Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas ...
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly und...
| Main Authors: | , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Dryad
2018
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.kn45n49 https://datadryad.org/stash/dataset/doi:10.5061/dryad.kn45n49 |
| Summary: | Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a Km of 6.2 mM and a Vmax of 3.3 nM min−1. The transcellular transfer of Ca2+ accounts for approximately 60% of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the ... : Electronic data for kinetics studyThe electronic raw data for kinetics experimentElectronic data caloxinElectronic data ORMElectronic data orm.xlsxElectronic data ouabainElectronic data vanadateElectronic data verapamilDPM kinetics studyDPM kinetics.xlsxDPM caloxinDPM ORMDPM ouabainDPM vanadateDPM verapamil ... |
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