Rare, long-distance dispersal underpins genetic connectivity in the pink sea fan, Eunicella verrucosa ...
Characterising patterns of genetic connectivity in marine species is of critical importance given the anthropogenic pressures placed on the marine environment. For sessile species, population connectivity can be shaped by many processes, such as pelagic larval duration, oceanographic boundaries, and...
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| Format: | Dataset |
| Language: | English |
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Dryad
2024
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| Online Access: | https://dx.doi.org/10.5061/dryad.xwdbrv1m9 https://datadryad.org/stash/dataset/doi:10.5061/dryad.xwdbrv1m9 |
| Summary: | Characterising patterns of genetic connectivity in marine species is of critical importance given the anthropogenic pressures placed on the marine environment. For sessile species, population connectivity can be shaped by many processes, such as pelagic larval duration, oceanographic boundaries, and currents. This study combines restriction-site associated DNA sequencing (RADseq) and passive particle dispersal modelling to delineate patterns of population connectivity in the pink sea fan, Eunicella verrucosa, a temperate octocoral. Individuals were sampled from 20 sites covering most of the species’ northeast Atlantic range, and a site in the northwest Mediterranean Sea to inform on connectivity across the Atlantic-Mediterranean transition. Using 7,510 neutral SNPs, a geographic cline of genetic clusters was detected, partitioning into: Ireland, Britain, France, Spain (Atlantic), and Portugal and Spain (Mediterranean). Evidence of significant inbreeding was detected at all sites, a finding not detected in a ... : Pink sea fan tissue samples (n = 285) were collected via SCUBA (under sampling license L/2019/00143 granted by the Marine Management Organisation) from depths ranging between 5 and 35 m between 2007-2019. In brief, apical 5 cm cuttings were removed from each sea fan colony underwater and preserved in >95% ethanol immediately after surfacing to prevent DNA degradation. Samples were stored long-term at 4 oC. Genomic DNA was extracted from polyp tissue using a salting-out protocol optimised for gorgonin-protein tissue (see Supplementary Material associated with the manuscript for full protocol). Sequencing and SNP isolation were performed through nextRAD, a reduced representation sequencing method. Genomic DNA was first fragmented with Nextera reagent (Illumina, Inc), which also ligates short adapter sequences to the ends of the fragments. The Nextera reaction was scaled for fragmenting 5 ng of genomic DNA, although 7.5 ng of genomic DNA was used for input to compensate for degraded DNA in the samples and to ... |
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