Filtered sequencing data (MOTUs) for fungal metabarcode

• Main Authors: Pansu, Johan, Winkworth, Richard C., Hennion, Françoise, Gielly, Ludovic, Taberlet, Pierre, Choler, Philippe
• Format: Dataset
• Language: unknown
• Published: Dryad Digital Repository 2015
• Subjects: Environmental DNA; Invasive species; Metabarcoding; Soil communities; Kerguelen Islands; Sub-Antarctic Islands; Fungi; Antarctic; Kerguelen; Antarc*
• Online Access: https://dx.doi.org/10.5061/dryad.t8534/4; http://datadryad.org/resource/doi:10.5061/dryad.t8534/4

This table contains filtered MOTUs for the fungal metabarcode. Amplicons were amplified using ITS5 : 5'-GGAAGTAAAAGTCGTAACAAGG-3' (White et al. 1990) and 5.8S_fungi : 5'- CAAGAGATCCGTTGTTGAAAGTT-3' primers (Epp et al. 2012). Sequences were obtained by a 2 x 250 bp paired-end sequencing on Illumina MiSeq platform. Data filtering and MOTUs clustering were performed according to the procedure described in the supplementary materials of Pansu et al. (2015): 'Long-lasting modification of soil fungal diversity associated with the introduction of rabbits to a remote sub-Antarctic archipelago'.