Data from: A molecular gut content study of Themisto abyssorum (Amphipoda) from Arctic hydrothermal vent and cold seep systems ...

• Main Authors: Olsen, Bernt Rydland, Troedsson, Christofer, Hadziavdic, Kenan, Rapp, Hans Tore, Pedersen, Rolf B.
• Format: Dataset
• Language: English
• Published: Dryad 2013
• Subjects: 18s rDNA; DHPLC; Hyperiidae; Themisto abyssorum; Copepoda; Universal Primers; Trophic interactions; Amphipoda; Arctic; Jan Mayen; Nordic Seas; Themisto
• Online Access: https://dx.doi.org/10.5061/dryad.tm1k0; https://datadryad.org/stash/dataset/doi:10.5061/dryad.tm1k0

The use of DNA as a marker for prey inside the gut of predators has been instrumental in further understanding of known and unknown interactions. Molecular approaches are in particular useful in unavailable environments like the deep-sea. Trophic interactions in the deep-sea are difficult to observe in situ, correct deep-sea experimental laboratory conditions are difficult to obtain, animals rarely survive the sampling, or the study organisms feed during the sampling due to long hauls. Preliminary studies of vent and seep systems in the Nordic Seas have identified the temperate-cold water pelagic amphipod Themisto abyssorum as a potentially important predator these chemosynthetic habitats. However, the prey of this deep-sea predator is poorly known, and we applied Denaturing High Performance Liquid Chromatography (DHPLC) to investigate the predator - prey interactions of T. abyssorum in deep-water vent and seep systems. Two deep-water hydrothermally active localities (The Jan Mayen and Loki's Castle vent ... : OTU_best_rep_97_similarityBest representative sequences from each OTU based on 97% similarity.OTU_best_rep_98_similarityBest representative sequences from each OTU based on 98% similarity.OTU_best_rep_99_similarityBest representative sequences from each OTU based on 99% similarity.OTU_matrix_97_similarityA presence absence matrix for each OTU and specimen using 97% similarity to assign OTUsOTU_matrix_98_similarityA presence absence matrix for each OTU and specimen using 98% similarity to assign OTUs. See read me file in 'OTU_matrix_97_similarity.tab'OTU_matrix_99_similarityA presence absence matrix for each OTU and specimen using 99% similarity to assign OTUs. See read me file in 'OTU_matrix_97_similarity.tab'all_sequences_tab_delimitedAll sequences used in this studyTaxonomy_WORMSThe basis for the annotation of all OTUs in the study. Must be used together with the OTU matrixes to be able to get any geographical data from it. ...