Data from: Distribution and biogeography of Sanguina snow algae: fine-scale sequence analyses reveal previously unknown population structure ...

• Main Author: Brown, Shawn
• Format: Dataset
• Language: English
• Published: Dryad 2020
• Subjects: Snow Algae; Sanguina; MED Node; Argentina; Norway; Svalbard; Antarc*; Antarctica; Alaska
• Online Access: https://dx.doi.org/10.5061/dryad.pzgmsbcj3; https://datadryad.org/stash/dataset/doi:10.5061/dryad.pzgmsbcj3

It has been previously suggested that snow algal species within the genus Sanguina (S. nivaloides and S. aurantia) show no population structure despite being found globally (S. nivaloides) or throughout the Northern Hemisphere (S. aurantia). However, systematic biogeographic research into global distributions is lacking due to few genetic and no genomic resources for these snow algae. Here, using all publicly available and previously unpublished Sanguina sequences of the Internal Transcribed Spacer 2 region, we investigate if this purported lack of population structure within Sanguina species is supported by additional evidence. Using a minimum entropy decomposition (MED) approach to examine fine-scale genetic population structure, we find that these snow algae populations are largely distinct regionally and have some interesting biogeographic structuring. This is in opposition to the currently accepted idea that Sanguina species lack any observable population structure across their vast ranges and ... : We analyzed all available and verified ITS2 sequences at the time of analysis from Sanguina species from GenBank, SRA, and supplemental information from associated publications. We chose to analyze the ITS2 region as opposed to 18S or other gene targets because ITS2 has the most available data and ITS regions have great potential for species level population analysis for algae (An et al. 1999). We gathered the following Sanger sequences: 56 sequences from Segawa et al. (2018) collected from Alaska (USA), Svalbard (Norway), and Antarctica; 48 sequences from Procházková et al. (2019) from Austria, Italy, Slovakia, Switzerland, Norway, Colorado (USA), Argentina, and Antarctica; 29 sequences (Brown, unpublished using the primers ITS1-ITS4) from Lyman Basin, Washington (USA; 48º10’21” N, 120º53’50” W, 1880 m asl) and Niwot Ridge, Colorado (USA; 40º02’56” N, 105º34’51” W, 3514 m asl). Further, we gathered locus-targeted Illumina MiSeq sequence data: 1,600 sequences (Brown et al. 2016) from Washington (USA) and ...