Pansu&al_BioLett_gh_unfiltered_dataset.txt
This table contains pre-filtered sequencing data (i.e. usable merged reads assigned to their original sample) for fungal metabarcode. Amplicons were amplified using the univerdal primers 'g' (5'-GGGCAATCCTGAGCCAA-3') and 'h' (5'-CCATTGAGTCTCTGCACCTATC-3') prim...
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Dryad Digital Repository
2015
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Online Access: | https://dx.doi.org/10.5061/dryad.t8534/7 http://datadryad.org/resource/doi:10.5061/dryad.t8534/7 |
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ftdatacite:10.5061/dryad.t8534/7 2023-05-15T13:53:29+02:00 Pansu&al_BioLett_gh_unfiltered_dataset.txt Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe 2015 https://dx.doi.org/10.5061/dryad.t8534/7 http://datadryad.org/resource/doi:10.5061/dryad.t8534/7 unknown Dryad Digital Repository https://dx.doi.org/10.5061/dryad.t8534 http://creativecommons.org/publicdomain/zero/1.0 CC0 Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Embryophyta dataset Dataset DataFile 2015 ftdatacite https://doi.org/10.5061/dryad.t8534/7 https://doi.org/10.5061/dryad.t8534 2021-11-05T12:55:41Z This table contains pre-filtered sequencing data (i.e. usable merged reads assigned to their original sample) for fungal metabarcode. Amplicons were amplified using the univerdal primers 'g' (5'-GGGCAATCCTGAGCCAA-3') and 'h' (5'-CCATTGAGTCTCTGCACCTATC-3') primers (Taberlet et al. 2007). Sequences were produced by a 2 x 100 bp paired-end sequencing on Illumina HiSeq 2500 platform. First processing steps were performed using the OBITOOLS software (http://metabarcoding.org/obitools) as follows: (i) Direct and reverse reads corresponding to the same sequence were aligned and merged thanks to the IlluminaPairEnd program. Only merged sequences with a high alignment quality score were retained (>=40). (ii) Each merged sequence was assigned to its original sample using the tags information previously added to primers thanks to the ngsfilter program. For this step, only sequences containing both primers (with a maximum of 3 mismatches per primer) and exact tag sequences were selected. (iii) To reduce the file size, strictly identical sequences were merged together while keeping information about the origin of sequences. (iv) Sequences containing ambiguous nucleotides or shorter than 7 bp were discarded. (v) Pure singleton (sequences with 1 read among the whole dataset) were removed. Dataset Antarc* Antarctic Kerguelen Islands DataCite Metadata Store (German National Library of Science and Technology) Antarctic Kerguelen Kerguelen Islands |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Embryophyta |
spellingShingle |
Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Embryophyta Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe Pansu&al_BioLett_gh_unfiltered_dataset.txt |
topic_facet |
Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Embryophyta |
description |
This table contains pre-filtered sequencing data (i.e. usable merged reads assigned to their original sample) for fungal metabarcode. Amplicons were amplified using the univerdal primers 'g' (5'-GGGCAATCCTGAGCCAA-3') and 'h' (5'-CCATTGAGTCTCTGCACCTATC-3') primers (Taberlet et al. 2007). Sequences were produced by a 2 x 100 bp paired-end sequencing on Illumina HiSeq 2500 platform. First processing steps were performed using the OBITOOLS software (http://metabarcoding.org/obitools) as follows: (i) Direct and reverse reads corresponding to the same sequence were aligned and merged thanks to the IlluminaPairEnd program. Only merged sequences with a high alignment quality score were retained (>=40). (ii) Each merged sequence was assigned to its original sample using the tags information previously added to primers thanks to the ngsfilter program. For this step, only sequences containing both primers (with a maximum of 3 mismatches per primer) and exact tag sequences were selected. (iii) To reduce the file size, strictly identical sequences were merged together while keeping information about the origin of sequences. (iv) Sequences containing ambiguous nucleotides or shorter than 7 bp were discarded. (v) Pure singleton (sequences with 1 read among the whole dataset) were removed. |
format |
Dataset |
author |
Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe |
author_facet |
Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe |
author_sort |
Pansu, Johan |
title |
Pansu&al_BioLett_gh_unfiltered_dataset.txt |
title_short |
Pansu&al_BioLett_gh_unfiltered_dataset.txt |
title_full |
Pansu&al_BioLett_gh_unfiltered_dataset.txt |
title_fullStr |
Pansu&al_BioLett_gh_unfiltered_dataset.txt |
title_full_unstemmed |
Pansu&al_BioLett_gh_unfiltered_dataset.txt |
title_sort |
pansu&al_biolett_gh_unfiltered_dataset.txt |
publisher |
Dryad Digital Repository |
publishDate |
2015 |
url |
https://dx.doi.org/10.5061/dryad.t8534/7 http://datadryad.org/resource/doi:10.5061/dryad.t8534/7 |
geographic |
Antarctic Kerguelen Kerguelen Islands |
geographic_facet |
Antarctic Kerguelen Kerguelen Islands |
genre |
Antarc* Antarctic Kerguelen Islands |
genre_facet |
Antarc* Antarctic Kerguelen Islands |
op_relation |
https://dx.doi.org/10.5061/dryad.t8534 |
op_rights |
http://creativecommons.org/publicdomain/zero/1.0 |
op_rightsnorm |
CC0 |
op_doi |
https://doi.org/10.5061/dryad.t8534/7 https://doi.org/10.5061/dryad.t8534 |
_version_ |
1766258644001751040 |