Selective lipid recruitment by an archaeal DPANN symbiont from its host ...
Abstract The symbiont Ca. Nanohaloarchaeum antarcticus is obligately dependent on its host Halorubrum lacusprofundi for lipids and other metabolites due to its lack of certain biosynthetic genes. However, it remains unclear which specific lipids or metabolites are acquired from its host, and how the...
| Main Authors: | , , , , |
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| Format: | Dataset |
| Language: | unknown |
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Zenodo
2023
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5281/zenodo.10258364 https://zenodo.org/doi/10.5281/zenodo.10258364 |
| Summary: | Abstract The symbiont Ca. Nanohaloarchaeum antarcticus is obligately dependent on its host Halorubrum lacusprofundi for lipids and other metabolites due to its lack of certain biosynthetic genes. However, it remains unclear which specific lipids or metabolites are acquired from its host, and how the host responds to infection. Here, we explored the lipidome dynamics of the Ca. Nha. antarcticus – Hrr. lacusprofundi symbiotic relationship during co-cultivation. By using a comprehensive untargeted lipidomic methodology, our study reveals that Ca. Nha. antarcticus selectively recruits 110 lipid species from its host, i.e. nearly two-thirds of the total number of host lipids. Lipid profiles of co-cultures displayed shifts in abundances of bacterioruberins and menaquinones and changes in the degree of bilayer-forming glycerolipid unsaturation. This likely results in increased membrane fluidity and improved resistance to membrane disruptions, consistent with compensation for higher metabolic load and mechanical ... : SD and JH contributed equally to this work. JSSD received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement no.694569—MICROLIPIDS) and from a Spinoza award from NWO. AS has received funding from the NWO-I foundation of the Netherlands Organisation for Scientific Research (WISE fellowship to AS), the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No. 947317, ASymbEL), the Moore–Simons Project on the Origin of the Eukaryotic Cell, Simons Foundation 735929LPI (https://doi.org/10.46714/735929LPI), and a Gordon and Betty Moore Foundation's Symbiosis in Aquatic Systems Initiative (GBMF9346). We thank Michel Koenen for the lipidome extraction. We acknowledge Wencong Huang and Dina Castillo Boukhchtaber for their support in the culturing experiment and Prof. Stefan Schouten, Prof. Laura Villanueva, Dr. Kerstin Fiege and Dr. Diana Sahonero Canavesi who ... |
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