A single genomic region involving a putative chromosome rearrangement in flat oyster (Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae ...

• Main Authors: Martínez Portela, Paulino, Sambade, Inés, Casanova, Adrian, Blanco, Andrés, Gundappa, Manu, Bean, Tim, Macqueen, Daniel L., Houston, Ross D., Villalba, Antonio, Kamermans, Pauline, Vera, Manuel
• Format: Dataset
• Language: English
• Published: Dryad 2022
• Subjects: Ostrea edulis; bonamiosis; SNP-chip; disease resilience; chromosome rearrangement; FOS Agriculture, forestry, and fisheries; Northeast Atlantic
• Online Access: https://dx.doi.org/10.5061/dryad.s1rn8pkbp; https://datadryad.org/stash/dataset/doi:10.5061/dryad.s1rn8pkbp

European flat oyster (Ostrea edulis) is an ecologically and economically important marine bivalve, that has been severely affected by the intracellular parasite Bonamia ostreae. In this study, a flat oyster SNP array (~14,000 SNPs) was used to validate previously reported outlier loci for divergent selection associated with B. ostreae exposure in the Northeast Atlantic Area. A total of 134 wild and hatchery individuals from the North Sea, collected in naïve (NV) and long-term affected (LTA) areas, were analysed. Genetic diversity and differentiation were related to the sampling origin (wild vs hatchery) when using neutral markers, and to bonamiosis status (NV vs LTA) when using outlier loci for divergent selection. Two genetic clusters appeared intermingled in all sampling locations when using outlier loci and their frequency was associated with their bonamiosis status. When both clusters were compared, outlier datasets showed high genetic divergence (FST > 0.25) unlike neutral loci (FST not ≠ 0). ... : Sampling The analysis was performed on 134 flat oyster individuals collected in 2019 from locations with different bonamiosis prevalence: i) three oyster beds from the North Sea (OS, GBR, NO); ii) two hatchery batches (OSH, WZH) from the same area (FAO subarea 27.4 https://www.fao.org/fishery/en/area/27/en; Fig. 1) (Table 1). Hatchery spat was produced from wild individuals of the same area that were conditioned in the hatchery until spawning. Hatchery samples were included in the study considering their interest in restocking bonamiosis-affected areas by using Bonamia-resilient strains from marker-assisted selection programs. These samples provided additional information for the association analysis and validation of markers, and specifically in one case, the Wadden Sea, the only representative sample was from the hatchery. Furthermore, hatchery data allowed an estimation of the contribution of parents for checking the reproduction protocols in batches at hatcheries to maintain genetic diversity in future ...