Data from: Clones or clans: the genetic structure of a deep-sea sponge, Aphrocallistes vastus, in unique sponge reefs of British Columbia, Canada ...

• Main Authors: Brown, Rachel R., Davis, Corey S., Leys, Sally P.
• Format: Dataset
• Language: English
• Published: Dryad 2017
• Subjects: Aphrocallistes vastus; glass sponge reefs; Heterochone calyx; Next Generation Sequencing; Deep-sea; genomic duplication; Single nucleotide polymorphisms; British Columbia; Canada; Sog; Alaska; Glass sponges
• Online Access: https://dx.doi.org/10.5061/dryad.sc0nf; https://datadryad.org/stash/dataset/doi:10.5061/dryad.sc0nf

Understanding patterns of reproduction, dispersal and recruitment in deep-sea communities is increasingly important with the need to manage resource extraction and conserve species diversity. Glass sponges are usually found in deep water (>1000 m) worldwide but form kilometre-long reefs on the continental shelf of British Columbia and Alaska that are under threat from trawling and resource exploration. Due to their deep-water habitat, larvae have not yet been found and the level of genetic connectivity between reefs and nonreef communities is unknown. The genetic structure of Aphrocallistes vastus, the primary reef-building species in the Strait of Georgia (SoG) British Columbia, was studied using single nucleotide polymorphisms (SNPs). Pairwise comparisons of multilocus genotypes were used to assess whether sexual reproduction is common. Structure was examined 1) between individuals in reefs, 2) between reefs and 3) between sites in and outside the SoG. Sixty-seven SNPs were genotyped in 91 samples from ... : Sample_informationAphrocallistes vastus sample metadata including sequencing barcode identifier, full sample name, collection year, sample site, GPS coordinates, depth, and heading of the remote operated vehicle (ROV) during collection. Samples correspond to sequence data and are organized by sequencing barcode. This list include controls (samples sequenced twice as well as one Heterochone calyx control) and samples with poor sequence data which did not contribute to the analyses.Genotypes_ID_dataset_91samples_76lociAphrocallistes vastus single nucleotide polymorphism (SNP) genotypes for full sample set (all samples with adequate sequencing coverage/quality except for interspecies control) including sequencing controls. This dataset was used to examine clonality (identity) across samples. Samples are identified by sequencing barcode with sample site identifiers in the second column. Data are organized into two columns per locus with GenAlEx format (A=1,C=2,G=3,T=4, ...