, T. Samukawa, T. Matsumoto, K. Ban, A. Kondo, Y.\nShimada, H. Noda, F. Nomoto, K. Ohtsuka, E. Izumoto and H. Fukuda,\n\"Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae\nlipase in a water containing system without an organic solvent,\" J.\nBiosci. Bioeng., vol. 88, 627-631, 1999.\n[14] K. Ban, M. Kaieda, T. Matsumoto, A. Kondo and H. Fukuda, \"Whole\ncell biocatalysts for biodiesel fuel production utilizing Rhizopus oryzae\ncells immobilized within biomass support particles,\" Biochem. Eng. J.,\nvol. 8, 39-43, 2001.\n[15] T. A. Foglia and P. Villeneuve, \"Carica papaya latex-catalyzed synthesis\nof structured triacylglycerol,\" J. Am. Oil Chem. Soc., vol. 74, 1447-\n1450, 1997.\n[16] [16] P. Angkanurukpun, P. Sriburi and P. Kanasawud, \"Improvement of\nCarica papaya lipase for methanolysis of triolein,\" Chiang Mai J. Sci.,\nvol. 33, 217-222, 2006.\n[17] L. Cherng-Yuan and L. Rong-Ji, \"Fuel properties of biodiesel produced\nfrom the crude fish oil from the soapstock of marine fish,\" Fuel\nProcessing Technology., 130-136, 2008.\n[18] D. Y. Kwon and J. S. Rhee, \"A simple and rapid colorimetric method for\ndetermination of free fatty acids for lipase assay,\" J. Am. Oil Chem.\nSoc., vol. 63, 89-92, 1986.\n[19] A. S. Ramadhas, S. Jayaraj and C. Muraleedharan, \"Biodiesel production\nfrom high FFA rubber seed oil,\" Fuel., Vol. 84, 335-340, 2005.\n[20] M. Canakci and J. G. Van, \"Biodiesel production from oils and fats with\nhigh free fatty acids,\" Transactions of the ASAE., vol. 44, 1429-1436,\n2001b.\n[21] M. Canakci and J. G. Van, \"A pilot plant to produce biodiesel from high\nfree fatty acid feedstocks,\" ASAE, 2001a.\n[22] K. Krisnangkura, T. Yimsuwan and R. Pairintra, \"An empirical approach\nin predicting biodiesel viscosity at various temperature,\" Fuel., vol. 85,\n107-113, 2006.\n[23] L. A. Nelson, T. A. Foglia and W. N. Marmer, \"Lipase catalyzed\nproduction of biodiesel,\" J. Am Oil Chem Soc., vol. 73, 1191-1195,\n1996.\n[24] V. Dossat, D. Combes and A. Marty, \"Continuous enzymatic\ntransesterification of high oleic sunflower oil in a packed bed reactor:\ninfluence of the glycerol production,\" Enzyme Microb Technol., vol. 25,\n194-200, 1999.\n[25] L. Jike, N. Kaili, X. Feng, W. Fang and T. Tianwei, \"Enzymatic\nsynthesis of fatty acid methyl esters from lard with immobilized Candida\nsp. 99-125,\" Process Biochemistry., vol. 42, 1367-1370, 2007."]}
format Text
author P. Pinyaphong
P. Sriburi
S. Phutrakul
author_facet P. Pinyaphong
P. Sriburi
S. Phutrakul
author_sort P. Pinyaphong
title Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase
title_short Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase
title_full Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase
title_fullStr Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase
title_full_unstemmed Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase
title_sort biodiesel fuel production by methanolysis of fish oil derived from the discarded parts of fish catalyzed by carica papaya lipase
publisher Zenodo
publishDate 2011
url https://dx.doi.org/10.5281/zenodo.1071467
https://zenodo.org/record/1071467
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geographic Chiang
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Gonzalez
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geographic_facet Chiang
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Gonzalez
Kondo
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Pina
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genre Antarc*
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genre_facet Antarc*
Antarctica
op_relation https://dx.doi.org/10.5281/zenodo.1071468
op_rights Open Access
Creative Commons Attribution 4.0
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info:eu-repo/semantics/openAccess
op_rightsnorm CC-BY
op_doi https://doi.org/10.5281/zenodo.1071467
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spelling ftdatacite:10.5281/zenodo.1071467 2023-05-15T14:00:10+02:00 Biodiesel Fuel Production By Methanolysis Of Fish Oil Derived From The Discarded Parts Of Fish Catalyzed By Carica Papaya Lipase P. Pinyaphong P. Sriburi S. Phutrakul 2011 https://dx.doi.org/10.5281/zenodo.1071467 https://zenodo.org/record/1071467 en eng Zenodo https://dx.doi.org/10.5281/zenodo.1071468 Open Access Creative Commons Attribution 4.0 https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess CC-BY biodiesel fuel production methanolysis fish oil Carica papaya lipase. Text Journal article article-journal ScholarlyArticle 2011 ftdatacite https://doi.org/10.5281/zenodo.1071467 https://doi.org/10.5281/zenodo.1071468 2021-11-05T12:55:41Z In this paper, naturally immobilized lipase, Carica papaya lipase, catalyzed biodiesel production from fish oil was studied. The refined fish oil, extracted from the discarded parts of fish, was used as a starting material for biodiesel production. The effects of molar ratio of oil: methanol, lipase dosage, initial water activity of lipase, temperature and solvent were investigated. It was found that Carica papaya lipase was suitable for methanolysis of fish oil to produce methyl ester. The maximum yield of methyl ester could reach up to 83% with the optimal reaction conditions: oil: methanol molar ratio of 1: 4, 20% (based on oil) of lipase, initial water activity of lipase at 0.23 and 20% (based on oil) of tert-butanol at 40oC after 18 h of reaction time. There was negligible loss in lipase activity even after repeated use for 30 cycles. : {"references": ["K. Annapurna, M. Paramita, G. K. Vijay and B. Rintu, \"Enzymatic\ntransesterification of Jatropha oil,\" Biotech& Biofuels, vol. 2, no page,\n2009.", "N. Kaili, X. Feng, W. Fang and T. Tianwei, \"Lipase catalyzed\nmethanolysis to produce biodiesel: optimization of biodiesel\nproduction,\" J. Mol. Catal. B: Enzym. Vol 43, 142-147, 2006.", "W. Hong, Z. Min-hua, L. Qian and W. Hua-chang, \"Enzymatic\nconversion of waste oil to biodiesel in a solvent-free system,\" Prepr.\nPap. Am. Chem. Soc., Div. Fuel Chem. Vol. 48, 533-534, 2003.", "R. Alcantara, J. Amores, L. Canoira, E. Fidalgo, M. J. Franco and A.\nNavarro, \"Catalytic production of biodiesel from soy-bean oil, used\nfrying oil and tallow,\" Biomass & Bioenergy. Vol 18, 515-527, 2000.", "A. Robles-Medina, P. A. Gonzalez-Moreno, L. Esteban-R Cerdan and E.\nMolina-Grima, \"Biocatalysis: Towards ever greener biodiesel\nproduction,\" Biotechnology Advances., vol. 27, pp. 398-408, 2009.", "L. Lilin, D. Wei, L. Dehua, W. Li and L. Zebo, \"Lipase-catalyzed\ntransesterification of rapeseed oils for biodiesel production with a novel\norganic solvent as the reaction medium,\" J. Mol. Catal. B: Enzym., vol.\n43, 58-62, 2006.", "G. Steinke, R. Kirchhoff and K.D. Mukherjee, \"Lipase-catalyzed\nalcoholysis of crambe oil and camelina oil for the preparation of longchain\nesters,\" J. Am. Oil Chem. Soc., vol. 69, 166-169, 2000.", "Y. Watanabe, Y. Shimada, A. Sugihara, H. Noda, H. Fukuda and Y.\nTominaga, \"Continuous production of biodiesel fuel from vegetable oil\nusing immobilized Candida antarctica lipase,\" J. Am. Oil Chem. Soc.,\nvol. 77, 355-360, 2000.", "W. Du, W. Li, T. Sun, X. Chen and D. Liu, \"Perspectives for\nbiotechnological production of biodiesel and impacts,\" Appl Microbiol\nBiotechnol, vol. 79, 331-337, 2008.\n[10] Y. Shimada, Y. Watanabe, A. Sugihara and Y. Tominaga, \"Enzymatic\nalcoholysis for biodiesel fuel production and application of the reaction\nto oil processing,\" J. Mol. Catal. B: Enzym., vol. 17, 133-142, 2002.\n[11] O. Kose, M. Tuter and H. A. Aksoy, \"Immobilized Candida Antarctica\nlipase-catalyzed alcoholysis of cotton seed oil in a solvent-free\nmedium,\" Bioresour. Technol., vol. 83, 125-129, 2002.\n[12] P. Villeneuve, A. Skarbek, M. Pina, J. Graille and T. A. Foglia,\n\"Catalytic behavior of Carica papaya latex in transesterification\nreactions,\" Biotechnol. Tech., vol. 11, 637-639, 1997.\n[13] M. Kaieda, T. Samukawa, T. Matsumoto, K. Ban, A. Kondo, Y.\nShimada, H. Noda, F. Nomoto, K. Ohtsuka, E. Izumoto and H. Fukuda,\n\"Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae\nlipase in a water containing system without an organic solvent,\" J.\nBiosci. Bioeng., vol. 88, 627-631, 1999.\n[14] K. Ban, M. Kaieda, T. Matsumoto, A. Kondo and H. Fukuda, \"Whole\ncell biocatalysts for biodiesel fuel production utilizing Rhizopus oryzae\ncells immobilized within biomass support particles,\" Biochem. Eng. J.,\nvol. 8, 39-43, 2001.\n[15] T. A. Foglia and P. Villeneuve, \"Carica papaya latex-catalyzed synthesis\nof structured triacylglycerol,\" J. Am. Oil Chem. Soc., vol. 74, 1447-\n1450, 1997.\n[16] [16] P. Angkanurukpun, P. Sriburi and P. Kanasawud, \"Improvement of\nCarica papaya lipase for methanolysis of triolein,\" Chiang Mai J. Sci.,\nvol. 33, 217-222, 2006.\n[17] L. Cherng-Yuan and L. Rong-Ji, \"Fuel properties of biodiesel produced\nfrom the crude fish oil from the soapstock of marine fish,\" Fuel\nProcessing Technology., 130-136, 2008.\n[18] D. Y. Kwon and J. S. Rhee, \"A simple and rapid colorimetric method for\ndetermination of free fatty acids for lipase assay,\" J. Am. Oil Chem.\nSoc., vol. 63, 89-92, 1986.\n[19] A. S. Ramadhas, S. Jayaraj and C. Muraleedharan, \"Biodiesel production\nfrom high FFA rubber seed oil,\" Fuel., Vol. 84, 335-340, 2005.\n[20] M. Canakci and J. G. Van, \"Biodiesel production from oils and fats with\nhigh free fatty acids,\" Transactions of the ASAE., vol. 44, 1429-1436,\n2001b.\n[21] M. Canakci and J. G. Van, \"A pilot plant to produce biodiesel from high\nfree fatty acid feedstocks,\" ASAE, 2001a.\n[22] K. Krisnangkura, T. Yimsuwan and R. Pairintra, \"An empirical approach\nin predicting biodiesel viscosity at various temperature,\" Fuel., vol. 85,\n107-113, 2006.\n[23] L. A. Nelson, T. A. Foglia and W. N. Marmer, \"Lipase catalyzed\nproduction of biodiesel,\" J. Am Oil Chem Soc., vol. 73, 1191-1195,\n1996.\n[24] V. Dossat, D. Combes and A. Marty, \"Continuous enzymatic\ntransesterification of high oleic sunflower oil in a packed bed reactor:\ninfluence of the glycerol production,\" Enzyme Microb Technol., vol. 25,\n194-200, 1999.\n[25] L. Jike, N. Kaili, X. Feng, W. Fang and T. Tianwei, \"Enzymatic\nsynthesis of fatty acid methyl esters from lard with immobilized Candida\nsp. 99-125,\" Process Biochemistry., vol. 42, 1367-1370, 2007."]} Text Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) Chiang ENVELOPE(162.650,162.650,-77.967,-77.967) Fang ENVELOPE(167.217,167.217,-77.483,-77.483) Gonzalez ENVELOPE(-58.250,-58.250,-63.917,-63.917) Kondo ENVELOPE(161.847,161.847,55.716,55.716) Medina ENVELOPE(-66.233,-66.233,-68.453,-68.453) Moreno ENVELOPE(-62.300,-62.300,-64.083,-64.083) Pina ENVELOPE(6.797,6.797,62.685,62.685) Robles ENVELOPE(-61.450,-61.450,-64.367,-64.367)