Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus ...

• Main Authors: Pascoal, Sonia, Carvalho, Gary, Vasieva, Olga, Hughes, Roger, Cossins, Andrew, Fang, Yongxiang, Ashelford, Kevin, Olohan, Lisa, Barroso, Carlos, Mendo, Sonia, Creer, Simon
• Format: Dataset
• Language: English
• Published: Dryad 2013
• Subjects: Genomics/Proteomics; Nucella lapillus; Gene Structure and Function; Dogwhelk
• Online Access: https://dx.doi.org/10.5061/dryad.m1063; https://datadryad.org/stash/dataset/doi:10.5061/dryad.m1063

Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption (ED) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapillus (imposex), caused by organotins, provides one of the most clearcut ecological examples of anthropogenically induced ED in aquatic ecosystems. To identify the underpinning mechanisms of imposex for this ‘nonmodel’ species, we combined Roche 454 pyrosequencing with custom oligoarray fabrication inexpensively to both generate gene models and identify those responding to chronic tributyltin (TBT) treatment. The results supported the involvement of steroid, neuroendocrine peptide hormone dysfunction and retinoid mechanisms, but suggested additionally the involvement of putative peroxisome proliferator–activated receptor (PPAR) pathways. Application of rosiglitazone, a well-known vertebrate PPARγ ligand, to ... : Nucella_CoAssemblyCo-assembly data: list of contigs and singletonsSRA021021Control and TBT-exposed libraries: sequencing results ...