Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments ...

• Main Authors: Lindensmith, Christian A., Rider, Stephanie, Bedrossian, Manuel, Wallace, J. Kent, Serabyn, Eugene, Showalter, Gordon M., Deming, Jody W., Nadeau, Jay L.
• Format: Dataset
• Language: English
• Published: Dryad 2017
• Subjects: Astrobiology; Sea ice; motility; marine microorganisms; Holocene; Greenland; Kobbefjord; Nuuk
• Online Access: https://dx.doi.org/10.5061/dryad.rc63v; https://datadryad.org/stash/dataset/doi:10.5061/dryad.rc63v

Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to liquid water below in order to detect life. We have developed a submersible digital holographic microscope (DHM) that is capable of resolving individual bacterial cells, and demonstrated its utility for immediately imaging samples taken directly from sea ice at several locations near Nuuk, Greenland. In all samples, the appearance and motility of eukaryotes were conclusive signs of life. The appearance of prokaryotic cells alone was not sufficient to confirm life, but when prokaryotic motility occurred, it was rapid and conclusive. Warming the samples to above-freezing temperatures or supplementing with serine increased the number of motile cells and the speed of motility; supplementing with serine also stimulated ... : Chemotaxis Middle ChamberHolograms of Malene Bay brine sample exposed to a bottom-to-top serine gradient (data file 2015.03.30 06-28) Data for Fig. 9 B, C in paper and Video S9.Holograms.zipChemotaxis Side ChamberBrine sample exposed to a right-to-left serine gradient. Data file 2015.03.30 06-14. Data for Fig. 9 A in paper.201503300614.zipSeawater at +4CMalene Bay seawater kept overnight at +4 C with the addition of 1/2 strength 2216 marine medium. File 2015.03.30 05-58. Data for Figures 7 and 8E in paper and Videos 4 and 7.201503300558.zipBrine at +4 CMalene Bay brine kept overnight at +4 C with the addition of 1/2 strength 2216 marine medium. File 2015.03.30 05-28. Data for Figures 8F in paper and Video 8.Archive.zipBrine at -4CMalene Bay brine kept overnight at -4 C with no additions. File 2015.03.28 08-11. Data for Figure 8D in paper and Video 6.Archive.zipBrine in situBrine in situ at Kobbefjord. No additives or treatments. Data file 2015.03.26 11-10. Data for Video 3.Archive.zipBrine in ...