Population structure in landrace barley (Hordeum vulgare L.) during the late 19th century crop failures in Fennoscandia ...

• Main Authors: Hagenblad, Jenny, Forsberg, Nils E G, Leino, Matti W
• Format: Dataset
• Language: English
• Published: Dryad 2019
• Subjects: Fennoscandia; SNP genotype data; 19th century; Golden Gate; Norway
• Online Access: https://dx.doi.org/10.5061/dryad.qv9s4mw9b; https://datadryad.org/stash/dataset/doi:10.5061/dryad.qv9s4mw9b

Agricultural disasters and the subsequent need for supply of relief seed can be expected to influence the genetic composition of crop plant populations. The consequences of disasters and seed relief have, however, rarely been studied since specimens sampled before the events are seldomly available. A series of crop failures struck northern Fennoscandia (Norway, Sweden and Finland) during the second half of the 19th century. In order to assess population genetic dynamics of landrace barley (Hordeum vulgare), and consequences of crop failure and possible seed relief during this time period, we genotyped seeds from 16 historical accessions originating from two time periods spanning the period of repeated crop failure. Reliable identification of genetic structuring is highly dependent on sampling regimes and detecting fine-scale geographic or temporal differentiation requires large sample sizes. The robustness of the results under different sampling regimes was evaluated by analyzing subsets of the data and an ... : Individual 19th century seeds were genotyped using an Illumina Golden Gate assay for the C-384 barley SNP set detailed by Moragues et al. (2010). Quality control based on CG10 scores led to the exclusion of 26 low performance samples, including all nine negative controls. Samples with more than 25 % missing data (39 samples), markers with more than 20 % missing data (92 SNPs) and monomorphic SNPs (140 SNPs) were also excluded, in that order. ...