Diversification patterns in Boeckella ...

• Main Author: Maturana, Claudia
• Format: Dataset
• Language: English
• Published: Dryad 2021
• Subjects: FOS Biological sciences; Antarctica; Australasia; Biogeography; cox1; Gondwana; ITS; South America; 28S rRNA; Boeckella; Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.5061/dryad.w9ghx3fpv; https://datadryad.org/stash/dataset/doi:10.5061/dryad.w9ghx3fpv

Aim: We investigated evolutionary relationships and biogeographical patterns within the genus Boeckella to evaluate: 1) whether its current widespread distribution in the Southern Hemisphere is due to recent long-distance dispersal or long-term diversification; and 2) the age and origin of sub-Antarctic and Antarctic Boeckella species, with particular focus on the most widely distributed species: B. poppei. Location: South America, sub-Antarctic islands, maritime Antarctica, continental Antarctica and Australasia. Methods: To reconstruct phylogenetic patterns of Boeckella, we used molecular sequence data collected from 12 regions, and applied Bayesian and Maximum Likelihood analyses using multiple loci. We also estimated divergence times and reconstructed ancestral ranges using two different models of species evolution. Results: Phylogenetic analyses and divergence time estimates suggested that Boeckella originated on the Gondwanan supercontinent and initially split into two main clades during the late ... : We extracted DNA from entire individuals using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany), with a modified protocol for small amounts of tissue (see Usage notes). Three different loci were amplified including a fragment of the mitochondrial cytochrome c oxidase subunit I (cox1) gene, the nuclear 28S rRNA gene and a segment spanning the Internal Transcribed Spacers 1 and 2 (ITS hereafter). For cox1 2.5mL 10X Buffer (50 mM KCl, 10 mM Tris-HCl, pH 8.0), 0.9 mL of 50 mM MgCl2, 200 mM dNTPs, 1mL of each primer (10 pg/mL), 0.5 mL BSA 10 mg/mL, 1 U Taq polymerase (Invitrogen), 14.5 mL of double-distilled water and 4 mL of DNA. Thermal cycling parameters for cox1 were modified from Scheihing et al., (2009), and included an initial denaturation step at 94ºC for 1 min, followed by 10 cycles at 94°C for 1 min, 40°C for 90 sec and 72°C for 1 min, followed by 30 cycles at 94°C for 1 min, 46°C for 90 sec and 72°C for 90 sec, and a final 10 min extension at 72ºC. For 28S PCR reaction 2.5 mL 10X Buffer, 1 ...