Microchamber slide design for cell confinement during imaging- Tetrahymena rostrata timelapse data ...

• Main Author: Garcia III, Galo
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Zenodo 2023
• Subjects: Orca
• Online Access: https://dx.doi.org/10.5281/zenodo.10138858; https://zenodo.org/doi/10.5281/zenodo.10138858

We performed Imaging on a Nikon Ti2-E & Yokogawa CSU W1-SoRa microscope. The microscope was equipped with an ORCA-Fusion BT digital C-MOS camera. We used a 10× 0.45 Plan Apo Air or a Plan Apo λD 100x OIL OFN25 DIC N2 objective for differential interference contrast (DIC) imaging. More microscopy information is detailed in the metadata file associated with each .nd2 file.Tetrahymena rostrata was provided by Andrzej Kaczanowski, who isolated the cells from the small snail Cohlicopa lubrica near Warsaw. The cells were cultured in medium consisting 0.5% yeast extract and 0.5% of proteose peptone supplemented with 250 ug/ml streptomycin sulphate and 250 ug/ml penicillin G to maintain sterility. ...