Data from: Larval development in the Pacific oyster and the impacts of ocean acidification: differential genetic effects in wild and domesticated stocks ...

• Main Authors: Durland, Evan, De Wit, Pierre, Meyer, Eli, Langdon, Chris
• Format: Dataset
• Language: English
• Published: Dryad 2021
• Subjects: PoolSeq; FOS Biological sciences; Crassostrea gigas; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.5061/dryad.mgqnk98vw; https://datadryad.org/stash/dataset/doi:10.5061/dryad.mgqnk98vw

The adaptive capacity of marine calcifiers to ocean acidification (OA) is a topic of great interest to evolutionary biologists and ecologists. Previous studies have provided evidence to suggest that larval resilience to high pCO­2 seawater for these species is a trait with a genetic basis and variability in natural populations. To date, however, it remains unclear how the selective effects of OA occur within the context of complex genetic interactions underpinning larval development in many of the most vulnerable taxa. Here we evaluated phenotypic and genetic changes during larval development of Pacific oysters (Crassostrea gigas) reared in ambient (~ 400 µatm) and high (~ 1600 µatm) pCO2 conditions, both in domesticated and naturalized ‘wild’ oysters from the Pacific Northwest, USA. Using pooled DNA samples, we determined changes in allele frequencies across larval development, from early “D-stage” larvae to metamorphosed juveniles (spat), in both groups and environments. Domesticated larvae had ~ 26% fewer ... : Pooled samples of oyster larvae (n=200-3000) were collected at 2 and 22 days post fertilization. DNA was extracted in bulk, converted to 2bRAD libraries (BCGI enzyme) and sequenced on an Illumina Hiseq (SE 50bp reads). Samples represent two oyster populations (MBP or Wild) from the Pacific Northwest USA. Larvae from each population was reared in ambient (~400 pCO2) and acidified (~1600 pCO2) conditions from fertilization to settlement (22 days post fertilization). ...