Data from: Responses of neurogenesis and neuroplasticity related genes to elevated CO2 levels in the brain of three teleost species ...

• Main Authors: Lai, Floriana, Fagernes, Cathrine E., Bernier, Nicholas J., Miller, Gabrielle M., Munday, Philip L., Jutfelt, Fredrik, Nilsson, Göran E.
• Format: Dataset
• Language: English
• Published: Dryad 2017
• Subjects: elevated CO2; cinnamon anemonefish; spiny damselfish; Neurogenesis; Amphiprion melanopus; Acanthochromis polyacanthus; Gasterosteus aculeatus; three spined stickleback; Rasmussen; Ocean acidification
• Online Access: https://dx.doi.org/10.5061/dryad.pn397; https://datadryad.org/stash/dataset/doi:10.5061/dryad.pn397

The continuous increase of anthropogenic CO2 in the atmosphere resulting in ocean acidification has been reported to affect brain function in some fishes. During adulthood, cell proliferation is fundamental for fish brain growth and for it to adapt in response to external stimuli, such as environmental changes. Here we report the first expression study of genes regulating neurogenesis and neuroplasticity in brains of three-spined stickleback (Gasterosteus aculeatus), cinnamon anemonefish (Amphiprion melanopus) and spiny damselfish (Acanthochromis polyacanthus) exposed to elevated CO2. The mRNA expression levels of the neurogenic differentiation factor (NeuroD) and doublecortin (DCX) were upregulated in three-spined stickleback exposed to high-CO2 compared with controls, while no changes were detected in the other species. The mRNA expression levels of the proliferating cell nuclear antigen (PCNA) and the brain-derived neurotrophic factor (BDNF) remained unaffected in the high-CO2 exposed groups compared to ... : Lai et al., 2017_DataSets_Biology letterCp values and priming efficiencies were calculated using the second derivative maximum method (Roche Lightcycler 480; Rasmussen, 2001) and the LinRegPCR software (Ruijter et al., 2009), respectively. Subsequently, relative mRNA expression levels were calculated using the following formula: Expression of target gene = (Ega^Cpga/Etar^Cptar) Where ga is the geometric average of the two reference genes; tar is the target gene of interest, E is priming efficiency and Cp is the crossing point. Since duplicate cDNA syntheses were performed, and each of these were analysed in duplicates in the qPCR analyses, four data points were present for each original sample for each primer pair used, and their means were used in the mRNA expression calculations. ...