Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ...
Secondary metabolites produced by primary producers have a wide range of functions as well as indirect effects outside the scope of their direct target. Research suggests that protease inhibitors produced by cyanobacteria influence grazing by herbivores and may also protect against parasites of cyan...
Main Author: | |
---|---|
Format: | Dataset |
Language: | English |
Published: |
Dryad
2023
|
Subjects: | |
Online Access: | https://dx.doi.org/10.5061/dryad.nzs7h44xq https://datadryad.org/stash/dataset/doi:10.5061/dryad.nzs7h44xq |
id |
ftdatacite:10.5061/dryad.nzs7h44xq |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.5061/dryad.nzs7h44xq 2024-06-09T07:44:54+00:00 Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ... Sanchez, Kristel 2023 https://dx.doi.org/10.5061/dryad.nzs7h44xq https://datadryad.org/stash/dataset/doi:10.5061/dryad.nzs7h44xq en eng Dryad Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 FOS Biological sciences ascospore cyanopeptolins Cyanobacteria Pathogens Dataset dataset 2023 ftdatacite https://doi.org/10.5061/dryad.nzs7h44xq 2024-05-13T11:16:38Z Secondary metabolites produced by primary producers have a wide range of functions as well as indirect effects outside the scope of their direct target. Research suggests that protease inhibitors produced by cyanobacteria influence grazing by herbivores and may also protect against parasites of cyanobacteria. In this study we asked whether those same protease inhibitors produced by cyanobacteria also can influence interactions of herbivores with their parasites. We used the Daphnia-Metschnikowia zooplankton host-fungal parasite system to address this question because it is well-documented that cyanobacteria protease inhibitors suppress trypsin and chymotrypsin in the gut of Daphnia, and because it is known that Metschnikowia infects via the gut. We tested the hypothesis that Daphnia gut proteases are necessary for Metschnikowia spores to be released from their asci. We then also tested whether diets that decrease trypsin and chymotrypsin activity in the guts of Daphnia lead to lower levels of infection. Our ... : Host-parasite system Our study used 11 clones of Daphnia magna, which is a common species in ponds and lakes, especially in Europe. These clones come from different populations and have been used in studies focusing on the impact of cyanobacterial diets on Daphnia gut digestive enzymes (e.g., Schwarzenberger et al., 2021; see supplementary material and Table S1 for additional information about these clones). We used the common fungal parasite Metschnikowia bicuspidata (“Standard” isolate, originally isolated from Baker Lake in Barry County, Michigan). Daphnia become infected after consuming transmission spores they encounter in the water column when feeding. Infection takes place when the needle-shaped spore crosses the gut barrier and is not fought off by a host hemocyte response (Metschnikoff, 1884; Stewart Merrill and Cáceres, 2018). Once infection has taken hold, the fungus replicates within the hemolymph of the host (Stewart Merrill and Cáceres, 2018). The parasite reduces the fecundity and lifespan of ... Dataset Baker Lake DataCite Metadata Store (German National Library of Science and Technology) The Needle ENVELOPE(-64.047,-64.047,63.267,63.267) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
FOS Biological sciences ascospore cyanopeptolins Cyanobacteria Pathogens |
spellingShingle |
FOS Biological sciences ascospore cyanopeptolins Cyanobacteria Pathogens Sanchez, Kristel Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ... |
topic_facet |
FOS Biological sciences ascospore cyanopeptolins Cyanobacteria Pathogens |
description |
Secondary metabolites produced by primary producers have a wide range of functions as well as indirect effects outside the scope of their direct target. Research suggests that protease inhibitors produced by cyanobacteria influence grazing by herbivores and may also protect against parasites of cyanobacteria. In this study we asked whether those same protease inhibitors produced by cyanobacteria also can influence interactions of herbivores with their parasites. We used the Daphnia-Metschnikowia zooplankton host-fungal parasite system to address this question because it is well-documented that cyanobacteria protease inhibitors suppress trypsin and chymotrypsin in the gut of Daphnia, and because it is known that Metschnikowia infects via the gut. We tested the hypothesis that Daphnia gut proteases are necessary for Metschnikowia spores to be released from their asci. We then also tested whether diets that decrease trypsin and chymotrypsin activity in the guts of Daphnia lead to lower levels of infection. Our ... : Host-parasite system Our study used 11 clones of Daphnia magna, which is a common species in ponds and lakes, especially in Europe. These clones come from different populations and have been used in studies focusing on the impact of cyanobacterial diets on Daphnia gut digestive enzymes (e.g., Schwarzenberger et al., 2021; see supplementary material and Table S1 for additional information about these clones). We used the common fungal parasite Metschnikowia bicuspidata (“Standard” isolate, originally isolated from Baker Lake in Barry County, Michigan). Daphnia become infected after consuming transmission spores they encounter in the water column when feeding. Infection takes place when the needle-shaped spore crosses the gut barrier and is not fought off by a host hemocyte response (Metschnikoff, 1884; Stewart Merrill and Cáceres, 2018). Once infection has taken hold, the fungus replicates within the hemolymph of the host (Stewart Merrill and Cáceres, 2018). The parasite reduces the fecundity and lifespan of ... |
format |
Dataset |
author |
Sanchez, Kristel |
author_facet |
Sanchez, Kristel |
author_sort |
Sanchez, Kristel |
title |
Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ... |
title_short |
Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ... |
title_full |
Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ... |
title_fullStr |
Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ... |
title_full_unstemmed |
Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system ... |
title_sort |
inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a daphnia host-parasite system ... |
publisher |
Dryad |
publishDate |
2023 |
url |
https://dx.doi.org/10.5061/dryad.nzs7h44xq https://datadryad.org/stash/dataset/doi:10.5061/dryad.nzs7h44xq |
long_lat |
ENVELOPE(-64.047,-64.047,63.267,63.267) |
geographic |
The Needle |
geographic_facet |
The Needle |
genre |
Baker Lake |
genre_facet |
Baker Lake |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.nzs7h44xq |
_version_ |
1801373748414119936 |