Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ...
The study of carnivores' diet is a key component to enhance knowledge on the ecology of predators and their effect on prey populations. Although molecular approaches to detect prey DNA in carnivore scats are improving, the validation of their accuracy, a prerequisite for reliable applications w...
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Online Access: | https://dx.doi.org/10.5061/dryad.t1g1jwt70 https://datadryad.org/stash/dataset/doi:10.5061/dryad.t1g1jwt70 |
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ftdatacite:10.5061/dryad.t1g1jwt70 2024-02-04T09:59:28+01:00 Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ... Di Bernardi, Cecilia Wikenros, Camilla Ciucci, Paolo Boitani, Luigi Sand, Håkan Åkesson, Mikael 2023 https://dx.doi.org/10.5061/dryad.t1g1jwt70 https://datadryad.org/stash/dataset/doi:10.5061/dryad.t1g1jwt70 en eng Dryad Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 FOS Biological sciences Canis lupus Diet Analysis DNA False negatives feeding experiments sensitivity Dataset dataset 2023 ftdatacite https://doi.org/10.5061/dryad.t1g1jwt70 2024-01-05T04:51:50Z The study of carnivores' diet is a key component to enhance knowledge on the ecology of predators and their effect on prey populations. Although molecular approaches to detect prey DNA in carnivore scats are improving, the validation of their accuracy, a prerequisite for reliable applications within ecological frameworks, is still lagging behind the methodological advances. Indeed, variation in detection probability among prey species can occur, representing a potentially insidious source of bias in food-habit studies of carnivores. Calibration of DNA-based methods involves the optimization of specificity and sensitivity and, whereas priority is usually given to the former to avoid false positives, sensitivity is rarely investigated so that false negatives may be largely overlooked. We conducted feeding trials with captive wolves (Canis lupus) to validate a nanofluidic array technology recently developed for detection of multiple prey species in scats. Using 371 scat samples from 12 wolves fed with a ... Dataset Canis lupus DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
FOS Biological sciences Canis lupus Diet Analysis DNA False negatives feeding experiments sensitivity |
spellingShingle |
FOS Biological sciences Canis lupus Diet Analysis DNA False negatives feeding experiments sensitivity Di Bernardi, Cecilia Wikenros, Camilla Ciucci, Paolo Boitani, Luigi Sand, Håkan Åkesson, Mikael Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ... |
topic_facet |
FOS Biological sciences Canis lupus Diet Analysis DNA False negatives feeding experiments sensitivity |
description |
The study of carnivores' diet is a key component to enhance knowledge on the ecology of predators and their effect on prey populations. Although molecular approaches to detect prey DNA in carnivore scats are improving, the validation of their accuracy, a prerequisite for reliable applications within ecological frameworks, is still lagging behind the methodological advances. Indeed, variation in detection probability among prey species can occur, representing a potentially insidious source of bias in food-habit studies of carnivores. Calibration of DNA-based methods involves the optimization of specificity and sensitivity and, whereas priority is usually given to the former to avoid false positives, sensitivity is rarely investigated so that false negatives may be largely overlooked. We conducted feeding trials with captive wolves (Canis lupus) to validate a nanofluidic array technology recently developed for detection of multiple prey species in scats. Using 371 scat samples from 12 wolves fed with a ... |
format |
Dataset |
author |
Di Bernardi, Cecilia Wikenros, Camilla Ciucci, Paolo Boitani, Luigi Sand, Håkan Åkesson, Mikael |
author_facet |
Di Bernardi, Cecilia Wikenros, Camilla Ciucci, Paolo Boitani, Luigi Sand, Håkan Åkesson, Mikael |
author_sort |
Di Bernardi, Cecilia |
title |
Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ... |
title_short |
Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ... |
title_full |
Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ... |
title_fullStr |
Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ... |
title_full_unstemmed |
Experimental feeding validates nanofluidic array technology for DNA detection of ungulate prey in wolf scats ... |
title_sort |
experimental feeding validates nanofluidic array technology for dna detection of ungulate prey in wolf scats ... |
publisher |
Dryad |
publishDate |
2023 |
url |
https://dx.doi.org/10.5061/dryad.t1g1jwt70 https://datadryad.org/stash/dataset/doi:10.5061/dryad.t1g1jwt70 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.t1g1jwt70 |
_version_ |
1789964279265361920 |