Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ...
Land use change can elevate disease risk by creating conditions beneficial to species that carry zoonotic pathogens. Observations of concordant global trends in pathogen prevalence and disease incidence have engendered concerns that urbanization could increase transmission risk of some pathogens. Ye...
Main Authors: | , , , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
Dryad
2020
|
Subjects: | |
Online Access: | https://dx.doi.org/10.5061/dryad.x95x69pgc https://datadryad.org/stash/dataset/doi:10.5061/dryad.x95x69pgc |
id |
ftdatacite:10.5061/dryad.x95x69pgc |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.5061/dryad.x95x69pgc 2024-06-09T07:49:14+00:00 Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ... Peterson, Anna Blum, Michael Ghersi, Bruno Riegel, Claudia Wunder, Elsio Childs, James 2020 https://dx.doi.org/10.5061/dryad.x95x69pgc https://datadryad.org/stash/dataset/doi:10.5061/dryad.x95x69pgc en eng Dryad https://www.ncbi.nlm.nih.gov/nuccore/MT816511%20-%20MT81679 https://dx.doi.org/10.1016/j.landurbplan.2019.103710 https://www.ncbi.nlm.nih.gov/nuccore/MT816511%20-%20MT81679 Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Rattus norvegicus Rattus rattus Mus musculus Leptospira interrogans Leptospira borgpetersenii Leptospira kirschneri counter-urbanization Dataset dataset 2020 ftdatacite https://doi.org/10.5061/dryad.x95x69pgc10.1016/j.landurbplan.2019.103710 2024-05-13T11:10:23Z Land use change can elevate disease risk by creating conditions beneficial to species that carry zoonotic pathogens. Observations of concordant global trends in pathogen prevalence and disease incidence have engendered concerns that urbanization could increase transmission risk of some pathogens. Yet host-pathogen relationships underlying transmission risk have not been well characterized within cities, even where contact between humans and species capable of transmitting pathogens of concern occur. We addressed this deficit by testing the hypothesis that areas in cities experiencing greater population loss and infrastructure decline (i.e., counter-urbanization) can support a greater diversity of host species and a larger and more diverse pool of pathogens. We did so by characterizing pathogenic Leptospira infection relative to rodent host richness and abundance across a mosaic of abandonment in post-Katrina New Orleans (Louisiana, USA). We found that Leptospira infection loads were highest in areas that ... : All animals were collected in the field using live trapping as also outlined in https://doi.org/10.1016/j.landurbplan.2019.103710. Tissue samples were collected immediately at necropsy and frozen at -80C. To identify pathogenic Leptospira to species, we sequenced a partial region of the glmU gene using genomic kidney DNA from all individuals that tested positive for Leptospira infection in the qPCR screen. After PCR amplification following a standard protocol, we cleaned all PCR products with ExoSAP-IT (Affymetrix, Santa Clara, CA, USA) and completed final paired sequencing reactions consisting of 3.75 µL PCR grade H20, 3.75 µL 5uM MgCl2, 1.0 µL each of 10 mM forward and reverse glmU primers (respectively) and 0.5 µL BigDye terminator (Applied Biosystems, Foster, CA, USA). We cleaned reactions using Sephadex columns prior to electrophoresis on an ABI 3730xl (Applied Biosystems). We aligned trimmed sequences with GenBank archived sequences of the glmU gene for all pathogenic Leptospira. We then constructed ... Dataset Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) Orleans ENVELOPE(-60.667,-60.667,-63.950,-63.950) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Rattus norvegicus Rattus rattus Mus musculus Leptospira interrogans Leptospira borgpetersenii Leptospira kirschneri counter-urbanization |
spellingShingle |
Rattus norvegicus Rattus rattus Mus musculus Leptospira interrogans Leptospira borgpetersenii Leptospira kirschneri counter-urbanization Peterson, Anna Blum, Michael Ghersi, Bruno Riegel, Claudia Wunder, Elsio Childs, James Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ... |
topic_facet |
Rattus norvegicus Rattus rattus Mus musculus Leptospira interrogans Leptospira borgpetersenii Leptospira kirschneri counter-urbanization |
description |
Land use change can elevate disease risk by creating conditions beneficial to species that carry zoonotic pathogens. Observations of concordant global trends in pathogen prevalence and disease incidence have engendered concerns that urbanization could increase transmission risk of some pathogens. Yet host-pathogen relationships underlying transmission risk have not been well characterized within cities, even where contact between humans and species capable of transmitting pathogens of concern occur. We addressed this deficit by testing the hypothesis that areas in cities experiencing greater population loss and infrastructure decline (i.e., counter-urbanization) can support a greater diversity of host species and a larger and more diverse pool of pathogens. We did so by characterizing pathogenic Leptospira infection relative to rodent host richness and abundance across a mosaic of abandonment in post-Katrina New Orleans (Louisiana, USA). We found that Leptospira infection loads were highest in areas that ... : All animals were collected in the field using live trapping as also outlined in https://doi.org/10.1016/j.landurbplan.2019.103710. Tissue samples were collected immediately at necropsy and frozen at -80C. To identify pathogenic Leptospira to species, we sequenced a partial region of the glmU gene using genomic kidney DNA from all individuals that tested positive for Leptospira infection in the qPCR screen. After PCR amplification following a standard protocol, we cleaned all PCR products with ExoSAP-IT (Affymetrix, Santa Clara, CA, USA) and completed final paired sequencing reactions consisting of 3.75 µL PCR grade H20, 3.75 µL 5uM MgCl2, 1.0 µL each of 10 mM forward and reverse glmU primers (respectively) and 0.5 µL BigDye terminator (Applied Biosystems, Foster, CA, USA). We cleaned reactions using Sephadex columns prior to electrophoresis on an ABI 3730xl (Applied Biosystems). We aligned trimmed sequences with GenBank archived sequences of the glmU gene for all pathogenic Leptospira. We then constructed ... |
format |
Dataset |
author |
Peterson, Anna Blum, Michael Ghersi, Bruno Riegel, Claudia Wunder, Elsio Childs, James |
author_facet |
Peterson, Anna Blum, Michael Ghersi, Bruno Riegel, Claudia Wunder, Elsio Childs, James |
author_sort |
Peterson, Anna |
title |
Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ... |
title_short |
Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ... |
title_full |
Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ... |
title_fullStr |
Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ... |
title_full_unstemmed |
Pathogenic Leptospira isolated from rodents in New Orleans, Louisiana USA, and associated site information ... |
title_sort |
pathogenic leptospira isolated from rodents in new orleans, louisiana usa, and associated site information ... |
publisher |
Dryad |
publishDate |
2020 |
url |
https://dx.doi.org/10.5061/dryad.x95x69pgc https://datadryad.org/stash/dataset/doi:10.5061/dryad.x95x69pgc |
long_lat |
ENVELOPE(-60.667,-60.667,-63.950,-63.950) |
geographic |
Orleans |
geographic_facet |
Orleans |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://www.ncbi.nlm.nih.gov/nuccore/MT816511%20-%20MT81679 https://dx.doi.org/10.1016/j.landurbplan.2019.103710 https://www.ncbi.nlm.nih.gov/nuccore/MT816511%20-%20MT81679 |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.x95x69pgc10.1016/j.landurbplan.2019.103710 |
_version_ |
1801381608192737280 |