Detection of the Aquatic Invasive Species (AIS) Elodea canadensis and E. nuttallii Using Environmental DNA (eDNA), Alaska ...

• Main Author: Damian M Menning
• Format: Dataset
• Language: unknown
• Published: U.S. Geological Survey 2024
• Subjects: Wildlife; Coastal ecosystems; Genetics; FOS Biological sciences; Environment; Biota; Aquatic ecosystems; Wetland ecosystems; Polymerase chain reaction; DNA sequencing; DNA Primers; Genetic markers; Marine environment monitoring; Alaska; Weeds, noxious plants, or invasive plants; Aquatic invasive species; Mitochondrial DNA; Tundra ecosystems; Environmental DNA; eDNA; 5.8S; atpB-rbcL; trnL-trnF; Elodea; Metabarcoding; Tundra
• Online Access: https://dx.doi.org/10.5066/p94zaugk; https://www.sciencebase.gov/catalog/item/663d2da7d34eaf9729f78cc9

This data set is comprised of one table with sampling information and National Center for Biotechnology Information (NCBI) BioProject accession numbers for sequence information of this amplicon-based study targeting Elodea canadensis and E. nuttallii in freshwater systems of Alaska from environmental samples. Highly conserved primers which can differentiate these species of interest were developed for four portions of Elodea mtDNA genes (ITS1-5.8S, atpB-rbcL, and two variations of trnL-trnF). The reference sequences and conserved primer sets to identify species present were developed using publicly available data from NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/). ...