Sequence alignments for ITS, rpl16, and trnL-trnF ...

• Main Author: Hedenäs, Lars
• Format: Dataset
• Language: English
• Published: Dryad 2021
• Subjects: FOS Biological sciences; Greenland; Jan Mayen; Norway; Svalbard; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.5061/dryad.nvx0k6dtg; https://datadryad.org/stash/dataset/doi:10.5061/dryad.nvx0k6dtg

The two data files consist of sequence alignments in FASTA format. The file 'Distichium_alignment_ITS.txt' is the alignment used for the analysis resulting in the network in Fig. 1A in the paper and the file 'Distichium_alignment_3 markers.txt' is the alignment used for the analysis resulting in the network in Fig. 1B. The GenBank numbers corresponding with the sequence numbers can be found in Appendix 1 in the paper. ... : The core portion of this study includes 86 samples of D. capillaceum (ITS, rpl16, trnL-trnF). Sixty-nine come from Sweden, 16 from mainland Norway, and one from Svalbard. The samples cover its phenotypic variation in Scandinavia. To explore Distichium relationships in a wider context I downloaded Internal Transcribed Spacers 1 and 2 (ITS) sequences from GenBank for eight additional D. capillaceum specimens, from mainland Norway (3 samples), Jan Mayen (1), Svalbard (1), Greenland (2), and Antarctica (1), and two sequences of D. hagenii Ryan ex H. Philib. The beginnings of the downloaded ITS sequences were less complete than the newly generated ones. Two specimens of D. inclinatum were used as outgroup based on its position as sister to the other two species of the genus. Molecular methods Total DNA was extracted using the Mag-Bind® Plant DNA Plus 96 Kit (Omega Bio-tek) with the KingFisher Flex and Duo magnetic particle processors. Double stranded DNA templates were prepared by polymerase chain reaction (PCR). ...