Data from: Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. ...

• Main Authors: Nualart, Daniela P., Dann, Francisco, Oyarzún-Salazar, Ricardo, Morera, Francisco J., Vargas-Chacoff, Luis
• Format: Dataset
• Language: English
• Published: Zenodo 2023
• Subjects: immune response; head kidney cells; primary culture; LPS; POLY IC; salmonids; Salmo salar
• Online Access: https://dx.doi.org/10.5281/zenodo.10436721; https://zenodo.org/doi/10.5281/zenodo.10436721

Data from Immune Transcriptional Response in Head Kidney Primary Cell Cultures Isolated from the Three Most Important Species in Chilean Salmonids Aquaculture. Files in .JBN and .xlsx format ... : Fish cell culture is a common in vitro tool for studies in different fields such as virology, toxicology, pathology and immunology of fish. Fish cell cultures are a promising help to study how to diagnose and control relevant viral and intracellular bacterial infections in aquaculture. They can also be used for developing vaccines and immunostimulants, especially with the ethical demand aiming to reduce and replace the number of fish used in research. This study aimed to isolate head kidney primary cell cultures from three Chilean salmonids: Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss, and characterize the response to bacterial and viral stimuli by evaluating various markers of the innate and adaptive immune response. Specifically, the primary cell cultures of the head kidney from the three salmonids studied were cultured and exposed to two substances that mimic molecular patterns of different pathogens, i.e., Lipopolysaccharide (LPS) (bacterial) and Polyinosinic: polycytidylic acid (POLY ...