A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ...
The major histocompatibility complex (MHC) is a highly polymorphic gene family that is crucial in immunity, and its diversity can be effectively used as a fitness marker for populations. Despite this, MHC remains poorly characterised in non-model species (e.g., cetaceans: whales, dolphins and porpoi...
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Online Access: | https://dx.doi.org/10.5061/dryad.wh70rxwvb https://datadryad.org/stash/dataset/doi:10.5061/dryad.wh70rxwvb |
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ftdatacite:10.5061/dryad.wh70rxwvb 2024-06-09T07:46:37+00:00 A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ... Heimeier, Dorothea Garland, Ellen Carroll, Emma Eichenberger, Franca Garrigue, Claire Vella, Adriana Baker, Scott 2023 https://dx.doi.org/10.5061/dryad.wh70rxwvb https://datadryad.org/stash/dataset/doi:10.5061/dryad.wh70rxwvb en eng Dryad Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 FOS Biological sciences Major histocompatibility complex cetacean MHC evolution MHC organisation humpback whale southern right whale Dataset dataset 2023 ftdatacite https://doi.org/10.5061/dryad.wh70rxwvb 2024-05-13T11:16:38Z The major histocompatibility complex (MHC) is a highly polymorphic gene family that is crucial in immunity, and its diversity can be effectively used as a fitness marker for populations. Despite this, MHC remains poorly characterised in non-model species (e.g., cetaceans: whales, dolphins and porpoises) as high gene copy number variation, especially in the fast-evolving class I region, makes analyses of genomic sequences difficult. To date, only small sections of class I and IIa genes have been used to assess functional diversity in cetacean populations. Here, we undertook a systematic characterisation of the MHC class I and IIa regions in available cetacean genomes. We extracted full-length gene sequences to design pan-cetacean primers that amplified the complete exon2 from MHC class I and IIa genes in one combined sequencing panel. We validated this panel in 19 cetacean species and described 354 alleles for both classes. Furthermore, we identified likely assembly artefacts for many MHC class I assemblies ... : A total of 85 tissue samples were taken from individual animals across several cetacean species. The type of tissue were either from strandings or biopsies. Stranding samples in New Zealand were taken by the Department of Conservation New Zealand and sent to the New Zealand Cetacean Tissue Archive (NZCeTA) housed at the University of Auckland Waipapa Taumata Rau with approval from mana whenua (Māori indigenous groups). Biopsy samples from New Zealand cetaceans include two Hector’s dolphin (Chephalorhyncus hectori) (Hamner et al., 2017) and two bottlenose dolphins (T. truncatus) (Tezanos-Pinto et al., 2009). Further biopsies include two rough-toothed dolphins (Steno bredanensis) and two Blainville beaked whales (Mesoplodon densirostris) from French-Polynesia (Albertson et al., 2017; Oremus et al., 2012). Details on samples and associated permit numbers can be found in the published manuscript. DNA was extracted from tissue samples and genomic DNA underwent PCR for five Major Histocompatibility Complex loci. ... Dataset Humpback Whale Southern Right Whale DataCite Metadata Store (German National Library of Science and Technology) New Zealand |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
FOS Biological sciences Major histocompatibility complex cetacean MHC evolution MHC organisation humpback whale southern right whale |
spellingShingle |
FOS Biological sciences Major histocompatibility complex cetacean MHC evolution MHC organisation humpback whale southern right whale Heimeier, Dorothea Garland, Ellen Carroll, Emma Eichenberger, Franca Garrigue, Claire Vella, Adriana Baker, Scott A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ... |
topic_facet |
FOS Biological sciences Major histocompatibility complex cetacean MHC evolution MHC organisation humpback whale southern right whale |
description |
The major histocompatibility complex (MHC) is a highly polymorphic gene family that is crucial in immunity, and its diversity can be effectively used as a fitness marker for populations. Despite this, MHC remains poorly characterised in non-model species (e.g., cetaceans: whales, dolphins and porpoises) as high gene copy number variation, especially in the fast-evolving class I region, makes analyses of genomic sequences difficult. To date, only small sections of class I and IIa genes have been used to assess functional diversity in cetacean populations. Here, we undertook a systematic characterisation of the MHC class I and IIa regions in available cetacean genomes. We extracted full-length gene sequences to design pan-cetacean primers that amplified the complete exon2 from MHC class I and IIa genes in one combined sequencing panel. We validated this panel in 19 cetacean species and described 354 alleles for both classes. Furthermore, we identified likely assembly artefacts for many MHC class I assemblies ... : A total of 85 tissue samples were taken from individual animals across several cetacean species. The type of tissue were either from strandings or biopsies. Stranding samples in New Zealand were taken by the Department of Conservation New Zealand and sent to the New Zealand Cetacean Tissue Archive (NZCeTA) housed at the University of Auckland Waipapa Taumata Rau with approval from mana whenua (Māori indigenous groups). Biopsy samples from New Zealand cetaceans include two Hector’s dolphin (Chephalorhyncus hectori) (Hamner et al., 2017) and two bottlenose dolphins (T. truncatus) (Tezanos-Pinto et al., 2009). Further biopsies include two rough-toothed dolphins (Steno bredanensis) and two Blainville beaked whales (Mesoplodon densirostris) from French-Polynesia (Albertson et al., 2017; Oremus et al., 2012). Details on samples and associated permit numbers can be found in the published manuscript. DNA was extracted from tissue samples and genomic DNA underwent PCR for five Major Histocompatibility Complex loci. ... |
format |
Dataset |
author |
Heimeier, Dorothea Garland, Ellen Carroll, Emma Eichenberger, Franca Garrigue, Claire Vella, Adriana Baker, Scott |
author_facet |
Heimeier, Dorothea Garland, Ellen Carroll, Emma Eichenberger, Franca Garrigue, Claire Vella, Adriana Baker, Scott |
author_sort |
Heimeier, Dorothea |
title |
A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ... |
title_short |
A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ... |
title_full |
A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ... |
title_fullStr |
A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ... |
title_full_unstemmed |
A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies ... |
title_sort |
pan-cetacean mhc amplicon sequencing panel developed and evaluated in combination with genome assemblies ... |
publisher |
Dryad |
publishDate |
2023 |
url |
https://dx.doi.org/10.5061/dryad.wh70rxwvb https://datadryad.org/stash/dataset/doi:10.5061/dryad.wh70rxwvb |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Humpback Whale Southern Right Whale |
genre_facet |
Humpback Whale Southern Right Whale |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.wh70rxwvb |
_version_ |
1801376568085315584 |