Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet
Understanding the role of predators in food webs can be challenging in highly diverse predator/prey systems composed of small cryptic species. DNA based dietary analysis can supplement predator removal experiments and provide high resolution for prey identification. Here we use a metabarcoding appro...
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Format: | Dataset |
Language: | English |
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Dryad
2016
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Online Access: | https://dx.doi.org/10.5061/dryad.v0p71 http://datadryad.org/stash/dataset/doi:10.5061/dryad.v0p71 |
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ftdatacite:10.5061/dryad.v0p71 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Camallanidae Vanikoridae Miraciidae Coelosphaeridae Temora discaudata Eviota Trematoda Actaeinae Autolytinae Menaethius monoceros Copepoda Echinoidea Caranx melampygus Chromis vanderbilti Liocarpilodes integerrimus Trapezia areolata Polychaeta Trapezia rufopunctata Cirratulidae Palaemonella tenuipes Lienardia mighelsi Thalassoma hardwicke Gammaropsis Maxillopoda Lysiosquillidae Harpacticus Cerithium Enterogona Gobiidae Terebellidae Pomachromis fuscidorsalis Tanaidacea Trapezia bidentata Petrolisthes Chlorurus sordidus Syllidae Maera Pandalidae Ampharetidae Palaemonidae Thor Poecilostomatoida Gigartinales Apogon nigrofasciatus Huenia Hapalocarcinus Pagurixus Lithoglyptidae Palaemonella rotumana Mysida Scarus globiceps Galatheidae Palmyria Pilodius Plumularia Demospongiae Agalmatidae Clionaidae Decapterus macarellus Emiliania huxleyi Phylladiorhynchus Dolabrifera dolabrifera Paranamixis fijiensis Gelidiaceae Ophiactis Brachyura Nemichthys Anthuridea Aphelenchoididae Argonautidae Clausocalanus arcuicornis Nephtyidae Dendropoma Eviota disrupta Amblycirrhitus bimacula Amphilochus Santiidae Plectroglyphidodon dickii Chromis margaritifer Titanoderma prototypum Enchelyurus ater Manaethius monoceros Xanthias lamarcki Atergatopsis germaini Caranx sexfasciatus Calcinus morgani Cerithiidae Dascyllus flavicaudus Bemlos waipio Viriola incisa Gymnothorax buroensis Chlorodiella laevissima Corallinaceae Sagittidae Candacia ethiopica Harpacticoida Phylladiorhynchus integrirostris Azadinium poporum Gastropoda Phenacolepadidae Homalopoma maculosa Ctenochaetus striatus Amphipoda Clausocalanus minor Scarus psittacus Alpheidae Sipuncula Caridea Chromis viridis Esola Isopoda Jonesius triunguiculatus Calanoida Ceramiales Cirripectes variolosus Petalifera Didemnidae Perciformes Plectroglyphidodon johnstonianus Ptychoderidae Chlorodiella crispipleopa Corycaeus Siphonostomatoida Pseudocheilinus hexataenia Sebastapistes tinkhami Cuapetes ensifrons Pocillopora Stegastes nigricans Gomphosus varius Pascula muricata Acartia negligens Chaetodon trichrous Zebrasoma scopas Dascyllus aruanus Stomatolina rubra Vitricithna marmorata Oithonidae Perinia tumida Hydrozoa Cyclodius ungulatus Halopteris constricta Hippolytidae Pilodius pugil Arete indicus Polynesoecetes kekeae trophic interactions Raoulserenea ornata Fennera chacei Oncaeidae Trapeziidae Bunodeopsis medusoides Lachnopodus subacutus Cestoda Halichoeres hortulanus Stylocheilus striatus Erythrotrichiaceae Monetaria annulus Sargassaceae Trapezia serenei Ophiocoma pica Decapoda Harpiliopsis beaupresii Drupa ricinus 3 days Notopygos Polynoidae Galathea mauritiana Margaretta Verrucidae Monetaria caputdraconis Alpheus dolerus Haminoea natalensis Paguridae Cheilopogon pitcairnensis Munnidae Pilodius flavus Turbellaria Chlorodiella barbata Clausocalanus furcatus Dendropoma gregaria Malacostraca |
spellingShingle |
Camallanidae Vanikoridae Miraciidae Coelosphaeridae Temora discaudata Eviota Trematoda Actaeinae Autolytinae Menaethius monoceros Copepoda Echinoidea Caranx melampygus Chromis vanderbilti Liocarpilodes integerrimus Trapezia areolata Polychaeta Trapezia rufopunctata Cirratulidae Palaemonella tenuipes Lienardia mighelsi Thalassoma hardwicke Gammaropsis Maxillopoda Lysiosquillidae Harpacticus Cerithium Enterogona Gobiidae Terebellidae Pomachromis fuscidorsalis Tanaidacea Trapezia bidentata Petrolisthes Chlorurus sordidus Syllidae Maera Pandalidae Ampharetidae Palaemonidae Thor Poecilostomatoida Gigartinales Apogon nigrofasciatus Huenia Hapalocarcinus Pagurixus Lithoglyptidae Palaemonella rotumana Mysida Scarus globiceps Galatheidae Palmyria Pilodius Plumularia Demospongiae Agalmatidae Clionaidae Decapterus macarellus Emiliania huxleyi Phylladiorhynchus Dolabrifera dolabrifera Paranamixis fijiensis Gelidiaceae Ophiactis Brachyura Nemichthys Anthuridea Aphelenchoididae Argonautidae Clausocalanus arcuicornis Nephtyidae Dendropoma Eviota disrupta Amblycirrhitus bimacula Amphilochus Santiidae Plectroglyphidodon dickii Chromis margaritifer Titanoderma prototypum Enchelyurus ater Manaethius monoceros Xanthias lamarcki Atergatopsis germaini Caranx sexfasciatus Calcinus morgani Cerithiidae Dascyllus flavicaudus Bemlos waipio Viriola incisa Gymnothorax buroensis Chlorodiella laevissima Corallinaceae Sagittidae Candacia ethiopica Harpacticoida Phylladiorhynchus integrirostris Azadinium poporum Gastropoda Phenacolepadidae Homalopoma maculosa Ctenochaetus striatus Amphipoda Clausocalanus minor Scarus psittacus Alpheidae Sipuncula Caridea Chromis viridis Esola Isopoda Jonesius triunguiculatus Calanoida Ceramiales Cirripectes variolosus Petalifera Didemnidae Perciformes Plectroglyphidodon johnstonianus Ptychoderidae Chlorodiella crispipleopa Corycaeus Siphonostomatoida Pseudocheilinus hexataenia Sebastapistes tinkhami Cuapetes ensifrons Pocillopora Stegastes nigricans Gomphosus varius Pascula muricata Acartia negligens Chaetodon trichrous Zebrasoma scopas Dascyllus aruanus Stomatolina rubra Vitricithna marmorata Oithonidae Perinia tumida Hydrozoa Cyclodius ungulatus Halopteris constricta Hippolytidae Pilodius pugil Arete indicus Polynesoecetes kekeae trophic interactions Raoulserenea ornata Fennera chacei Oncaeidae Trapeziidae Bunodeopsis medusoides Lachnopodus subacutus Cestoda Halichoeres hortulanus Stylocheilus striatus Erythrotrichiaceae Monetaria annulus Sargassaceae Trapezia serenei Ophiocoma pica Decapoda Harpiliopsis beaupresii Drupa ricinus 3 days Notopygos Polynoidae Galathea mauritiana Margaretta Verrucidae Monetaria caputdraconis Alpheus dolerus Haminoea natalensis Paguridae Cheilopogon pitcairnensis Munnidae Pilodius flavus Turbellaria Chlorodiella barbata Clausocalanus furcatus Dendropoma gregaria Malacostraca Leray, Matthieu Meyer, Christopher P. Mills, Suzanne C. Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet |
topic_facet |
Camallanidae Vanikoridae Miraciidae Coelosphaeridae Temora discaudata Eviota Trematoda Actaeinae Autolytinae Menaethius monoceros Copepoda Echinoidea Caranx melampygus Chromis vanderbilti Liocarpilodes integerrimus Trapezia areolata Polychaeta Trapezia rufopunctata Cirratulidae Palaemonella tenuipes Lienardia mighelsi Thalassoma hardwicke Gammaropsis Maxillopoda Lysiosquillidae Harpacticus Cerithium Enterogona Gobiidae Terebellidae Pomachromis fuscidorsalis Tanaidacea Trapezia bidentata Petrolisthes Chlorurus sordidus Syllidae Maera Pandalidae Ampharetidae Palaemonidae Thor Poecilostomatoida Gigartinales Apogon nigrofasciatus Huenia Hapalocarcinus Pagurixus Lithoglyptidae Palaemonella rotumana Mysida Scarus globiceps Galatheidae Palmyria Pilodius Plumularia Demospongiae Agalmatidae Clionaidae Decapterus macarellus Emiliania huxleyi Phylladiorhynchus Dolabrifera dolabrifera Paranamixis fijiensis Gelidiaceae Ophiactis Brachyura Nemichthys Anthuridea Aphelenchoididae Argonautidae Clausocalanus arcuicornis Nephtyidae Dendropoma Eviota disrupta Amblycirrhitus bimacula Amphilochus Santiidae Plectroglyphidodon dickii Chromis margaritifer Titanoderma prototypum Enchelyurus ater Manaethius monoceros Xanthias lamarcki Atergatopsis germaini Caranx sexfasciatus Calcinus morgani Cerithiidae Dascyllus flavicaudus Bemlos waipio Viriola incisa Gymnothorax buroensis Chlorodiella laevissima Corallinaceae Sagittidae Candacia ethiopica Harpacticoida Phylladiorhynchus integrirostris Azadinium poporum Gastropoda Phenacolepadidae Homalopoma maculosa Ctenochaetus striatus Amphipoda Clausocalanus minor Scarus psittacus Alpheidae Sipuncula Caridea Chromis viridis Esola Isopoda Jonesius triunguiculatus Calanoida Ceramiales Cirripectes variolosus Petalifera Didemnidae Perciformes Plectroglyphidodon johnstonianus Ptychoderidae Chlorodiella crispipleopa Corycaeus Siphonostomatoida Pseudocheilinus hexataenia Sebastapistes tinkhami Cuapetes ensifrons Pocillopora Stegastes nigricans Gomphosus varius Pascula muricata Acartia negligens Chaetodon trichrous Zebrasoma scopas Dascyllus aruanus Stomatolina rubra Vitricithna marmorata Oithonidae Perinia tumida Hydrozoa Cyclodius ungulatus Halopteris constricta Hippolytidae Pilodius pugil Arete indicus Polynesoecetes kekeae trophic interactions Raoulserenea ornata Fennera chacei Oncaeidae Trapeziidae Bunodeopsis medusoides Lachnopodus subacutus Cestoda Halichoeres hortulanus Stylocheilus striatus Erythrotrichiaceae Monetaria annulus Sargassaceae Trapezia serenei Ophiocoma pica Decapoda Harpiliopsis beaupresii Drupa ricinus 3 days Notopygos Polynoidae Galathea mauritiana Margaretta Verrucidae Monetaria caputdraconis Alpheus dolerus Haminoea natalensis Paguridae Cheilopogon pitcairnensis Munnidae Pilodius flavus Turbellaria Chlorodiella barbata Clausocalanus furcatus Dendropoma gregaria Malacostraca |
description |
Understanding the role of predators in food webs can be challenging in highly diverse predator/prey systems composed of small cryptic species. DNA based dietary analysis can supplement predator removal experiments and provide high resolution for prey identification. Here we use a metabarcoding approach to provide initial insights into the diet and functional role of coral-dwelling predatory fish feeding on small invertebrates. Fish were collected in Moorea (French Polynesia) where the BIOCODE project has generated DNA barcodes for numerous coral associated invertebrate species. Pyrosequencing data revealed a total of 292 Operational Taxonomic Units (OTU) in the gut contents of the arc-eye hawkfish (Paracirrhites arcatus), the flame hawkfish (Neocirrhites armatus) and the coral croucher (Caracanthus maculatus). One hundred forty-nine (51%) of them had species-level matches in reference libraries (>98% similarity) while 76 additional OTUs (26%) could be identified to higher taxonomic levels. Decapods that have a mutualistic relationship with Pocillopora and are typically dominant among coral branches, represent a minor contribution of the predators’ diets. Instead, predators mainly consumed transient species including pelagic taxa such as copepods, chaetognaths and siphonophores suggesting non random feeding behavior. We also identified prey species known to have direct negative interactions with stony corals, such as Hapalocarcinus sp, a gall crab considered a coral parasite, as well as species of vermetid snails known for their deleterious effects on coral growth. Pocillopora DNA accounted for 20.8% and 20.1% of total number of sequences in the guts of the flame hawkfish and coral croucher but it was not detected in the guts of the arc-eye hawkfish. Comparison of diets among the three fishes demonstrates remarkable partitioning with nearly 80% of prey items consumed by only one predator. Overall, the taxonomic resolution provided by the metabarcoding approach highlights a highly complex interaction web and demonstrates that levels of trophic partitioning among coral reef fishes have likely been underestimated. Therefore, we strongly encourage further empirical approaches to dietary studies prior to making assumptions of trophic equivalency in food web reconstruction. : 454 reads - fasta formatThis files contains all unique reads that passed the initial denoising step as specified in the manuscript.Poc_associates.shhh.fasta454 name fileit contains the name of all reads of the denoised data file that are identical to the representative reads shown in the fasta file. The first column contains the representative sequence's name. The second column contains the names of its representatives separated by commas.Poc_associates.shhh.names454 group fileIt contains the name of the sample each 454 read belongs to. FL: Neocirrhites armatus; AEH: Paracirrhites arcatus; CC: Caracanthus maculatus.Poc_associates.shhh.groups |
format |
Dataset |
author |
Leray, Matthieu Meyer, Christopher P. Mills, Suzanne C. |
author_facet |
Leray, Matthieu Meyer, Christopher P. Mills, Suzanne C. |
author_sort |
Leray, Matthieu |
title |
Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet |
title_short |
Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet |
title_full |
Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet |
title_fullStr |
Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet |
title_full_unstemmed |
Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet |
title_sort |
data from: metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet |
publisher |
Dryad |
publishDate |
2016 |
url |
https://dx.doi.org/10.5061/dryad.v0p71 http://datadryad.org/stash/dataset/doi:10.5061/dryad.v0p71 |
genre |
Copepods Harpacticus |
genre_facet |
Copepods Harpacticus |
op_relation |
https://dx.doi.org/10.7717/peerj.1047 |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_rightsnorm |
CC0 |
op_doi |
https://doi.org/10.5061/dryad.v0p71 https://doi.org/10.7717/peerj.1047 |
_version_ |
1766243376127016960 |
spelling |
ftdatacite:10.5061/dryad.v0p71 2023-05-15T18:49:46+02:00 Data from: Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet Leray, Matthieu Meyer, Christopher P. Mills, Suzanne C. 2016 https://dx.doi.org/10.5061/dryad.v0p71 http://datadryad.org/stash/dataset/doi:10.5061/dryad.v0p71 en eng Dryad https://dx.doi.org/10.7717/peerj.1047 Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 CC0 Camallanidae Vanikoridae Miraciidae Coelosphaeridae Temora discaudata Eviota Trematoda Actaeinae Autolytinae Menaethius monoceros Copepoda Echinoidea Caranx melampygus Chromis vanderbilti Liocarpilodes integerrimus Trapezia areolata Polychaeta Trapezia rufopunctata Cirratulidae Palaemonella tenuipes Lienardia mighelsi Thalassoma hardwicke Gammaropsis Maxillopoda Lysiosquillidae Harpacticus Cerithium Enterogona Gobiidae Terebellidae Pomachromis fuscidorsalis Tanaidacea Trapezia bidentata Petrolisthes Chlorurus sordidus Syllidae Maera Pandalidae Ampharetidae Palaemonidae Thor Poecilostomatoida Gigartinales Apogon nigrofasciatus Huenia Hapalocarcinus Pagurixus Lithoglyptidae Palaemonella rotumana Mysida Scarus globiceps Galatheidae Palmyria Pilodius Plumularia Demospongiae Agalmatidae Clionaidae Decapterus macarellus Emiliania huxleyi Phylladiorhynchus Dolabrifera dolabrifera Paranamixis fijiensis Gelidiaceae Ophiactis Brachyura Nemichthys Anthuridea Aphelenchoididae Argonautidae Clausocalanus arcuicornis Nephtyidae Dendropoma Eviota disrupta Amblycirrhitus bimacula Amphilochus Santiidae Plectroglyphidodon dickii Chromis margaritifer Titanoderma prototypum Enchelyurus ater Manaethius monoceros Xanthias lamarcki Atergatopsis germaini Caranx sexfasciatus Calcinus morgani Cerithiidae Dascyllus flavicaudus Bemlos waipio Viriola incisa Gymnothorax buroensis Chlorodiella laevissima Corallinaceae Sagittidae Candacia ethiopica Harpacticoida Phylladiorhynchus integrirostris Azadinium poporum Gastropoda Phenacolepadidae Homalopoma maculosa Ctenochaetus striatus Amphipoda Clausocalanus minor Scarus psittacus Alpheidae Sipuncula Caridea Chromis viridis Esola Isopoda Jonesius triunguiculatus Calanoida Ceramiales Cirripectes variolosus Petalifera Didemnidae Perciformes Plectroglyphidodon johnstonianus Ptychoderidae Chlorodiella crispipleopa Corycaeus Siphonostomatoida Pseudocheilinus hexataenia Sebastapistes tinkhami Cuapetes ensifrons Pocillopora Stegastes nigricans Gomphosus varius Pascula muricata Acartia negligens Chaetodon trichrous Zebrasoma scopas Dascyllus aruanus Stomatolina rubra Vitricithna marmorata Oithonidae Perinia tumida Hydrozoa Cyclodius ungulatus Halopteris constricta Hippolytidae Pilodius pugil Arete indicus Polynesoecetes kekeae trophic interactions Raoulserenea ornata Fennera chacei Oncaeidae Trapeziidae Bunodeopsis medusoides Lachnopodus subacutus Cestoda Halichoeres hortulanus Stylocheilus striatus Erythrotrichiaceae Monetaria annulus Sargassaceae Trapezia serenei Ophiocoma pica Decapoda Harpiliopsis beaupresii Drupa ricinus 3 days Notopygos Polynoidae Galathea mauritiana Margaretta Verrucidae Monetaria caputdraconis Alpheus dolerus Haminoea natalensis Paguridae Cheilopogon pitcairnensis Munnidae Pilodius flavus Turbellaria Chlorodiella barbata Clausocalanus furcatus Dendropoma gregaria Malacostraca dataset Dataset 2016 ftdatacite https://doi.org/10.5061/dryad.v0p71 https://doi.org/10.7717/peerj.1047 2022-02-08T12:53:43Z Understanding the role of predators in food webs can be challenging in highly diverse predator/prey systems composed of small cryptic species. DNA based dietary analysis can supplement predator removal experiments and provide high resolution for prey identification. Here we use a metabarcoding approach to provide initial insights into the diet and functional role of coral-dwelling predatory fish feeding on small invertebrates. Fish were collected in Moorea (French Polynesia) where the BIOCODE project has generated DNA barcodes for numerous coral associated invertebrate species. Pyrosequencing data revealed a total of 292 Operational Taxonomic Units (OTU) in the gut contents of the arc-eye hawkfish (Paracirrhites arcatus), the flame hawkfish (Neocirrhites armatus) and the coral croucher (Caracanthus maculatus). One hundred forty-nine (51%) of them had species-level matches in reference libraries (>98% similarity) while 76 additional OTUs (26%) could be identified to higher taxonomic levels. Decapods that have a mutualistic relationship with Pocillopora and are typically dominant among coral branches, represent a minor contribution of the predators’ diets. Instead, predators mainly consumed transient species including pelagic taxa such as copepods, chaetognaths and siphonophores suggesting non random feeding behavior. We also identified prey species known to have direct negative interactions with stony corals, such as Hapalocarcinus sp, a gall crab considered a coral parasite, as well as species of vermetid snails known for their deleterious effects on coral growth. Pocillopora DNA accounted for 20.8% and 20.1% of total number of sequences in the guts of the flame hawkfish and coral croucher but it was not detected in the guts of the arc-eye hawkfish. Comparison of diets among the three fishes demonstrates remarkable partitioning with nearly 80% of prey items consumed by only one predator. Overall, the taxonomic resolution provided by the metabarcoding approach highlights a highly complex interaction web and demonstrates that levels of trophic partitioning among coral reef fishes have likely been underestimated. Therefore, we strongly encourage further empirical approaches to dietary studies prior to making assumptions of trophic equivalency in food web reconstruction. : 454 reads - fasta formatThis files contains all unique reads that passed the initial denoising step as specified in the manuscript.Poc_associates.shhh.fasta454 name fileit contains the name of all reads of the denoised data file that are identical to the representative reads shown in the fasta file. The first column contains the representative sequence's name. The second column contains the names of its representatives separated by commas.Poc_associates.shhh.names454 group fileIt contains the name of the sample each 454 read belongs to. FL: Neocirrhites armatus; AEH: Paracirrhites arcatus; CC: Caracanthus maculatus.Poc_associates.shhh.groups Dataset Copepods Harpacticus DataCite Metadata Store (German National Library of Science and Technology) |