Data from: Metabarcoding for the parallel identification of several hundred predators and their preys: application to bat species diet analysis ...

• Main Authors: Galan, Maxime, Pons, Jean-Baptiste, Tournayre, Orianne, Pierre, Eric, Leuchtmann, Maxime, Pontier, Dominique, Charbonnel, Nathalie
• Format: Dataset
• Language: English
• Published: Dryad 2017
• Subjects: Myotis emarginatus; high-throughput sequencing HTS; Rhinolophus ferrumequinum; Myotis bechsteinii; Plecotus auritus; False positives; Barbastella barbastellus; Myotis myotis; Miniopterus schreibersii; Myotis alcathoe; Rhinolophus euryale; Pipistrellus kuhlii; Myotis daubentonii; Myotis mystacinus; Eptesicus serotinus; Pipistrellus pipistrellus; Chiroptera; Myotis nattereri; Rhinolophus hipposideros
• Online Access: https://dx.doi.org/10.5061/dryad.kv02g; https://datadryad.org/stash/dataset/doi:10.5061/dryad.kv02g

Assessing diet variability is of main importance to better understand the biology of bats and design conservation strategies. Although the advent of metabarcoding has facilitated such analyses, this approach does not come without challenges. Biases may occur throughout the whole experiment, from fieldwork to biostatistics, resulting in the detection of false negatives, false positives or low taxonomic resolution. We detail a rigorous metabarcoding approach based on a short COI minibarcode and two-step PCR protocol enabling the ‘all at once’ taxonomic identification of bats and their arthropod preys for several hundreds of samples. Our study includes faecal pellets collected in France from 357 bats representing 16 species, as well as insect mock communities that mimic bat meals of known composition, negative and positive controls. All samples were analysed using three replicates. We compare the efficiency of DNA extraction methods and we evaluate the effectiveness of our protocol using identification success, ... : MiSeq raw sequences of the COI minibarcode from the faecal pellets of 357 bats from Western France (part 1 to 2)This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each faecal pellet in triplicate using the MiSeq platform. The 1162 multiplexed PCR products were indexed using both forward and reverse indices. The list of the 357 multiplexed samples and the 24 positive and 58 negative controls are provided in the following XLSX file titled: Information concerning the samples multiplexed in the MiSeq Run.MiSeq_Reads_COI_Bat_ faecal_samples_part1.zipMiSeq raw sequences of the COI minibarcode from the faecal pellets of 357 bats from Western France (part 2 to 2)This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each faecal pellet in triplicate using the MiSeq platform. The 1162 multiplexed PCR products were indexed using both forward and reverse indices. The list of the 357 multiplexed samples and the 24 positive ...