Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington
Dataset for the publication 'Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington'. This research was initiated in conjunction with a systematic, multi-ag...
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U.S. Geological Survey
2018
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Online Access: | https://dx.doi.org/10.5066/f7vx0f1h https://www.sciencebase.gov/catalog/item/59a5a97fe4b0b0bfe810d952 |
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ftdatacite:10.5066/f7vx0f1h 2023-05-15T15:32:41+02:00 Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington Purcell, Maureen K. Powers, Rachel L. 2018 https://dx.doi.org/10.5066/f7vx0f1h https://www.sciencebase.gov/catalog/item/59a5a97fe4b0b0bfe810d952 unknown U.S. Geological Survey https://dx.doi.org/https://doi.org/10.1111/jfd.12740 Diagnostic testing dataset Dataset 2018 ftdatacite https://doi.org/10.5066/f7vx0f1h https://doi.org/https://doi.org/10.1111/jfd.12740 2021-11-05T12:55:41Z Dataset for the publication 'Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington'. This research was initiated in conjunction with a systematic, multi-agency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon anemia virus (ISAV) RNA in British Columbia, Canada. In the systematic surveillance study reported in a companion paper, tissues from various salmonids taken from Washington and Alaska were surveyed for ISAV RNA using the U.S. approved diagnostic method and samples were released for use in this present study only after testing negative. Here we tested a subset of these samples for ISAV RNA with three additional published molecular assays, as well as for RNA from salmonid alphavirus (SAV), piscine myocarditis virus (PMCV) and piscine orthoreovirus (PRV). All samples (n=2252; 121 stock cohorts) tested negative for RNA from ISAV, PMCV, and SAV. In contrast, there were 25 stock cohorts from Washington and Alaska that had one or more individuals test positive for PRV RNA; prevalence within stocks varied and ranged from 2 73%. The overall prevalence of PRV RNA positive individuals across the study was 3.4% (77 of 2252 fish tested). Findings of PRV RNA were most common in coho (Oncorhynchus kisutch Walbaum) and Chinook (O. tshawytscha Walbaum) salmon. The disease associated with PRV in Atlantic salmon, heart skeletal muscle inflammation (HSMI), has not been reported in Pacific salmon. Dataset Atlantic salmon Alaska DataCite Metadata Store (German National Library of Science and Technology) British Columbia ENVELOPE(-125.003,-125.003,54.000,54.000) Canada Pacific Sav’ ENVELOPE(156.400,156.400,68.817,68.817) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Diagnostic testing |
spellingShingle |
Diagnostic testing Purcell, Maureen K. Powers, Rachel L. Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington |
topic_facet |
Diagnostic testing |
description |
Dataset for the publication 'Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington'. This research was initiated in conjunction with a systematic, multi-agency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon anemia virus (ISAV) RNA in British Columbia, Canada. In the systematic surveillance study reported in a companion paper, tissues from various salmonids taken from Washington and Alaska were surveyed for ISAV RNA using the U.S. approved diagnostic method and samples were released for use in this present study only after testing negative. Here we tested a subset of these samples for ISAV RNA with three additional published molecular assays, as well as for RNA from salmonid alphavirus (SAV), piscine myocarditis virus (PMCV) and piscine orthoreovirus (PRV). All samples (n=2252; 121 stock cohorts) tested negative for RNA from ISAV, PMCV, and SAV. In contrast, there were 25 stock cohorts from Washington and Alaska that had one or more individuals test positive for PRV RNA; prevalence within stocks varied and ranged from 2 73%. The overall prevalence of PRV RNA positive individuals across the study was 3.4% (77 of 2252 fish tested). Findings of PRV RNA were most common in coho (Oncorhynchus kisutch Walbaum) and Chinook (O. tshawytscha Walbaum) salmon. The disease associated with PRV in Atlantic salmon, heart skeletal muscle inflammation (HSMI), has not been reported in Pacific salmon. |
format |
Dataset |
author |
Purcell, Maureen K. Powers, Rachel L. |
author_facet |
Purcell, Maureen K. Powers, Rachel L. |
author_sort |
Purcell, Maureen K. |
title |
Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington |
title_short |
Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington |
title_full |
Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington |
title_fullStr |
Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington |
title_full_unstemmed |
Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington |
title_sort |
final dataset: molecular testing of adult pacific salmon and trout (oncorhynchus spp.) for several rna viruses demonstrates widespread distribution of piscine orthoreovirus (prv) in alaska and washington |
publisher |
U.S. Geological Survey |
publishDate |
2018 |
url |
https://dx.doi.org/10.5066/f7vx0f1h https://www.sciencebase.gov/catalog/item/59a5a97fe4b0b0bfe810d952 |
long_lat |
ENVELOPE(-125.003,-125.003,54.000,54.000) ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
British Columbia Canada Pacific Sav’ |
geographic_facet |
British Columbia Canada Pacific Sav’ |
genre |
Atlantic salmon Alaska |
genre_facet |
Atlantic salmon Alaska |
op_relation |
https://dx.doi.org/https://doi.org/10.1111/jfd.12740 |
op_doi |
https://doi.org/10.5066/f7vx0f1h https://doi.org/https://doi.org/10.1111/jfd.12740 |
_version_ |
1766363171009855488 |