Data for: Northward range extension for Durvillaea poha bull kelp: response to tectonic disturbance? ...

• Main Authors: Vaux, Felix, Craw, Dave, Fraser, Ceridwen I., Waters, Jonathan M.
• Format: Dataset
• Language: English
• Published: Dryad 2021
• Subjects: FOS: Biological sciences; Durvillaea; Durvillaea antarctica; Durvillaea poha; GBS genotyping-by-sequencing; Biogeography; New Zealand; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.5061/dryad.v41ns1rw6; https://datadryad.org/stash/dataset/doi:10.5061/dryad.v41ns1rw6

Data for: Vaux F, Craw D, Fraser CI, Waters JM. (2021). Northward range extension for Durvillaea poha bull kelp: response to tectonic disturbance? Journal of Phycology. https://doi.org/10.1111/jpy.13179 Understanding the forces that shape species distributions is increasingly important in a fast-changing world. Although major disturbance events can adversely affect natural populations, they can also present new opportunities, for example by opening up habitat for colonisation by other lineages. Following extensive geographic sampling, we use genomic data to infer a range extension following disturbance for an ecologically important intertidal macroalgal species. Specifically, we genotyped 288 bull-kelp (Durvillaea) plants from 28 localities across central New Zealand. All specimens from the North Island were expected to be D. antarctica, but unexpectedly 10 samples from four sites were unexpectedly identified as D. poha. Extensive sampling from the northern South Island (105 samples at five locations) ... : DNA was extracted and purified following the same method described in Peters et al. (2020), except that the new Qiagen DNeasy Plant Pro DNA extraction kit replaced the equivalent decommissioned Qiagen PowerPlant Pro kit. Samples were used for genotyping-by-sequencing (GBS; Elshire et al. 2011). 14 new D. poha samples were sequenced across three GBS libraries, which contained a total of 340 Durvillaea southern bull-kelp samples, plus 61 samples belonging to unrelated taxa. The GBS sequencing followed an almost identical method to the previous studies (Parvizi et al. 2020, Peters et al. 2020), with size selections varying between 200 – 500 bp and 200 – 600 bp. stacks 2.53 (Rochette et al. 2019) was used to demultiplex samples into paired forward and reverse reads per individual, call loci, and estimate genotypic variation among samples. ...