Unfiltered sequencing data for fungal metabarcode (fasta format)
This fasta file contains unfiltered sequencing data (i.e. merged reads assigned to their original sample) for fungal metabarcode. Amplicons were amplified using the primers ITS5 : 5'-GGAAGTAAAAGTCGTAACAAGG-3' (White et al. 1990) and 5.8S_fungi : 5'- CAAGAGATCCGTTGTTGAAAGTT-3' pri...
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Online Access: | https://dx.doi.org/10.5061/dryad.t8534/3 http://datadryad.org/resource/doi:10.5061/dryad.t8534/3 |
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ftdatacite:10.5061/dryad.t8534/3 2023-05-15T13:53:29+02:00 Unfiltered sequencing data for fungal metabarcode (fasta format) Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe 2015 https://dx.doi.org/10.5061/dryad.t8534/3 http://datadryad.org/resource/doi:10.5061/dryad.t8534/3 unknown Dryad Digital Repository https://dx.doi.org/10.5061/dryad.t8534 http://creativecommons.org/publicdomain/zero/1.0 CC0 Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Fungi dataset Dataset DataFile 2015 ftdatacite https://doi.org/10.5061/dryad.t8534/3 https://doi.org/10.5061/dryad.t8534 2021-11-05T12:55:41Z This fasta file contains unfiltered sequencing data (i.e. merged reads assigned to their original sample) for fungal metabarcode. Amplicons were amplified using the primers ITS5 : 5'-GGAAGTAAAAGTCGTAACAAGG-3' (White et al. 1990) and 5.8S_fungi : 5'- CAAGAGATCCGTTGTTGAAAGTT-3' primers (Epp et al. 2012). Sequences were produced by a 2 x 250 bp paired-end sequencing on Illumina MiSeq platform. First processing steps were performed using the OBITOOLS software (http://metabarcoding.org/obitools) as follows: (i) Direct and reverse reads corresponding to the same sequence were aligned and merged thanks to the IlluminaPairEnd program. Only merged sequences with a high alignment quality score were retained (>=40) (ii) Each merged sequence was assigned to its original sample using the tags information previously added to primers thanks to the ngsfilter program. For this step, only sequences containing both primers (with a maximum of 3 mismatches per primer) and exact tag sequences were selected. (iii) To reduce the file size, strictly identical sequences were merged together while keeping information about the origin of sequences. Dataset Antarc* Antarctic Kerguelen Islands DataCite Metadata Store (German National Library of Science and Technology) Antarctic Kerguelen Kerguelen Islands |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Fungi |
spellingShingle |
Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Fungi Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe Unfiltered sequencing data for fungal metabarcode (fasta format) |
topic_facet |
Environmental DNA Invasive species Metabarcoding Soil communities Kerguelen Islands Sub-Antarctic Islands Fungi |
description |
This fasta file contains unfiltered sequencing data (i.e. merged reads assigned to their original sample) for fungal metabarcode. Amplicons were amplified using the primers ITS5 : 5'-GGAAGTAAAAGTCGTAACAAGG-3' (White et al. 1990) and 5.8S_fungi : 5'- CAAGAGATCCGTTGTTGAAAGTT-3' primers (Epp et al. 2012). Sequences were produced by a 2 x 250 bp paired-end sequencing on Illumina MiSeq platform. First processing steps were performed using the OBITOOLS software (http://metabarcoding.org/obitools) as follows: (i) Direct and reverse reads corresponding to the same sequence were aligned and merged thanks to the IlluminaPairEnd program. Only merged sequences with a high alignment quality score were retained (>=40) (ii) Each merged sequence was assigned to its original sample using the tags information previously added to primers thanks to the ngsfilter program. For this step, only sequences containing both primers (with a maximum of 3 mismatches per primer) and exact tag sequences were selected. (iii) To reduce the file size, strictly identical sequences were merged together while keeping information about the origin of sequences. |
format |
Dataset |
author |
Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe |
author_facet |
Pansu, Johan Winkworth, Richard C. Hennion, Françoise Gielly, Ludovic Taberlet, Pierre Choler, Philippe |
author_sort |
Pansu, Johan |
title |
Unfiltered sequencing data for fungal metabarcode (fasta format) |
title_short |
Unfiltered sequencing data for fungal metabarcode (fasta format) |
title_full |
Unfiltered sequencing data for fungal metabarcode (fasta format) |
title_fullStr |
Unfiltered sequencing data for fungal metabarcode (fasta format) |
title_full_unstemmed |
Unfiltered sequencing data for fungal metabarcode (fasta format) |
title_sort |
unfiltered sequencing data for fungal metabarcode (fasta format) |
publisher |
Dryad Digital Repository |
publishDate |
2015 |
url |
https://dx.doi.org/10.5061/dryad.t8534/3 http://datadryad.org/resource/doi:10.5061/dryad.t8534/3 |
geographic |
Antarctic Kerguelen Kerguelen Islands |
geographic_facet |
Antarctic Kerguelen Kerguelen Islands |
genre |
Antarc* Antarctic Kerguelen Islands |
genre_facet |
Antarc* Antarctic Kerguelen Islands |
op_relation |
https://dx.doi.org/10.5061/dryad.t8534 |
op_rights |
http://creativecommons.org/publicdomain/zero/1.0 |
op_rightsnorm |
CC0 |
op_doi |
https://doi.org/10.5061/dryad.t8534/3 https://doi.org/10.5061/dryad.t8534 |
_version_ |
1766258643220561920 |